Splenocyte, BMMC, eosinophil을 통해 본 丹蔘이 천식 관련 염증세포에 미치는 영향
Effects of Salviae miltiorrhizae on Inflammatory Cells Associated with Asthma via Splenocyte, BMMC, Eosinophil
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Objectives : Asthma is a chronic inflammatory disorder of the airways by many cells such as mast cells, Th2 lymphocyte and eosinophils. The present study was aimed to evaluate the effects of Salviae miltiorrhizae(SM) on T cell cytokine production, mast cells, eosinophils. Methods : We screened 13 herbs to find compounds with potential to control Th cytokine production, using concanavalin A (con A)-activated splenocyte cultures. Con A-activated IFN-γ and IL-4 levels in supernatants of splenocyte cultures. Bone marrow derived mast cell (BMMC)s were incubated with SM and then the expressions of membrane proteins of BMMC were analyzed by fluorescence activated cell sorter (FACS). BALB/c mice sensitized to ovalbumin (OVA) were challenged with aerosolized OVA for 6weeks. During the last weeks some mice were treated with SM. Then eosinophils in bronchoalveolar lavage fluid (BALF) were counted and pathologic changes of lung tissue were observed with hematoxylin-eosin stain. Results : SM increased IFN-γ level on splenocyte culture significantly. But SM had no significant effects on expressions of ICAM-1, CD62L, integrin α4, c-kit, IL-3 receptor, CD11a, IgE receptor of BMMC. And SM treatment significantly inhibited eosinophil infiltrates in BALF and peribronchial lung inflammation. Conculusions : The present data suggested that SM may have an effect on Th cytokine secretion and eosinophils associated with asthma responses. Therefore SM might be of therapeutic value in treating asthma.
Abstract
Objectives : Asthma is a chronic inflammatory disorder of the airways by many cells such as mast cells, Th2 lymphocyte and eosinophils. The present study was aimed to evaluate the effects of Salviae miltiorrhizae(SM) on T cell cytokine production, mast cells, eosinophils. Methods : We screened 13 herbs to find compounds with potential to control Th cytokine production, using concanavalin A (con A)-activated splenocyte cultures. Con A-activated IFN-γ and IL-4 levels in supernatants of splenocyte cultures. Bone marrow derived mast cell (BMMC)s were incubated with SM and then the expressions of membrane proteins of BMMC were analyzed by fluorescence activated cell sorter (FACS). BALB/c mice sensitized to ovalbumin (OVA) were challenged with aerosolized OVA for 6weeks. During the last weeks some mice were treated with SM. Then eosinophils in bronchoalveolar lavage fluid (BALF) were counted and pathologic changes of lung tissue were observed with hematoxylin-eosin stain. Results : SM increased IFN-γ level on splenocyte culture significantly. But SM had no significant effects on expressions of ICAM-1, CD62L, integrin α4, c-kit, IL-3 receptor, CD11a, IgE receptor of BMMC. And SM treatment significantly inhibited eosinophil infiltrates in BALF and peribronchial lung inflammation. Conculusions : The present data suggested that SM may have an effect on Th cytokine secretion and eosinophils associated with asthma responses. Therefore SM might be of therapeutic value in treating asthma.
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