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加味大黃膏의 멜라닌세포 활성억제가 자외선 조사로 인한 피부 손상 완화에 미치는 영향

The effect of Daehwanggogasangbakpi(大黃膏加桑白皮) on the skin damage induced by ultraviolet irradiation

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대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2007.08|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제20권 제2호|pp. 47-67

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주제분류한의학

초록

Abstract

Objective As a result of increasing amount of ultraviolet ray, skin problems including sunburn, rapid skin aging, melanoma, and even skin cancer continue to rise. In the present study, the effect of oriental herbal extract, Daehwanggo(大黃膏,DH) and Daehwanggogasnagbakpi(大黃膏加桑白皮,DS), as external application, on the skin damage, was investigated. Methods 30 mice were equally distributed into 3 groups : control, UVB-control and UVB-irradiated and DS-treated group. Also mouse melanoma cell lines were cultured. Tyrosinase inhibition was measured to analyze the UV-protection effect. Melanogenesis in the UV-irradiated melanoma cell lines was compared in DS-treated cell line and control cell line. Sample skin from the ear tissue of the 3 groups were analyzed to observe the inflammatory response, T cell differentiation, apoptosis of keratinocytes. Results The tyrosinase was more significantly inhibited in the DS group compared to DH group. Antioxidative effects was more prominent in DS group when superoxide dismutase was measured. Both the DS- and DH-treated cell lines showed significantly reduced melanogenesis. The reduction of external skin damage including erythematous papule, eczema, keratinocyte, pyopoiesis was observed in the DS- and DH-treated sample cells. In terms of the effect on the skin damage, sunburn cell, activated skin mast cells, secretion of IL-12, manifestation of HSP70, hyperplasia of epithelial cells, MMP-9 and destruction of the collagen were all significantly improved in the DS-treated sample cells. Melanin cells and the apoptosis in the melanoma cell line were decreased. Conclusion DH and DS were traditionally applied externally for the scald in the oriental medicine. The present study elucidated the possibility of herbal extracts to be used as ultraviolet protectives. Further investigations are needed to assure the clinical application.

참고문헌 (66)

  1. "생활 속의 자외선", pp.15~30 (2003), 서울:화장품신문사
  2. "M. A. Sunscreen and their use in the preventive treatment of sunlight induced skin damage", pp.739~50 (1987)
  3. "상백피 추출물의 멜라닌 생성 저해효과", 12, pp.327~336 (1998), 서울산업대학교
  4. "자외선 차단 및 미백 효과를 지니는 생약 추출물의 검색" (2004)
  5. "자외선흡수제로서의 식물추출성분의 안정성과 효과" (2000)
  6. "산뽕나무 뿌리 추출물의 자유라디칼 소거 및 자외선 흡수효과에 관한 연구" (2004)
  7. "부위별 산뽕나무의 광보호 효과 및 항산화 활성", 35(3), pp.207~214 (2004)
  8. "녹차추출물 성분 Catechin이 자외선에 의해 손상된 피부 및 Apoptosis에 미치는 영향" (2001)
  9. "마우스에서 녹차 추출물의 자외선 피부손상 경감효과에 관한 형태학적 연구" (2006)
  10. "민용태. 보중익기탕의 투여가 자외선 조사로 저하된 마우스의 면역 기능의 회복에 미치는 영향. 원광대학교 대학원 한의학과 박사논문. 1991."
  11. "Pigment production in murine melanoma cell is regulated by tyrosinase", pp.126~131
  12. "Stimulation of melanogenesis in human melanoma cell line by retinoids. Cancer Res. 1980", pp.3345~52
  13. "The nature of photoaging", pp.215~27
  14. "Fitzpatrick TB. Preventive treatment of sunburn and skin cancer with sun-protective agents. In et al. eds. Dermatology in general medicine", pp.1658~60, McGraw-Hill Inc. 1993
  15. "자외선 B조사 및 수종의 자외선 차단제가 Hairless Albino Mouse의 피부노화에 미치는 영향", 34(2), pp.289~299 (1996)
  16. "Implications of an Education Intervention Program Designed to Increse Young Adolescents Awareness of Hats for Sun Protection", 17(2) (1999)
  17. "한방화장품의 현황과 발전방향에 관한 고찰", pp.52~60 (2004), 서울대학교 대학원
  18. "도깨비부채 뿌리 추출물의 멜라닌 생성억제효과", 14(1), pp.27~30 (2006)
  19. "A clinical study on compound da huang (radix et rhizoma rhei) preparations for improvement odsennile persons' memory ability", 17(3), pp.168~173 (1997)
  20. "Direct scavenging of nitric oxide by traditional crude drugs", 6(6), pp.453~463 (2000)
  21. "대황, 유향, 몰약의 도포가 화상 피부 치료에 미치는 영향" (2001)
  22. "유병수. 참당귀근의 Coumarin성분에 관한 연구. 약학회지. 1967", pp.22~
  23. "Preventive effects of traditional chinese(Kampo) medicines on experimental osteoporosis induced by ovariectomy in rats", 61, pp.239~246 (1997)
  24. "황백열수추출물의 항산화활성과 아질산염 소거작용에 관한 연구", 31(1), pp.112~118 (1999)
  25. "Effects of different processing products of Cortex Phellodendri on scavenging oxygen free radicals and anti-lipid peroxidation", 26, pp.245~248 (2001)
  26. "日本藥局方解說書 第十三改正", pp.221~448 (1996), 東京, 廣川書店
  27. "a new flavone derivatives from the root barks of the cultivated mulberry tree", kuwa(n g), pp.2548~
  28. "Constituents of the cultivated mulberry tree. XXV. Structures of two natura hypotensive Diels-Alder type adducts mulberrofurans F and G from the cultivated mulberry tree", pp.3195~
  29. "Cytarabine으로 유도된 탈모증에 대한 상백피 추출물의 육모촉진 효과", 44(1), pp.66~70 (2000)
  30. "Lozano JA. Regulation of mammalian melanogenesis. the role of dopachrome tautomerase and the ratio between 5", pp.6 155~63
  31. "et al. Molecular casade in UV-induced melanogenesis. Pigment Cell Res 1992", pp.348~56
  32. "Oxyreseveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyorsinase", 243(3), pp.801~803 (1998)
  33. "Mulberroside F isolated from the leaves of Morus alba inhibits melanin synthesis", 25, pp.1045~1048 (2002)
  34. "Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells", 33, pp.131~135 (2001)
  35. "inhibition of tyrosinase by green tea components", 65, pp.241~246 (1999)
  36. "정상 인체 멜라닌세포 및 B-16 흑색종 세포주에서 상지추출물의 티로시나제 활성 억제 효과에 관한 연구", 6(2), pp.391~400 (2000)
  37. "인삼의 부위별 페놀성 성분 함량 및 멜라닌 생성억제효과", 14(3), pp.148~152 (2006)
  38. "소목의 에틸아세테이트 추출물이 B16", F10 흑색종세포의 멜라닌 합성에 미치는 영향, 2001, pp.15(6), 961~966
  39. "유기노의 항산화 활성 및 멜라닌 생성 억제 효과", 36(4), pp.273~277 (2005)
  40. "B16 melanoma 세포주의 멜라닌 합성에 대한 서시옥용산의 효과", 14(1), pp.160~170 (2000)
  41. "Mishima Y. Tyrosinase gene transcription and its control by melanogenic inhibitors. J Invest Dermatol. 1993", pp.2 150~5
  42. "Fridovich I. Free radicals in cutaneous biology. J invest Dermatol 1994", pp.671~5
  43. "Repeated exposure of human skin fibroblasts to UVB at subcyto-toxic level triggers premature sensecence through the TGF-betal signaling pathway", 118, pp.743~758 (2005)
  44. "Effects of ultraviolet A and antioxidant defense in cultured fibroblasts and Keratinocytes", 11, pp.192~197 (1996)
  45. "Increased oxydative stress in sciatic nerves of streptozotocin induced diabetic rat: Lack of vitamin C effect", 19(3), pp.279~286 (1995)
  46. "Grape seed proanthocyanidins inhibit UV-radiation-induced oxidative stress and activation of MAPK and NF-kappaB signaling in human epidermal keratinocytes", 40(9), pp.1603~1614 (2006)
  47. "Antioxidant defense mechanisms in murine epidermis and dermis and their responses to ultraviolet light. J Invest Dermatol. 1993", pp.260~5
  48. "배양된 섬유모세포에서 자외선 B 조사 시 발현 변화를 보이는 유전자군에 멜라토닌이 미치는 영향", 44(3), pp.257~266 (2006)
  49. "산뽕나무 뿌리 추출물의 자유라디칼 소거 및 자외선 흡수효과에 관한 연구" (2004)
  50. "Recent advances in the chemistry of melanogenesis in mammals. J. Invest. Dermatol. 1980", pp.122~8
  51. "Cyclic AMP a key messenger in the regulation of skin pigmentation", 13(2), pp.60~69 (2000)
  52. "Induction of melanogenesis suppression cellular pharmacology and mode of differential action. Pigment Cell Res. 1988", pp.367~74
  53. "Epidermal stratification reduces the effects of UVB (but not UVA) on keratinocyte cytokine production and cytotoxicity", 13(4), pp.147~152 (1997)
  54. "Regulation of TNFalpha production and release in human and mouse keratinocytes and mouse skin after UV-B irradiation", 16(6), pp.263~270 (2000)
  55. "Deficient Th1-type immune responses via impaired CD28 signaling in ultraviolet B-induced systemic immunosuppression and the restorative effect of IL-12", 24(3), pp.190~202 (2000)
  56. "Orally administered green tea polyphenols prevent ultraviolet radiation-induced skin cancer in mice through activation of cytotoxic T cells and inhibition of angiogenesis in tumors", 135(12), pp.2871~2877 (2005)
  57. "In vitro and In vivo Inhibition of Skin Matrix Metalloproteinases by Pothomorphe umbellata Root Extract" (2005)
  58. "Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events", 973, pp.31~43 (2002)
  59. "The heat-shock response", 2, pp.198~207 (1995)
  60. "Heat shock protein as markers of neuronal injury. Mol. Brain Res. 1989", pp.93~100
  61. "Induction of stress protein HSP70 in nerve cell after status epilepticus in the rat. Neurosci Lett. 1989", pp.259~264
  62. "Initiation of protein synthesis and heat shock protein-72 expression in the rat brain following sereve insulin induced hypoglycemia. Acta Neuropathol. 1989", pp.259~64
  63. "Hsp 70 - Nuclear concentration during enviromental stress and cytoplasmic stroage during recovery. Cell. 1984", pp.622~55
  64. "Molecular regulation of UVB-induced cutaneous angiogenesis", 111(5), pp.864~872 (1998)
  65. "Prevention of the ultraviolet B-mediated skin photoaging by a nuclear factor kappaB inhibitor, parthenolide", 315(2), pp.624~630 (2005)
  66. "Human keratinocytes maintain reversible anti-apoptotic defenses in vivo and in vitro", 2(2), pp.136~148 (1997)

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