Anti-skin Aging Effect of Pleurotus citrinopileatus Extracts
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To develop a new functional agent for cosmetics, we investigated the anti-aging activities in fibroblasts using Pleurotus citrinopileatus extracts. The antioxidant activities of Pleurotus citrinopileatus extracts (PCE) were determined by using four different methods, namely, DPPH and ABTS radical scavenging assay, ferric reducing antioxidant power (FRAP) assay and oxygen radical absorbance capacity (ORAC) assay. And antioxidant effect of PCE was evaluated by estimating its protective effects against the oxidative stress induced by H2O2 on HDFs. PCE showed radical scavenging and cytoprotective activity against oxidative stress in a dose dependent manner. PCE additionally prevented the elevation of MMP-1 and reduction of Type I collagen (COL1A1) induced by H2O2. Theses finding indicated that PCE protects HDFs from H2O2-induced damage by antioxidant activity and therefore can be used as an anti aging agent.
Abstract
To develop a new functional agent for cosmetics, we investigated the anti-aging activities in fibroblasts using Pleurotus citrinopileatus extracts. The antioxidant activities of Pleurotus citrinopileatus extracts (PCE) were determined by using four different methods, namely, DPPH and ABTS radical scavenging assay, ferric reducing antioxidant power (FRAP) assay and oxygen radical absorbance capacity (ORAC) assay. And antioxidant effect of PCE was evaluated by estimating its protective effects against the oxidative stress induced by H2O2 on HDFs. PCE showed radical scavenging and cytoprotective activity against oxidative stress in a dose dependent manner. PCE additionally prevented the elevation of MMP-1 and reduction of Type I collagen (COL1A1) induced by H2O2. Theses finding indicated that PCE protects HDFs from H2O2-induced damage by antioxidant activity and therefore can be used as an anti aging agent.
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