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천식모델생쥐에서 宣肺湯의 면역조절 효과

Effects of Seonpye-tang on OVA-Induced Asthmatic Mice

박양춘|이주관
한의병리학회 (The Society of Pathology in Korean Medicine)|2009.04|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제23권 제2호|pp. 457-463

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키워드

cytokineantioxidative effectasthmaimmune cellSeonpye-tang
주제분류한의학

초록

This study aimed to evaluate the anti-asthmatic effects of Seonpye-tang (SPT) using OVA-induced asthmatic mice model. Scavenging activity of SPT on DPPH free radical and SOD-like activity of SPT were measured at final concentration 62.5, 125, 250, 500 (㎍/㎖). RBL-2H3 cells were treated with DNP IgE for 24hr, and treated with SPT (1, 10, 100 ㎍/㎖) for 1hr, followed by treatment with DNP-HSA for 1hr at 37℃. The level of IL-4 and TNF-α were measured by ELISA. Asthmatic mice model was conducted by repeated challenge of OVA using C57BL/6 mice. Each group was treated with distilled water, SPT (400 ㎎/㎏ and 200 ㎎/㎏) extract or cyclosporin A (10 ㎎/㎏) for the later 8 weeks. Immune cells subpopulation, eotaxin, IL-5 and TNF-α in BALF were analyzed. SPT dose-dependently increased Scavenging activity on DPPH free radical and SOD-like activity. SPT significantly ameliorated the increase of total cells number and eosinophil including of immune cell subpopulation of CD3+/CD69+, CCR3+, B220+/CD22+, B220+/CD45+ and B220+/IgE+ cells in BALF comparing to control group. Eotaxin and IL-5 level in BALF were significantly decreased by SPT. These results strongly suggest that SPT would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanisms using variant disease model in the future.

Abstract

This study aimed to evaluate the anti-asthmatic effects of Seonpye-tang (SPT) using OVA-induced asthmatic mice model. Scavenging activity of SPT on DPPH free radical and SOD-like activity of SPT were measured at final concentration 62.5, 125, 250, 500 (㎍/㎖). RBL-2H3 cells were treated with DNP IgE for 24hr, and treated with SPT (1, 10, 100 ㎍/㎖) for 1hr, followed by treatment with DNP-HSA for 1hr at 37℃. The level of IL-4 and TNF-α were measured by ELISA. Asthmatic mice model was conducted by repeated challenge of OVA using C57BL/6 mice. Each group was treated with distilled water, SPT (400 ㎎/㎏ and 200 ㎎/㎏) extract or cyclosporin A (10 ㎎/㎏) for the later 8 weeks. Immune cells subpopulation, eotaxin, IL-5 and TNF-α in BALF were analyzed. SPT dose-dependently increased Scavenging activity on DPPH free radical and SOD-like activity. SPT significantly ameliorated the increase of total cells number and eosinophil including of immune cell subpopulation of CD3+/CD69+, CCR3+, B220+/CD22+, B220+/CD45+ and B220+/IgE+ cells in BALF comparing to control group. Eotaxin and IL-5 level in BALF were significantly decreased by SPT. These results strongly suggest that SPT would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanisms using variant disease model in the future.

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