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흰쥐 전정신경핵에서 전하소뇌동맥의 일시적 폐쇄에 의한 급성초기유전자 단백의 발현

Expression of Immediate-Early Gene Proteins by Transient Occlusion of Anterior Inferior Cerebellar Artery in Vestibular Nuclei of Sprague-Dawley Rats

조광호|한형석|김민선|박성욱|최명애|박현영|박병림|김요식|장혁|이한샘|이승회
대한신경과학회 (Korean Neurological Association)|2004.08|대한신경과학회지 (Journal of the Korean Neurological Association)|제22권 제4호|pp. 360-367

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참고문헌

주제분류신경과학

초록

Abstract

Background: Anterior cerebellar artery (AICA) occlusion results in vestibular dysfunctions because the AICA supplies the vestibular nuclei (VN) in the brain stem as well as the peripheral vestibular organs in the inner ear. The purpose of this study was to evaluate the expression of immediate-early gene products, a metabolic marker of neural excitation in neurons, by AICA occlusion in the VN of Sprague-Dawley rats. Methods: After chloral hydrate anesthesia all animals were subjected to unilateral AICA occlusion by using a microsurgical clamp for 30 min to induce a transient ischemia. Unilateral labyrinthectomy was chemically undertaken to eliminate vestibular afferent activity. Immunohistochemical staining and image analysis for cFos, FosB, Krox-24, and JunB proteins were performed 2 hours after AICA occlusion. Results: There was a high expression of cFos protein in the bilateral medial and inferior VN 2 hours after AICA occlusion. AICA occlusion induced minimal changes in cFos protein expression in the lateral and superior VN. Mild to moderate expressions of FosB and JunB protein in VN was observed 2 hours after ischemic injury of the brain stem and inner ear. On the contrary, the number of cFos and FosB immunoreactive neurons significantly decreased in the medial vestibular nucleus ipsilateral to the injured labyrinth 2 hours after AICA occlusion in the UL group. Conclusions: These results suggest that ischemic afferent activity from the peripheral vestibular apparatus is essential for the expression of immediate-early gene products in the medial and inferior VN of rats following AICA occlusion.

참고문헌 (34)

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