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논문KCI 등재

흰쥐 내측 전정핵세포의 막전도도와 시냅스 전달에 대한 Orphan Opioid의 작용

Actions of Orphan Opioid on the Membrane Conductances and Synaptic Transmission in Rat Medial Vestibular Nucleus Neurons

조광호|천상우|최정희|이문영|박병림|박현영|장혁
대한신경과학회 (Korean Neurological Association)|2004.12|대한신경과학회지 (Journal of the Korean Neurological Association)|제22권 제6호|pp. 631-637

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참고문헌

주제분류신경과학

초록

Abstract

Background: The medial vestibular nucleus (MVN) is involved in the reflex control of the head and eyes, and the recovery of vestibular function after vestibular injuries. This study was performed to investigate the actions of the orphan opioid (nociceptin) on the membrane conductances and synaptic transmission in rat MVN neurons. Methods: Whole cell patch clamp recordings were carried out in the brainstem slice of neonatal rats. Results: Nociceptin (2 µM) inhibited the spontaneous discharge in the majority (83%) of MVN neurons. This inhibition was insensitive to the non-specific opioid receptor antagonist naloxone (10 µM), but was effectively antagonized by the selective opioid receptor-like 1 (ORL1) receptor antagonist, [Nphe1] nociceptin(1-13)NH2 (3 µM). Nociceptin had no effect on the rate or amplitude of miniature inhibitory postsynaptic currents (mIPSCs). Nociceptin induced an outward current, and which was blocked by [Nphe1] nociceptin(1-13)NH2 in MVN neurons. Outward current reversed at -81±2 mV, which was close to the K＋ equilibrium potential as calculated by the Nernst equation in 6 mM extracellular potassium solution. This indicates that the action of nociceptin involves postsynaptic receptors on the MVN neurons. Conclusions: These results suggest that nociceptin modulate neuronal excitability by activating a K＋ conductance in postsynaptic neurons, not by modulation of synaptic transmission in MVN neurons.

참고문헌 (33)

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