산마는 다신초 덩어리로부터 인경 형성과 비대에 미치는 methyl jasmonate의 영향
Effect of Methyl Jasmonate on in vitro Bulblet Formation and Enlargement from Shoot Clump of Allium victorialis
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산마늘의 다신초 덩어리로부터 인경형성을 위해 ABA와 MeJA가 첨가된 MS 배지에서 배양하였다. 저농도 (0.01 mg/L)의 ABA 처리구는 인경 형성보다 오히려 신초의 증식을 유도 하였다. 인경은 MeJA 처리구에서 배양 4~6주 후 형성되기 시작하여 1.0 mg/L MeJA 처리구에서 다신초 덩어리로부터 100% 인경형성이 이루어졌다. 조직학적 관찰 결과 생장조절 제가 첨가되지 않은 배지의 인경과는 달리 MeJA 처리구에서 비대된 인경의 외피세포는 크고 둥글며 단백질 등의 물질로 가득 차 있었다. 산마늘의 다신초 덩어리로부터 인경형성에 MeJA가 촉진적임을 알 수있었다.
Abstract
The effect of abscisic acid (ABA) and methyl jasmonate (MeJA) on bulblet formation from the cultureof Allium victorialis var. platyphyllum Makino was studied. Shoot clumps were cultured on MS medium containingABA (0, 0.01~ 2.0 mg/L) and MeJA (0.01 ~ 5.0 mg/L). ABA at low concentrations (0.01 mg/L) induced shootproliferation without bulblet formation. However, bulblet formation started on the medium containing MeJAapproximately in 4-6 weeks of culture. Furthermore, 1.0 mg/L MeJA resulted in bulblet formation at high frequency(100%) as compare to the control (46.1%). Cortical cells of the bulblets enlarged on medium with MeJA had denseprotein-like substance in expanded and round cells when examined under the microscope. The data described hereshow that formation and enlargement of bulblets from Allium victorialis can be improved by addition of appropriateconcentration of MeJA.
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