Rapid micropropagation of wild garlic (Allium victorialis var. platyphyllum) by the scooping method
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.
Abstract
Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.
참고문헌 (24)
- Alejandro FAB, "Development of in vitro techniques as supportive tools for breeding and mass clonal propagation of Leucocoryne spp. (Amaryllidaceae)", Doctor, pp.2016
- Ayabe M, "Establishment of a novel tissue culture method, stem-disc culture, and its practical application to micropropagation of garlic (Allium sativum L.)", Plant Cell Rep, 17, pp.773~779 (1998)
- Choi SJ, "Plant regeneration from Allium victorialis plant parts", J Agr Sci, 17, pp.17~22 (2006)
- De Klerk GJ, "Micropropagation of bulbous crops: Technology and present state", Floric Ornam Biotechnol, 6, pp.1~8 (2012)
- 홍문표, "강원도 설악산의 식물상", 한국환경생태학회지, 24(4), pp.436~486 (2010), 한국환경생태학회
- 정미진, "Influence of Nitrogen, Phosphorous, Potassium and Sucrose on the Shoot and Bulb Growth of Allium victorialis var. platyphyllum", 한국약용작물학회지, 20(2), pp.101~107 (2012), 한국약용작물학회[DOI]
- Kamstaityte D, "Micropropagation of onion (Allium cepa L.)", Acta Univ Latviensis Biol, 676, pp.173~176 (2004)
- Kim MJ, "Wild garlic cultivation technology" (2014), National Institute of Forest Science
- Kumar S, "Effects of a garlic active based growth promoter on growth performance and specific pathogenic intestinal microbial counts of broiler chicks", Int J Poult Sci, 9, pp.244~246 (2010)
- 권영희, "코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을통한 다신초유도와 종구대량증식 시스템", 한국자원식물학회지, 31(4), pp.355~362 (2018), 한국자원식물학회[DOI]
- Cheul Ho Lee, "In vitro Culture and Shoot Proliferation of Wild Garlic(Allium ochotense Prokh.)", 농업생명과학연구, 52(2), pp.73~80 (2018), 농업생명과학연구원[DOI]
- Lu CY, "The use of thidiazuron in tissue culture", In Vitro Cell Dev Biol Plant, 29, pp.92~96 (1993)
- Maesato K, "In vitro bulblet regeneration from bulb scale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment", J Hortic Sci, 69, pp.289~297 (1994)
- Memon N, "In vitro propagation of gladiolus plantlets and cormels", J Hortic Sci Ornam Plants, 4, pp.280~291 (2012)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Murthy BNS, "Thidiazuron: a potent regulator of in vitro plant morphogenesis", In Vitro Cell Dev Biol Plant, 34, pp.267~275 (1998)
- Nam SI, "Commercial production of seed garlic by tissue culture technique", J Plant Biotechnol, 29, pp.171~177 (2002)
- Ohnuki-Tierney E, "Illness and Healing Among the Sakhalin Ainu: A Symbolic Interpretation (preview)", pp.192~ (1981), Cambridge University Press
- Park YJ, "Effective and economical propagation method of Lycoris squamigera native to Korea", Hortic Sci Technol, 16, pp.242~243 (1998)
- Paulos MM, "Effect of IAA and IBA on In vitro Rooting of Banana (Musa paradisiaca) Cv.Grand Naine", Int J Sci and Res, 4, pp.959~962 (2015)
- Quoirin M, "Improved media for in vitro culture of Prunus sp", Acta Hortic, 78, pp.437~442 (1977)
- Saniewski M, "Effect of ethrel and morphactin on bulblet differentiation in scooped out hyacinth (Hyacinthus orientalis L.) bulbs", Bull Acad Pol Sci Biol, 25, pp.107~110 (1977)
- Xu Z, "Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation", Acta Physiol Plant, 30, pp.521~528 (2008)
- Zheng SJ, "The development of an efficient cultivar-independent plant regeneration system from callus derived from both apical and non-apical root segments of garlic (Allium sativum L.)", In Vitro Cell Dev Biol Plant, 39, pp.288~292 (2003)