본문 바로가기
KERD
논문KCI 등재

상황버섯 가수분해물의 피부 항노화 효능에 대한 연구

Studies on Skin Anti-aging Efficacy of Hydrolysate from Phellinus igniarius

2

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류한의학

초록

Objective : The object of this study was to assess the efficacy of hydrolysate from Phellinus igniarius(HPI) on anti-aging activities in vitro measurement and mini clinical study performed on 5 subjects. Methods : To evaluate skin anti-aging efficacy of HPI, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, type I collagen synthesis, inhibition of nitric oxide(NO) production, inhibiton of tyrosinase, hyaluronan synthase(HAS)2, 3 mRNA expression were measured in vitro. Also, mini clinical study of skin hydration was performed on 5 subjects using HPI in distilled water(DW) and 1,3-butylene glycol diluted solution(30% in DW). Results : 1. DPPH radical scavenging activity of HPI was increased in a dose-dependant. 2. TypeⅠ collagen synthesis was increased in 50, 100 and 500㎍/㎖ of HPI. 3. NO production was not inhibited in all concentrations of HPI. 4. Tyrosinase was inhibited in 500, 1000, 2500 and 5000㎍/㎖ of HPI. 5. HAS2 mRNA expression was increased in 50, 100, 150 and 200㎍/㎖ of HPI, HAS3 mRNA expression was increased in 100, 150, and 200㎍/㎖ of HPI. 6. In the mini clinical study of 5 subjects, there was a difference in skin hydration over time for each solutions, but it was not statistically siginificant. Conclusions : HPI increased DPPH radical scavenging activity, type I collagen synthesis, and HAS2, HAS3 mRNA expression. HPI also suppressed tyrosinase. The findings of this study suggest that HPI can be used as an skin anti-aging material.

Abstract

Objective : The object of this study was to assess the efficacy of hydrolysate from Phellinus igniarius(HPI) on anti-aging activities in vitro measurement and mini clinical study performed on 5 subjects. Methods : To evaluate skin anti-aging efficacy of HPI, 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity, type I collagen synthesis, inhibition of nitric oxide(NO) production, inhibiton of tyrosinase, hyaluronan synthase(HAS)2, 3 mRNA expression were measured in vitro. Also, mini clinical study of skin hydration was performed on 5 subjects using HPI in distilled water(DW) and 1,3-butylene glycol diluted solution(30% in DW). Results : 1. DPPH radical scavenging activity of HPI was increased in a dose-dependant. 2. TypeⅠ collagen synthesis was increased in 50, 100 and 500㎍/㎖ of HPI. 3. NO production was not inhibited in all concentrations of HPI. 4. Tyrosinase was inhibited in 500, 1000, 2500 and 5000㎍/㎖ of HPI. 5. HAS2 mRNA expression was increased in 50, 100, 150 and 200㎍/㎖ of HPI, HAS3 mRNA expression was increased in 100, 150, and 200㎍/㎖ of HPI. 6. In the mini clinical study of 5 subjects, there was a difference in skin hydration over time for each solutions, but it was not statistically siginificant. Conclusions : HPI increased DPPH radical scavenging activity, type I collagen synthesis, and HAS2, HAS3 mRNA expression. HPI also suppressed tyrosinase. The findings of this study suggest that HPI can be used as an skin anti-aging material.

참고문헌 (60)

  1. 김인덕, "한방원료의 초임계 추출을 이용한 항노화 및 주름개선 효과", KSBB Journal, 23(6), pp.529~534 (2008), 한국생물공학회
  2. Nam HJ, "Aging and skin aging", J Korean Med Ophthalmol Otolaryngol Dermatol, 17(1), pp.16~33 (2004)
  3. 송환, "영지버섯에서 추출한 포자오일의 항노화 및 보습 효능", 융합정보논문지, 10(5), pp.232~238 (2020), 중소기업융합학회[DOI]
  4. Kim SH, "Effect of the Cosmetics Products Containing Morinda citrifolia on the 30-50 Middle-aged Women's Skin", The Korean Beauty Management Society, 4(1), pp.1~7 (2016)
  5. 박혜수, "아토피성 피부로 건조함을 가진 대상자에 대한 환삼덩굴추출물 함유 기능성 화장품의 유효성 및 안전성을 평가하기 위한 임상적 연구", 한방안이비인후피부과학회지, 32(2), pp.24~58 (2019), 대한한방안이비인후피부과학회[DOI]
  6. 소순영, "비자 및 초두구 추출물의 코스메슈티칼 특성 및 이를 포함한 마스크팩 제형특성", 한국산학기술학회논문지, 21(8), pp.36~43 (2020), 한국산학기술학회[DOI]
  7. 김계엽, "표고버섯 추출물의 항혈소판 응집 및 항혈전 효과", 동의생리병리학회지, 27(2), pp.239~245 (2013), 한의병리학회
  8. 서명성, "표고버섯 추출물의 화장품약리활성 검증과 천연화장품 소재로써의 활용에 관한 연구", 한국응용과학기술학회지, 35(4), pp.1003~1012 (2018), 한국응용과학기술학회[DOI]
  9. 김아름, "상황버섯 추출물의 항산화 및 성분분석", 대한화장품학회지, 37(4), pp.309~318 (2011), 사단법인 대한화장품학회
  10. Lee SJ, "Dohae Bonchogangmok", pp.500~505 (1975), Komoonsa
  11. Pyo MY, "Effects of phellinus linteus extracts on the humoral immune response in normal and cyclophosphamide-treated mice", The Journal of Applied Pharmacology, 9(3), pp.194~200 (2001)
  12. Rhee YK, "In vitro and in vivo Antitumor Activity of the Fruit Body of Phellinus linteus", Korean J. Food Sci. Technol, 32(2), pp.477~480 (2000)
  13. 이연희, "발아현미상황버섯이 아토피피부염에 미치는 영향", 한국미용학회지, 13(2), pp.514~519 (2007), 한국미용학회
  14. Song CH, "Immuno-stimulating activity of phellinus linteus", The Korean Journal of Mycology, 26(1), pp.86~90 (1998)
  15. 서진희, "상황버섯의 복용이 점증적 최대운동부하시 유산소성 운동능력과 혈중 젖산 농도에 미치는 영향", 한국체육학회지, 47(2), pp.499~505 (2008), 한국체육학회
  16. Jung ME, "Biochemical and Histological Effects of Phellinus linteus Methanol Extract on Liver Lipid Metabolism of Rats Fed CCl4and High Fat", J. Korean Soc. Food Sci. Nutr, 30(2), pp.331~337 (2001)
  17. 정용준, "상황버섯과 발아현미상황버섯 열수추출물의 Murin Macrophage RAW 264.7 세포에서 항염증 반응 비교", 한국약용작물학회지, 17(2), pp.97~101 (2009), 한국약용작물학회
  18. Ji JH, "Antimutagenic and Cytotoxicity Effects of Phellinus linteus Extracts", J. Korean Soc. Food Sci. Nutr, 29(2), pp.322~328 (2000)
  19. 윤우식, "상황버섯 추출물이 항염증 및 항알레르기, 항산화, 주름제거에 미치는 실험적 연구", 한방안이비인후피부과학회지, 23(1), pp.75~93 (2010), 대한한방안이비인후피부과학회
  20. 곽병문, "Component analysis of four-part extracts from Chamaecyparis obtusa Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells", Journal of exercise rehabilitation, 15(5), pp.723~730 (2019), 한국운동재활학회[DOI]
  21. Han LL, "Studies on skin moisturizing and anti-inflammatory efficacy of peptide and amino acid hydrolysate from ginseng seed", master, pp.2017
  22. KDA Textbook Editing Board, "Dermatology", pp.2~51 (2014), Medbook
  23. 김주은, "금전초 추출물의 케라티노사이트 내 collagen 합성능 및 MMPs 억제효과", 한국응용과학기술학회지, 37(4), pp.820~829 (2020), 한국응용과학기술학회
  24. 김태준, "內托千金散이 RAW 264.7 대식세포주에서 항염증 활성에 미치는 영향", 한방안이비인후피부과학회지, 31(1), pp.12~21 (2018), 대한한방안이비인후피부과학회[DOI]
  25. Chen CY, "Tyrosinase inhibition free radical scavenging antimicroorganism and anticancer proliferation activities of sapindus mukorossi extracts", Journal of the Taiwan Institute of Chemical Engineers, 41(2), pp.129~135 (2010)
  26. Triggs-Raine B, "Biology of hyaluronan : Insights from genetic disorders of hyaluronan metabolism", World J Biol Chem, 6(3), pp.110~120 (2015)
  27. 김현숙, "인삼열매 에탄올 추출물의 화장품 소재로서 항산화, 항염 효과", 아시안뷰티화장품학술지, 18(3), pp.389~397 (2020), 한국피부과학연구원[DOI]
  28. 윤지혜, "기린초(Sedum kamtschaticum Fisch.) 추출 분획물의 피부 미백효과 연구", 한국응용과학기술학회지, 37(5), pp.1239~1247 (2020), 한국응용과학기술학회
  29. 심중현, "자외선 조사에 의해 노화된 인간각질형성세포에서 sulfuretin의 항노화 효능", 아시안뷰티화장품학술지, 18(3), pp.265~272 (2020), 한국피부과학연구원[DOI]
  30. 손은화, "상황버섯의 기술개발 동향과 시장분석", KSBB Journal, 23(2), pp.109~117 (2008), 한국생물공학회
  31. 강미영, "시장성이 없는 품질의 갈변 표고버섯(Lentinus edodes) 추출물의 항산화 활성", 한국식품과학회지, 36(4), pp.648~654 (2004), 한국식품과학회
  32. 김민선, "신령버섯 생물전환 추출물이 인간 섬유아세포의 콜라겐 합성 및 Matrix Metalloproteinase-1 생성에 미치는 영향", KSBB Journal, 34(1), pp.49~53 (2019), 한국생물공학회[DOI]
  33. 김정은, "신령버섯에서 분리된 Glucosylceramide 및 Sterol의 피부 세포 개선 효과", 대한화장품학회지, 46(2), pp.105~117 (2020), 사단법인 대한화장품학회[DOI]
  34. 장혜림, "추출용매에 따른 이슬송이버섯(Lentinula edodes GNA01) 추출물의 항산화 활성", 한국식품영양학회지, 30(1), pp.51~58 (2017), 한국식품영양학회[DOI]
  35. 홍원규, "소아 아토피 피부염에서 발아현미 상황버섯의 임상적 치료효과", 대한본초학회지, 23(1), pp.103~108 (2008), 대한본초학회
  36. 박석규, "상황버섯의 화학성분 및 생리활성 효과", 한국식품영양과학회지, 35(6), pp.524~529 (2006), 한국식품영양과학회
  37. Choi CH, "Saengtaeboncho", pp.366~380 (2018), Fishwood
  38. Dong-A Editing Board, "Dong-A Chinese character Korean Dictionary", pp.1443~1444 (1989), Dong-A Publishing
  39. Choo J, "Bongyeongsojeung", pp.657~658 (2001), Medibook
  40. Ko BS, "Sinpyeonjungyagdaesajeon", pp.1479~1480 (1982), Sinmunpung Publisher
  41. Heo J, "Donguibogam", pp.2139~2140 (2006), Donguibogam Publisher
  42. Ko WS, "Text of Traditional Korean Dermatology & Surgery", pp.27~142 (2007), Seonu
  43. Kwak BM, "Studies on the anti-inflammatory effect of the supercritical fluid extraction from Chamaecyparis obtusa Endl", doctor, pp.2019
  44. Rachmawati D, "Utilization of papain as feed additive in the fish feed on activity of digestive enzymes, contents of nutrient and minerals of Sangkuriang catfish", AACL Bioflux, 13(5), pp.2738~2744 (2020)
  45. Choi C, "Purificaton and characteristics of bromelain from korean pineapple", J. Korean Agric. Chem. Soc, 35(1), pp.23~29 (1992)
  46. 김소정, "LPS로 유도된 RAW 264.7 세포에서 오성탕(五聖湯) 추출물의 항염증 및 항산화 연구", 한방안이비인후피부과학회지, 33(1), pp.1~11 (2020), 대한한방안이비인후피부과학회[DOI]
  47. 최관호, "顚倒散의 항산화 효과에 관한 실험적 연구", Journal of Pharmacopuncture, 11(1), pp.143~147 (2008), 대한약침학회
  48. Yang B, "Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts", Int J Mol Med, 23(4), pp.555~560 (2009)
  49. Tsuji-Naito K, "α- Lipoic acid induces collagen biosynthesis involving prolyl hydroxylase expression via activation of TGF-β-Smad signaling in human dermal fibroblasts", Connect Tissue Res, 51(5), pp.378~387 (2010)
  50. Huang C, "Blocking activator protein-1 activity, but not activating retinoic acid response element, is required for the antitumor promotion effect of retinoic acid", Proc Natl Acad Sci U S A, 94(11), pp.5826~5830 (1997)
  51. 전성희, "Nitric Oxide에 의한 혈관내피세포 내 Ca2+ 조절 기전:Cyclic GMP 의존적 및 비의존적 기전.", Korean Circulation Journal, 34(6), pp.600~609 (2004), 대한심장학회
  52. Hwa Jin Lee, "Inhibitory Activity of Medicinal Herbs on Nitric Oxide Synthesis in Activated Macrophages", Natural Product Sciences, 11(1), pp.16~21 (2005), 한국생약학회
  53. Tae Hoon Lee, "Methanol Extracts of Stewartia koreana Inhibit Cyclooxygenase-2 (COX-2) and Inducible Nitric Oxide Synthase (iNOS) Gene Expression by Blocking NF-κB Transactivation in LPS-activated RAW 264.7 Cells", Molecules and Cells, 23(3), pp.398~404 (2007), 한국분자세포생물학회
  54. 김창훈, "蘆薈(알로에), 紫花地丁의 항산화, 항염증, 주름, 미백에 미치는 영향", 한방안이비인후피부과학회지, 23(1), pp.23~43 (2010), 대한한방안이비인후피부과학회
  55. 모정희, "황칠나무 잎 메탄올 추출물 및 분획물의 Tyrosinase 저해 및 Melanin 생성억제 활성", 아시안뷰티화장품학술지, 11(2), pp.275~280 (2013), 한국피부과학연구원
  56. Zolghadri S, "A comprehensive review on tyrosinase inhibitors", J Enzyme Inhib Med Chem, 34(1), pp.279~309 (2019)
  57. 박혜수, "Angelica gigas 에탄올 추출물의 Hyaluronic acid 합성 효과에 대한 실험적 연구", 한방안이비인후피부과학회지, 31(1), pp.32~41 (2018), 대한한방안이비인후피부과학회[DOI]
  58. 안세연, "순채 점액질 다당체 추출물의 피부 보습 효과 연구", 대한화장품학회지, 43(3), pp.223~230 (2017), 사단법인 대한화장품학회
  59. Kligman AM, "Effects of topical tretinoin on non-sunexposed protected skin of the elderly", J AM Acad Dermatol, 29(1), pp.25~33 (1993)
  60. So BH, "Device evaluation of skin effect", The Journal of Skin Barrier Research, 8(1), pp.68~75 (2006)

관련 논문AI추천

이 간행물의 다른 논문 (9건)