역학적 강도 분석을 이용한 저강도 초음파의 조사 부위의 골다공증 골절 방지 효과 평가
In vivo Evaluation of Osteoporotic Fracture Prevention of the site to which low Intensity Ultrasound is Irradiated using Mechanical Strength Simulations
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Purpose: The aim of present study is to evaluate a possibility of clinical application for the effect of low intensity ultrasound stimulation (LIUS) in mechanical characteristics of bone on osteoporotic fractures prevention. Materials and Methods: Eight virgin ICR mice (14 weeks old, approximate weight 25g) were ovariectomized (OVX) to induce osteoporosis. The right hind limbs were then stimulated with LIUS (US Group), whereas left hind limbs were not stimulated (CON Group). Both hind limbs of all mice were scanned by in-vivo micro-CT to acquire two-dimensional (2D) images at 0 week before stimulation and 3 weeks and 6 weeks after stimulation. Three-dimensional (3D) finite element (FE) models generated by scanned 2D images were used to determine quantitatively the effect of LIUS on strength related to bone structure. Additionally, distributions of Hounsfield units and elastic moduli, which are related to the bone quality, for the bones in the US and CON groups were determined to analyze quantitatively a degree of improvement of bone qualities achieved by LIUS. Results: The result of FE analysis showed that the structural strength in US Group was significantly increased over time (p＜0.05), while that in CON Group was statistically constant over time (p＞0.05). High values of Hounsfield units obtained from voxels on micro-CT images and high values of elastic moduli converted from the Hounsfield units were dominantly appeared in US Group compared with those in CON Group. Conclusion: These finding indicated that LIUS would improve the mechanical characteristics of osteoporotic bone via the effects of bone structure (bone strength) and quality (Hounsfield unit and elastic modulus). Therefore, the LIUS may decrease effectively the risk of osteoporotic fracture in clinics.
Abstract
Purpose: The aim of present study is to evaluate a possibility of clinical application for the effect of low intensity ultrasound stimulation (LIUS) in mechanical characteristics of bone on osteoporotic fractures prevention. Materials and Methods: Eight virgin ICR mice (14 weeks old, approximate weight 25g) were ovariectomized (OVX) to induce osteoporosis. The right hind limbs were then stimulated with LIUS (US Group), whereas left hind limbs were not stimulated (CON Group). Both hind limbs of all mice were scanned by in-vivo micro-CT to acquire two-dimensional (2D) images at 0 week before stimulation and 3 weeks and 6 weeks after stimulation. Three-dimensional (3D) finite element (FE) models generated by scanned 2D images were used to determine quantitatively the effect of LIUS on strength related to bone structure. Additionally, distributions of Hounsfield units and elastic moduli, which are related to the bone quality, for the bones in the US and CON groups were determined to analyze quantitatively a degree of improvement of bone qualities achieved by LIUS. Results: The result of FE analysis showed that the structural strength in US Group was significantly increased over time (p＜0.05), while that in CON Group was statistically constant over time (p＞0.05). High values of Hounsfield units obtained from voxels on micro-CT images and high values of elastic moduli converted from the Hounsfield units were dominantly appeared in US Group compared with those in CON Group. Conclusion: These finding indicated that LIUS would improve the mechanical characteristics of osteoporotic bone via the effects of bone structure (bone strength) and quality (Hounsfield unit and elastic modulus). Therefore, the LIUS may decrease effectively the risk of osteoporotic fracture in clinics.
참고문헌 (17)
- Carvalho DCL, "The action of low-intensity pulsed ultrasound in bones of osteopenic rats", Artificial Organs, 28, pp.114~118 (2004)
- Duarte LR, "The stimulation of bone growth by ultrasound", Arch Orthop Trauma Surg, 101, pp.153~159 (1983)
- Heckman JD, "Acceleration of tibial fracture-healing by non-invasive, low-intensity, pulsed ultrasound", Journal of Bone Joint Surg, 76A, pp.226~234 (1994), 226-234
- Kang JD, "Case-control study of risk factors of fracture due to osteoporosis", The Journal of the Korean Society of Fractures, 15, pp.8~ (2002)
- 김형준, "골다공증 진단을 위한 초음파 영상화 진단 기법 연구", 비파괴검사학회지, 22(4), pp.386~392 (2002), 한국비파괴검사학회
- Knoch HG, "Stimulation of Fracture Healing with Ultrasound", Springer-Verlag, 1, pp.1~87 (1990)
- 고창용, "난소 절제술을 시행한 흰쥐 등뼈의 해면골과 피질골의 구조적 특성 추적관찰 및 기계적 특성 분석-생체 내 미세단층촬영 시스템과 미세유한 요소법 적용-", 한국정밀공학회지, 24(1), pp.110~117 (2007), 한국정밀공학회
- Lee HY, "A Study of Correlation among the Knowledge of the Disease Health Promoting Behaviors and the Quality of Life in the Female Patients with Osteoporosis", The Journal of Rheumat- ology Health, 8, pp.68~ (2001)
- Lee JY, "Osteoporosis", Human science, 14(4), pp.473~479 (1990)
- Pilla AA, "Non-invasivelow intensity pulsed ultrasound accelerates bone healing in the rabbit", J Orthop Trauma, 4, pp.246~253 (1990)
- Rantanen J, "Effects of therapeutic ultrasound on the regeneration of skeletal myofibers after experimental muscle injury", American Journal of Sports Medicine, 27, pp.54~59 (1999)
- Rho JY, "Relations of Mechanical Properties to Density and CT Numbers in Human Bone", Medical Engineering and Physics, 17, pp.347~355 (1995)
- Rubin CD, "Osteoporosis: Considerations in evaluation and managing older patients", Texas Medicine, 89, pp.67~74 (1993)
- "The Korean Society of Bone Metabolism: Physician's guideline for osteoporosis", Seoheung, ~ (2007)
- Warden SJ, "Skeletal effects of low-intensity pulsed ultrasound on the ovariectomized rodent", Ultrasound in Medicine and Biology, 27, pp.989~998 (2001)
- Wiltink A, "Effect of therapeutic ultrasound on endochondral ossification", Ultrasound Med Biology, 21, pp.121~127 (1995)
- Yang KH, "Stimulation of fracture Healing by Low-Intensity Pulsing Ultrasound", The Journal of the Korean Society of Fractures, 11, pp.248~ (1998)