발효 균주에 따른 전복발효추출물이 피부 미백 및주름개선에 미치는 영향

Abstract

The effect of fermented abalone hydrolysate by various strains was studied as a raw material for functional cosmetics. Chondroitin sulfate content of fermented extract was compared with normal extract to measure change of active component content. The anti-oxidant effect was researched by using DPPH radical inhibition activity assay. To confirm whitening and anti-wrinkle effects, fermented abalone extract was measured by melanin assay and sicol collagen assay. The cell viability of fermented abalone extract was measured to induce toxicity in NIH-3T3 and B16F10 cells using thiazolyl blue tetrazolium bromide (MTT). Also, subjects were tested on their irritation, elasticity, wrinkle etc. DPPH radical inhibition activity test of fermented abalone extract showed over 80% at 0.01%. It was higher than that of ascorbic acid. The cell viability was confirmed to no toxicity by the MTT assay. Anti-wrinkle effect showed 70% collagen increase at 1% and similar to adenosine (0.04%). Furthermore, fermented abalone extract were showed 30%-35% skin elasticity in clinical test and area of skin wrinkles was improved 20% in fermented abalone extract compared with normal extract. The pores and pophyrin activity were decreased significantly. These results suggest that fermented abalone extract was available as a natural ingredient of cosmeceutical by anti-wrinkle and elasticity effects.