할미 꽃 (Pulsatilla koreana NAKAI) 식물 절편체로부터 부정아 유도에 미치는 cytokinin의 영향 및 식물체 재 분화
Effect of cytokinin on adventitious shoot formation and plant regeneration from explants of Pulsatilla koreana NAKAI
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초록
본 실험에서는 할미 꽃의 엽병과 엽조직을 재료로 하여 부정아 형성에 미치는 cytokinin 영향을 조사하였다. 절편체는 0.5~3.0 mg/L 의 zeatin, BAP, Kinetin을 0.05 mg/L의 IAA와 조합 한 부정아 유도 배지에 치상하여 부정아를 유도시켰다. 배양 42일 후, 엽병을 MS 기본배지에 0.5 mg/L의 zeatin과 0.5 mg/L의 NAA를 첨가한 배지에 서 배양하였을 때 부정아 유도 효과가 가장 좋았으며, kinetin이 첨 가 된 배지에서 부정아 유도 효과도 양호하였다. 유도된 부정아 는 여러 덩어리로 잘라 MS배지에 0.5 mg/L BAP와 0.5 mg/L NAA를 첨가한 배지에서 증식 시켰으며 배양 7~10후, 식물체는 기내 발근 배지에 옮겨 졌다. 기내 발근은 1/2 MS배지에 1.5 mg/L NAA가 첨 가된 배지에서 가장 좋았으며 발근 된 식물체는 포트에 옮겨 순화 시켰다.
Abstract
Leaf and petiole explants of Pulsatilla koreana NAKAI were cultured on MS medium supplemented with various concentrations of zeatin, kinetin or BAP combined with 0.05 mg/L IAA. After 6 weeks of culture, effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole explants were cultured on medium with 0.5 mg/L zeatin and 0.05 mg/L IAA. Regenerated shoot were transferred on to root induction medium. The best root formation was observed at 1/2 MS medium with 1.5 mg/L NAA. Rooted plantlets were transplanted to a mixture of perlit and soil (1:3), where they were successfully acclimatized.
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