S-nitroso-N-acetylpenicillamine이 내측 전정핵 신경세포 흥분도에 미치는 영향
Effects of S-nitroso-N-acetylpenicillamine on the Neuronal Excitability of the Medial Vestibular Nuclear Neuron
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Abstract
Background: The medial vestibular nucleus is the largest one among the vestibular nuclei and known to play important roles not only in normal vestibular information processing but also in vestibular compensation. Glutamate is known to have a key role in vestibular compensation via long term potentiation and depression. But the action of nitric oxide related with glutamate is poorly studied. This experiment was designed to explore the effects of nitric oxide on the neuronal activity of a rat medial vestibular nuclear neuron using a nitric oxide enhancing drug, S-nitroso-N-acetylpenicillamine (SNAP). Methods: Experiments were carried out on Sprague-Dawley rats aged 14 to 17 days. Neurons of MVN were obtained via enzymatic dissociation of a microtomized rat brainstem. Whole-cell membrane potentials were recorded at room temperature by using standard patch-clamp techniques. Action potentials were obtained after administration of SNAP. Changes of potassium currents were recorded using SNAP and ODQ (1H-[1, 2, 4] oxadiazolo [4, 3-a] quinozalin-1-one), an inhibitor of guanylyl cyclase. Results: The mean spike frequency of action potentials was increased by adding SNAP. The mean amplitude of afterhyperpolarization was decreased by adding SNAP. The mean potassium current of medial vestibular nuclear neurons was decreased by SNAP. ODQ inhibited the SNAP-induced potassium currents. Conclusions: These results suggest that nitric oxide increases the neuronal activity of rat medial vestibular nuclear neurons by inhibiting potassium currents via a cGMP dependent mechanism.
참고문헌 (35)
- "Mountcasie VB. Handbook of Physiology. 3rd. Maryland", pp.980~
- "Scher AM and Steiner RL. Textbook of physiology", pp.582~ (1989)
- "Hiranuma K. Axonal trajectories of inhibitory vestibulocollic neurons activated by the anterior semicircular canal nerve and their synaptic effects on neck motoneurons in the cat. Exp Brain Res 1990", uchi(y), pp.14~24
- "Muhlethaler M. Medial vestibular nucleus in the guinea pig Intrinsic membrane properties in brainstem slices. Exp Brain Res 1991", pp.417~425
- "Snyder SH. A novel neuronal messenger molecule in the brain", pp.297~311
- "Nitric oxide as modulator of neuronal function", 64, pp.51~68 (2001)
- "Bruning G. Localization of NADPH-diaphorase in the brain of the chicken. J Comp Neurol 1993"
- "Mayer B. Nitric oxide synthase in the brain of the turtle Pseudemys scripta elegans. J Comp Neurol 1994"
- "Localisation of the nitric oxide (NO)", cGMP-pathway in the vestibular system of guniea pigs, 1998, pp.251, 185~188
- "Effects of nitric oxide on the vestibular functional recovery after unilateral labyrinthectomy", 84, pp.425~430 (2000)
- "Nitric oxide in the flocculus works the inhibitory neural circuits after unilateral labyrinthectomy", 815, pp.405~409 (1999)
- "et. al. Altered synaptic plasticity and memory formation in nitric oxide synthase inhibitor-treated rats. Proc Natl Acad Sci USA 1993", pp.9191~9194
- "Rose SP. Inhibiting synthesis of putative retrograde messenger nitric oxide results in amnesia in a passive avoidance task in the chick. Brain Res 1993"
- "Baader SL. Nitric oxide synthase expression reveals compartments of cerebellar granule cells and suggests a role of mossy fibers in their development. Neuroscience 1994", pp.893~903
- "Synaptic plasticity in the medial vestibular nuclei; role of glutamate receptors and retrograde messengers in rat brainstem slices", 64, pp.527~553 (2001)
- "Wong RK. Isolation of neurons suitable for patch- clamping from adult mammalian central nervous system. J Neurosci Methods 1986", pp.227~238
- "Sigworth FJ. Improved patch clamp techniques for high resolution current recording from cells and cell free membrane patches. Pflugers Arch 1981", pp.85~100
- "Lapetina EG. Activation of a cytosolic ADP-ribosyltransferase by nitric oxide-generating agents. J Biol Chem 1989", pp.8455~8458
- "Gebhart GF. Production of endogenous nitric oxide andactivation of soluble guanylate cyclase are required for N-methyl-D- aspartate-produced facilitation of the nociceptive tail-flick reflex. Eur J Pharmacol 1992", pp.93~96
- "A Berthoz and Melvill Jones G. Adaptive Mechanisms in Gaze Control Facts and Theories. Amsterdam", pp.251~268 (1985)
- "an in vivo and in vitro study of second order vestibular neurons. Soc Neurosci Abstract 1988", pp.331~
- "Vidal PP. NMDA receptors contribute to the resting discharge of vestibular neurons in the normal and hemilabyrinthectomized guinea pig. Exp Brain Res 1990", pp.125~133
- "Darlington CL. Neurochemical mechanisms of recovery from peripheral vestibular lesions", pp.117~133
- "Precht W. Vestibular nuclear neuron activity in chronically hemilabyrinthectomized cats. Acta Otolaryngol 1984", pp.1~13
- "Lannou J. Dynamic characteristics of vestibular nuclear neurons responses to vestibular and optokinetic stimulation during vestibular compensation in the rat. Acta Otolaryngol Suppl 1987", pp.1~19
- Smith PF, "Curthoys IS. Neuronal activity in the ipsilateral medial vestibular nucleus of the guinea pig following unilateral labyrinthectomy. Brain Res 1988", pp.308~319
- Newlands SD, "Perachio AA. Compensation of horizontal canal related activity in the medial vestibular nucleus following unilateral labyrinth ablation in the decerebrate gerbil Type 1 neurons. Exp Brain Res 1990", pp.359~372
- Borel L, "Ouaknine M. Recovery of head postural control following unilateral vestibular neuroectomy in the cat.Neck muscle activity and neuronal correlates in Deiter's nuclei. Acta Otolaryngol", zen(u-azogui y), pp.1~19 (1993)
- Darlington CL, "The recovery of static vestibular function following peripheral vestibular lesions in mammals: the intrinsic mechanism hypothesis", 6, pp.185~201 (1996)
- Gamkrelidze GN, "Role of K+ currents in excitability of developing chick vestibular sensory neurons", 23, pp.751~ (1997)
- Peusner KD, "Potassium currents and excitability in second-order auditory and vestibular neurons", 53, pp.511~520 (1998)
- deWaele C, "Muhlethaler M. Medial vestibular nucleus in the guinea-pig Apamin-induced rhythmic burst firing-an in vitro and in vivo study. Exp Brain Res 1993", pp.213~222
- Johnston AR, "Dutia MS. Ionic conductances contributing to spike repolarization and after-potential in rat medial vestibular nucleus neurons. J Physiol Lond 1994", pp.61~77
- duLac S, "Candidate cellular mechanisms of vestibulo-ocular reflex plasticity", 781, pp.489~498 (1996)
- "Peusner KD. The differential expression of low threshold sustained potassium current contributes to the distinct firing patterns in embryonic central vestibular neurons. J Neurosci 1985", pp.1449~1464
