삽주(Atractylodes ovata (Thunb.) DC.)의 효율적인 기내 줄기 재분화
An efficient in vitro shoot regeneration system for Atractylodes ovata (Thunb.) DC.
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초록
In this study, a plant tissue culture system was established for Atractylodes spp., an economically valuable medicinal crop in Korea that has low domestic production and is increasingly imported. In particular, Atractylodes ovata was treated with four types of cytokinins, 6-benzylaminopurine (BA), zeatin, kinetin, and thidiazuron (TDZ), in two different concentrations (0.5 and 1.0 mg/L). Among the four types of cytokinins, the BA treatment was effective for the shoot and root growth of A. ovata. Both the 0.5 mg/L and 1.0 mg/L concentrations of BA showed similar results; however, the 1.0 mg/L concentration of BA was more effective in promoting shoot and root growth. The treatments showed that the TDZ treatment was not effective for the shoot and root growth, except for the number of shoots and the fresh weight (FW) of the root; therefore, it was unsuitable for this species. In this study, we established a mass production system of A. ovata. Our results showed that direct in vitro regeneration may make a significant contribution to improving the cultivation of the medicinal plant A. ovata.
Abstract
In this study, a plant tissue culture system was established for Atractylodes spp., an economically valuable medicinal crop in Korea that has low domestic production and is increasingly imported. In particular, Atractylodes ovata was treated with four types of cytokinins, 6-benzylaminopurine (BA), zeatin, kinetin, and thidiazuron (TDZ), in two different concentrations (0.5 and 1.0 mg/L). Among the four types of cytokinins, the BA treatment was effective for the shoot and root growth of A. ovata. Both the 0.5 mg/L and 1.0 mg/L concentrations of BA showed similar results; however, the 1.0 mg/L concentration of BA was more effective in promoting shoot and root growth. The treatments showed that the TDZ treatment was not effective for the shoot and root growth, except for the number of shoots and the fresh weight (FW) of the root; therefore, it was unsuitable for this species. In this study, we established a mass production system of A. ovata. Our results showed that direct in vitro regeneration may make a significant contribution to improving the cultivation of the medicinal plant A. ovata.
참고문헌 (20)
- Animal and Plant Quarantine Agency, "2021 Year book of plant quarantine statics", pp.226~ (2021)
- Bizeng M, "Effects of plant growth regulators on the rapid proliferation of shoots and root induction in the Chinese traditional medicinal plant Atractylodes macrocephala", Frontiers of Biology in China, 4, pp.217~221 (2009)
- Demir S, "Effects of plant growth regulators on plant height and quantitative properties of Narcissus tazetta", Turk J Agric For, 43, pp.105~114 (2019)
- Fratini R, "Comparative study of different cytokinins in the induction of morphogenesis in Lentil(Lens Culinaris Medik)", In Vitro Cellular & Developmental Biology-Plant, 38, pp.46~51 (2002)
- Jun X, "Pharmacological effects of medicinal components of Atractylodes lancea (Thunb.) DC", Chinese Medicine, 13(1), pp.1~10 (2018)
- 고우리, "기내배양 백출 교잡종 ‘다출’(Dachul, Atractylodes macrocephala x A. japonica)에 미치는 생장조절제처리효과", 한국자원식물학회지, 24(5), pp.591~598 (2011), 한국자원식물학회
- Korea Forest Service(KFS), "2017 Production of Forest Products", pp.52~ (2018)
- Korea Forest Service(KFS), "2020 Production of Forest Products", pp.23~ (2021)
- 임현, "Effects of the Rhizomes of Atractylodes japonica and Atractylenolide I on Allergic Response and Experimental Atopic Dermatitis", Archives of Pharmacal Research, 35(11), pp.2007~2012 (2012), 대한약학회
- Miller WB, "Current status of growth regulator usage in flower bulb forcing in north america", Floric Ornament Biotechnol, 6, pp.35~44 (2012)
- National Institute of Horticultural and herbal science(NIHHS), "Guideline for GAP cultivation technology of medicinal crops", pp.128~141 (2016)
- Noboru H, "Cold storage of Atractylodes ovata shoot cultures and evaluation of the regenerated plants", Plant Biotechnology, 20(4), pp.347~351 (2003)
- Song G, "Establishment of large-scale tissue culture and rapid propagation system for Atractylodes lancea", Acta Agriculturae Jiangxi, 30(9), pp.63~67 (2018)
- Yukihiro S, "Clonal Multiplication of Atractylodes lancea by Tip Tissue Culture", Shoyakugaku Zasshi, 41(4), pp.313~317 (1987)
- Yuling L, "Direct shoot regeneration in vitro for Atractylodes macrocephala using epicotyl and radicleas explant", Journal of Hebei University (2020)[DOI]
- Yun PS, "Optimization of proliferation culture medium of Atractylodes lancea (Thunb.) DC. subsp. luotianensis S. L. Hu et X. F. Feng", Medicinal Plant, 1(11), pp.18~20 (2010)
- Zhu YQ, "Study on tissue culture and rapid propagation of Atractylodes macrocephala", Journal of Chinese medicinal materials, 29(3), pp.212~213 (2006)
- 윤보라, "백출의 Atractylenolide I과 Atractylenolide III의 함량분석", 생약학회지, 44(1), pp.53~59 (2013), 한국생약학회
- Zhang WJ, "Atractylodis Rhizoma : A review of its traditional uses, phytochemistry, pharmacology, toxicology and quality control", Journal of Ethnopharmacology, 266, pp.113415~ (2021)
- Zhu B, "The traditional uses, phytochemistry, and pharmacology of Atractylodes macrocephala Koidz.: a review", Journal of Ethnopharmacology, 226, pp.143~167 (2018)