Micropropagation of Hypericum erectum Thunbergby using Thidiazuron
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The effect of plant growth regulators was investigated on in vitro shoot proliferation from axillary bud explantsof Hypericum erectum. To determine the optimal cytokinin for proliferation of axillay buds, we carried out screening four cytokinins(BA, kinetin, 2iP, TDZ). When nodal segments were cultured on MS medium supplemented with 4.5 μM TDZ (thidiazuron),a number of shoots were induced. Our results indicated that the addition of TDZ to culture medium resulted in theinduction of significantly more axillary buds than in the addition of other cytokinins. The optimal concentration of TDZ forproliferation of axillary buds was 10 μM. 92% of shoots spontaneously rooted without any plant growth regulator (PGR) andformed whole plantlets within one month. More than 95% of these regenerants survived and they did not show any detectablevariation in morphology or growth characteristics compared to their donor plants.
Abstract
The effect of plant growth regulators was investigated on in vitro shoot proliferation from axillary bud explantsof Hypericum erectum. To determine the optimal cytokinin for proliferation of axillay buds, we carried out screening four cytokinins(BA, kinetin, 2iP, TDZ). When nodal segments were cultured on MS medium supplemented with 4.5 μM TDZ (thidiazuron),a number of shoots were induced. Our results indicated that the addition of TDZ to culture medium resulted in theinduction of significantly more axillary buds than in the addition of other cytokinins. The optimal concentration of TDZ forproliferation of axillary buds was 10 μM. 92% of shoots spontaneously rooted without any plant growth regulator (PGR) andformed whole plantlets within one month. More than 95% of these regenerants survived and they did not show any detectablevariation in morphology or growth characteristics compared to their donor plants.