싸리꽃 에탄올 추출물의 皮膚 生理活性 효과
Dermal Bioactive Properties of the Ethanol Extract from Flowers of Lespedeza bicolor
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Abstract
Objective:In this study, the ethanol extract from flowers of Lespedeza bicolor was investigated for their dermal bioactive properties related to cosmeceuticals such as depigmentation and radical scavenging effect. Results:The ethanol extract from flowers of Lespedeza bicolor showed considerable radical scavenging activity (SC50: 10 ㎍/㎖) and inhibited the production of nitric oxide (NO) in Raw 264.7 macrophages activated with the endotoxin lipopolysaccharide (LPS). Although the proliferation of B16/F10 cells was slightly decreased by the ethanol extract from flowers of Lespedeza bicolor at the concentration of 100 ㎍/㎖, it did not appear necrosis. The ethanol extract from flowers of Lespedeza bicolor down-regulated melanin formation effectively. Methods:The free radical scavenging activity of Lespedeza bicolor was assayed in cell free systems using a stable free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH). Nitrite accumulated in culture medium was measured as an indicator of NO production using a Griess reaction. Cell viability was measured by MTT assay and melanin content was assessed using the method of Hosei with some modifications. Conclusions:These results suggest that the ethanol extract from flowers of Lespedeza bicolor is a potent depigmetation agent and it may be a candidate for antioxidant and anti-inflammatory agent.
참고문헌 (25)
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