In vitro Culture and Shoot Proliferation of Wild Garlic(Allium ochotense Prokh.)
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초록
An efficient method for in vitro propagation and growth of the wild garlic(Allium ochotenseProkh.) was established. Bulbs of wild garlic were collected from Ullung Island, Korea, and thegrowth pattern of plantlets on various culture media was observed. High growth of shoot wasobtained on LP, NN and N6 medium. The growth media supplemented with 3%(w/v) sucrosewas found optimal for shoot growth. After 10 weeks multiple shoots were observed in theplantlets growing on the medium containing 1.0 mg/l of zeatine and 0.1 mg/l NAA. Roots wereinduced directly at the base of the shoot in all treatments. The medium with 2.0 mg/l of IBAproved to be the best rooting medium. The studies of this kind may be used to developstrategies for large-scale propagation of elite wild garlic.
Abstract
An efficient method for in vitro propagation and growth of the wild garlic(Allium ochotenseProkh.) was established. Bulbs of wild garlic were collected from Ullung Island, Korea, and thegrowth pattern of plantlets on various culture media was observed. High growth of shoot wasobtained on LP, NN and N6 medium. The growth media supplemented with 3%(w/v) sucrosewas found optimal for shoot growth. After 10 weeks multiple shoots were observed in theplantlets growing on the medium containing 1.0 mg/l of zeatine and 0.1 mg/l NAA. Roots wereinduced directly at the base of the shoot in all treatments. The medium with 2.0 mg/l of IBAproved to be the best rooting medium. The studies of this kind may be used to developstrategies for large-scale propagation of elite wild garlic.
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