三子化痰煎이 천식모델 생쥐의 면역세포에 미치는 영향
Effects of Samjawhadam-jeon on Immune Cells in Ovalbumin-induced Asthmatic Mice
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Objective : The purpose of this study was to evaluate the effect of Samjawhadam-jeon (SJHDJ; 三子化痰煎) on immune cells in OVA-induced asthmatic mice. Material and Methods : C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four times a week) for asthma induction. Two experimental groups were treated with different concentrations of SJHDJ group (400 ㎎/㎏) extracts and cyclosporin A (10 ㎎/㎏) for the latter 8 weeks. At the end of the experiment, the mouse lung, peripheral lymph node (PLN) and spleen were removed and immune cells were analyzed by flow cytometer. Results : Lung weight, total cells in lung, PLN, and spleen of the SJHDJ group decreased significantly compared with that of the control group. Number of CD3e+/CD69+, CD3e+/DX5+ cells in lung, PLN and spleen, number of CD3+ cells in PLN and spleen, number of CD3e-/CCR3+ cells in lung and PLN, and number of B220+/IgE+ cells in PLN of the SJHDJ group decreased compared with that of the control group. Number of CD4+/CD25+ cells in PLN and spleen of the SJHDJ group increased compared with that of the control group. Conclusion : These results demonstrate that SJHDJ will be a desirable alternative therapy for allergic asthma by inhibiting the expression of immune cells.
Abstract
Objective : The purpose of this study was to evaluate the effect of Samjawhadam-jeon (SJHDJ; 三子化痰煎) on immune cells in OVA-induced asthmatic mice. Material and Methods : C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four times a week) for asthma induction. Two experimental groups were treated with different concentrations of SJHDJ group (400 ㎎/㎏) extracts and cyclosporin A (10 ㎎/㎏) for the latter 8 weeks. At the end of the experiment, the mouse lung, peripheral lymph node (PLN) and spleen were removed and immune cells were analyzed by flow cytometer. Results : Lung weight, total cells in lung, PLN, and spleen of the SJHDJ group decreased significantly compared with that of the control group. Number of CD3e+/CD69+, CD3e+/DX5+ cells in lung, PLN and spleen, number of CD3+ cells in PLN and spleen, number of CD3e-/CCR3+ cells in lung and PLN, and number of B220+/IgE+ cells in PLN of the SJHDJ group decreased compared with that of the control group. Number of CD4+/CD25+ cells in PLN and spleen of the SJHDJ group increased compared with that of the control group. Conclusion : These results demonstrate that SJHDJ will be a desirable alternative therapy for allergic asthma by inhibiting the expression of immune cells.
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