본문 바로가기
KERD
논문KCI 등재

전정신경핵에서 수평 반규관의 선택적 자극에 의한 pERK1/2와 cFos 단백 발현

Expression of Phosphorylated ERK1/2 and cFos Proteins in Vestibular Nuclei by Selective Stimulation of Horizontal Semicircular Canal

조광호|최명애|이학승|이영주|박성욱|이재효|김민선|박현영|박병림|김요식|장혁|최봉기
대한신경과학회 (Korean Neurological Association)|2005.04|대한신경과학회지 (Journal of the Korean Neurological Association)|제23권 제2호|pp. 257-263

0

조회

0

다운로드

0

인용

0

참고문헌

주제분류신경과학

초록

Abstract

Background: There is a little information about the effect of selective vestibular stimulation on the expression of activity-dependent metabolic markers in the vestibular nuclei. The purpose of this study was to evaluate effect of afferent excitation of the horizontal semicircular canal on expression of phosphorylated ERK1/2 (pERK1/2) and cFos proteins in the vestibular nuclei. Methods: The horizontal semicircular canal of Sprague-Dawley rats was selectively stimulated by using the sinusoidal horizontal stimulator with 10-minute duration of stimulation. Conventional immunohistochemical method was used to visualize pERK1/2 or cFos immunoreactive neurons in the vestibular nuclei following rotation. Results: Five minutes after stimulation of the horizontal semicircular canal there was a high expression of pERK1/2 protein in the medial vestibular nucleus among 4 major subnuclei of the central vestibular nuclear complex. On the contrary, immunoreactivity of cFos protein was observed in the medial and inferior vestibular nucleus 2 hours after horizontal sinusoidal rotation. The lateral vestibular nucleus was free from the expression of pERK1/2 and cFos proteins in response to excitation of the horizontal semicircular canal. However, in the vestibular nuclei of unilaterally labyrinthectomized rats expression of pERK and cFos proteins was markedly suppressed in ipsi-lesional side as well as contra-lesional side following stimulation of the horizontal semicircular canal. Furthermore no expression of pERK1/2 and cFos protein in the bilateral vestibular nuclei of bilaterally labyrinthectomized rats was noted after stimulation of the horizontal semicircular canal. Conclusions: Therefore these results of present study suggest that excitatory afferent signals from the peripheral vestibular receptors are essential for protein translation for pERK1/2 and cFos in response to stimulation of the semicircular canal.

참고문헌 (30)

  1. "Spatial organization of neck and vestibular reflexes acting on the forelimbs of the decerebrate cat", 55, pp.514~526 (1986)
  2. "The neural pathways mediating reflex contraction of extraocular muscles during semicircular canal stimulation in rabbit", 55, pp.183~188 (1973)
  3. "Studies on the interrelationship between the vestibular semicircular canals and the extraocular muscles in rabbits and cats", 21, pp.91~101 (1987)
  4. "Stimulus-transcription coupling in the nervous system:involvement of the inducible proto-oncogenes fos and jun", 14, pp.421~451 (1991)
  5. "Translabyrinth electrical stimulation for the induction of immediate-early genes in the gerbil brainstem", 646, pp.345~350 (1994)
  6. "c-fos Expression in the rat brain after unilateral labyrinthectomy and its relation to the uncompensated and compensated stage", 70, pp.515~546 (1996)
  7. "Effect of MK801 on cFos-like protein expression in the medial vestibular nucleus at early stage of vestibular compensation in uvulonodulectomized rats", 231, pp.147~150 (1997)
  8. "Extracellular signal-regulated kinase signalling in neurons", 9, pp.544~553 (1999)
  9. "Is the Ras-MAPK signalling pathway necessary for lon-term memory formation", pp.38~44 (1999)
  10. "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras", 12, pp.1207~1221 (1994)
  11. "Cross talk between ERK and PKA is required for Ca2+ stimulation of CREB-dependent transcription and ERK nuclear translocation", 21, pp.869~883 (1998)
  12. "Extracellular signal-regulated kinase(ERK)controls immediate early gene induction on corticostriatal stimulation", 18, pp.8814~8825 (1998)
  13. "Nuclear localization and regulation of erk and rsk-encoded protein kinases", 12, pp.915~927 (1992)
  14. "ERK phosphorylation potentiates Elk-1 mediated ternary complex formation and transactivation", 14, pp.951~962 (1995)
  15. "Protein synthesis inhibitors revel differential regulation of mitogen-activated protein kinase and stress-activated protein kinase pathways that converge on Elk-1", 15, pp.4930~4938 (1995)
  16. "In vivo expression and regulation of Elk-1,a target of the extracellular-regulated kinase signaling pathway,in the adult rat brain", 18, pp.214~226 (1998)
  17. "Glutamate induces phosphorylation of Elk-1 and CREB,along with c-fos activation,via an extracellular signal-regulated kinase-dependent pathway in brain slices", 19, pp.136~146 (1999)
  18. "Immunohistochemical Identification of Phosphorylated Extracellular Signal-Regulated Kinase1", 2 in Rat Vestibular Nuclei by Unilateral Labyrinthectomy, 2003, pp.2, 170~174
  19. "A study of the sinusoidal head rotator", 25, pp.25~30 (1988)
  20. "The Rat Brain in stereotaxic coordinates", paxi(s g) (1998), Academic Press
  21. "Projection to the spinal cord from the medial and descending vetibular nuclei of the cat", 215, pp.429~430 (1967)
  22. "Fos-defined activity in rat brainstem following centripetal acceleration", 12, pp.4489~4500 (1992)
  23. "Effects of acute hypotension on expression of cFos-like protein in the vestibular nuclei of rats", 962, pp.111~121 (2003)
  24. "Immunohistochemical detection of phosphorylated form of extracellular signal-regulated kinase 1", 2 in rat bestibular nuclei followin hemorrhagic hypotension, 2004, pp.360, 49~52
  25. "Brainstem Fos expression following acute unilateral labyrinthectomy in the rat", 3, pp.829~832 (1992)
  26. "Temporal changes of cFos-like protein expression in medial vestibular nuclei following arsanilate-induced unilateral labyrinthectomy in rats", 319, pp.9~12 (2002)
  27. "Effects of acute hypotension on neuronal activity in the medial vestibular nuclei of rats", 12, pp.3821~3824 (2001)
  28. "Neuronal activity in the ipsilateral medial vestibular nucleus of the guinea pig following unilateral labyrinthectomy", 444, pp.308~319 (1988)
  29. "Vestibular compensation:a review of the oculomotor,neural,and clinical consequences of unilateral vestibular loss", 5, pp.67~107 (1995)
  30. "Compensation of horizontal canal related activity in the medical vestibular nucleus following unilateral labyrinth ablation in the decerebrate gerbil", pp.359~372

관련 논문AI추천

흰쥐 전정신경핵에서 전하소뇌동맥의 일시적 폐쇄에 의한 급성초기유전자 단백의 발현장혁, 김민선, 김요식, 박병림, 박성욱, 박현영, 이승회, 이한샘, 조광호, 최명애, 한형석대한신경과학회 · 대한신경과학회지2004 · 제22권 제4호흰쥐 내측 전정핵 뉴론에서 Norepinephrine의 포타슘 전류 억제 효과황매, 김병채, 박종성, 장명주, 정한성, 조기현, 허해룡대한신경과학회 · 대한신경과학회지2005 · 제23권 제3호내측전정핵 뉴론 안정 활성도 증가에 미치는5-Hydroxytryptamine의 작용허탁, 황매, 김자연, 김재하, 박종성, 장명주, 정한성, 조용범대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제9호전정 신경핵 칼륨 전류에 미치는 5-Hydroxytryptamine의 영향박종성, 장명주, 전제열, 정한성, 조용범, 차선화대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제11호표피 전기 자극에 의한 전정 신경의 반응：내이 전기 자극에 의한 반응과의 비교박홍주대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2005 · 제48권 제7호흰쥐 내측 전정핵세포의 막전도도와 시냅스 전달에 대한 Orphan Opioid의 작용장혁, 박병림, 박현영, 이문영, 조광호, 천상우, 최정희대한신경과학회 · 대한신경과학회지2004 · 제22권 제6호

이 간행물의 다른 논문 (14건)

급성 중대뇌동맥 경색 후 발생하는 행동심리적 증상최성민, 이승한, 박만석, 김병채, 김명규, 조기현, 남태승, 박종귀pp. 152-157뇌경색 아형군의 재발 기전: 추적 관찰 연구신동훈, 방오영pp. 158-164M-mode TCD를 이용한 한 중대뇌동맥의 양측 측두창을 통한 동시 탐지석승한, 양현덕, 손일홍pp. 172-175만성 긴장형두통 및 편두통 환자들에서 세로토닌 수송체 다형성과 위해 회피 인성차원김영인, 이광수, 김중석, 박정욱pp. 181-185편두통 및 긴장형두통 환자의 심리사회적 특성이동국, 최소영, 박권생, 도진국pp. 192-198
원문 보기