자생붓꽃의 형태적 특성 및 RAPD 마커에 의한 유연관계 분석
Morphological Characteristics and Genetic Relationship by RAPD Marker in lris spp.
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참고문헌
초록
본 연구는 붓꽃속 식물의 형태적 특성 조사와 RAPD 분석 을 통해 유전적 다양성과 근연성을 밝히고 유전자 도입을 위 한 기초적인 자료를 얻기 위하여 실시하였다. RAPD를 이용 하여 국내에서 수집한 자생붓꽃종의 DNA 다형성을 조사한 결과 40 개의 primer중 8개의 유용한 primer가 선발되었고 이 들 primer는 108 개의 밴드를 나타내었다. 이들 중 다형성 밴 드는 107 개였다. Jaccard (1-SJ)법에 의한 비유사도 계수는 0.095~0.699로 나타났고, dendrogram에서 나타난 유전적 근연 성은 크게 세 개의 군으로 분류되었다. I 군은 연미붓꽃, II 군 은 타래붓꽃, 제비붓꽃, 노랑꽃창포, 노랑무늬붓꽃이 속해 있었 으며, III 군은 금붓꽃, 각시붓꽃, 붓꽃, 꽃창포가 속해 있었다.
Abstract
This study was conducted to observe the morphological characteristics and to analyse geneticrelationship using RAPD markers in 9 species of Iris. The period of flowering was shown very broad among 9species of Iris, from April 22 to June 9. The flower stalk was fluctuated from 9.0 cm (I. rossii) to 104.0 cm(I.ensata). I. ensatahas the largest flower size and followed by I. laevigata, I. tectorum, I. sanguinea , I. pseudacorus, I.lactea, I. rossii, I. minutoaurea, I. odaesanensis. Among the 108 amplified bands by PCR with 8 random primers,107 showed polymorphism and only one showed monomorphism among 9 species of Iris. The averagedissimilarity coefficient among 9 species of Iriswas 0.252. The range of dissimilarity coefficient was shown as from0.095 to 0.609. Nine species were divied into three groups. The first group was I. tectorum, and the second groupwas I. lactea, I. laevigata, I. pseudacorus, I. odaesanensis. I. minutoaurea, I. rossii, I. sanguinea , I. ensatawere belongto group three.
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