백리향 (Thymus quinquecostatus Celak.)의 기내증식에 미치는 식물생장조절물질의 영향
Influence of Plant Growth Regulators on Shoot Multiplication of Thymus quinquecostatus Celak.
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백리향(Thymus quinquecostatus Celak.)의 기내번식 방법을 개발하기 위해 줄기증식에 미치는 싸이토키닌 효과를 조사하 였다. 시료식물은 MS배지에 thidiazuron 등 5종의 싸이토키닌 을 단독 또는 혼합 처리하여 광배양 또는 암배양 조건에서 식물체 증식을 유도하였다. 백리향의 대량증식에 효과적인 cytokinin 종류와 농도는 명배양시 줄기수에 있어서 BA 1.0＋ TDZ 0.1 mg/L (6.50±1.51) 였고, 신초의 길이는 zeatin 2.0＋ TDZ 0.1 mg/L (3.27±0.7)가 최적 조건이었다. 또한 암배양시 줄기수의 경우엔 처리간에 별다른 차이 없이 비슷한 경향을 나타낸 가운데 2-ip 2.0 mg/L로 처리하였을 때가 가장 뛰어났 고, 줄기의 신장은 zeatin 2.0 mg/L일 때가 가장 효과적이었다. 전체적으로 기내에서 대량증식율은 평균 89.58%로 비교적 대부분의 시료에서 식물체가 유도되었다.
Abstract
In vitroculture system was established to induce multiple shoots of Thymus quinquecostatus Celak.by investigating the effects of cytokinins. Stem explants were cultured on MS medium supplemented with either fivedifferent plant growth regulators or their combinations under light or dark condition. The most effective cytokininsource was the combination of BA 1.0 mg/L and TDZ 0.1 mg/L for producing shoots (6.50± 1.51), zeatin 2.0 mg/Land TDZ 0.1 mg/L for elongating shoots (3.27 ± 0.66 cm) under the light condition. In addition, the most effectivecytokinin was 2-ip 2.0 mg/L for producing shoots (5.20 ± 1.81), zeatin 2.0 mg/L for elongating shoots (5.64± 1.24cm) under the dark condition. Overall, the average percent for in vitroshooting was greater than 89.58%.
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