코끼리마늘(Allium ampeloprasum L.)의 기내 미숙총포 배양을통한 다신초유도와 종구대량증식 시스템
Multiple Shoot Induction and Bulb Mass Proliferation System by in Vitro Immature Spathe Culture of Elephant Garlic (Allium ampeloprasum L.)
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초록
본 연구는 증식이 어려운 코끼리마늘(Allium ampeloprasum L.)의 종구 보급을 위해 기내배양 기술을 이용한 대량증식 시스템을 개발하기 위해 수행되었다. 코끼리마늘의 미숙총포를 수확 직후 길이별로 채취하여 MS배지에 NAA와 kinetin 생장조절제를 혼합 농도 처리하여 배양한 결과, 10 ㎝ 길이로 채취하였을 때가 절편체당 신초수 14.9개로 가장 많이 얻어졌다. 생장조절제 농도에 따른 생장을 조사한 결과, kinetin 2 ㎎/L와NAA 0.5 ㎎/L를 첨가한 MS배지에서 절편체당 신초수가 14.9 개로 가장 많았고 초장은 11.3 ㎝였으며, 생체중은 2.5 g으로가장 좋았다. 코끼리마늘 기내소구 형성에 적합한 최적 배지조성은 생장조절제 kinetin 2 ㎎/L, NAA 0.5 ㎎/L를 첨가한 MS 배지에 다신초 유기물질인 adenine 30 ㎎/L를 첨가하였을 때구 형성률이 48%로 높았으며, 이 배지에 sucrose 7%를 첨가하였을 때 기내종구 형성률이 98.2%로 증가하였다. 다신초 유도를 위한 계대배양은 1차 배양 단계보다 2차 계대배양 단계에서신초 증식배율이 좋았으며, 2차 계대배양에서 구 형성률은80%로 높게 나타났다.
Abstract
This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture. Key words - Bulb, Elephant garlic (Allium ampeloprasum L.), Immature spathe, in vitro, Propagation
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