돌나물 엽절편으로부터 캘러스 형성 및 식물체 분화에 미치는 생장조절물질의 영향
Effects of Growth Regulators on Callus Induction and Plant Regeneration from Leaf Explants of Sedum sarmentosum
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초록
돌나물 (Sedum samentosum)의 기내 식물체 재분화 체계를 확립하기 위하여, 생장조절물질을 다르게 조합한 MS 배지에 잎절편을 치상한 후, 캘러스 유기 및 식물체 분화에 미치는 생장조절물질의 영향을 조사하였다. 캘러스 유기는 3.0 mg/L 2, 4-D와 1.0 mg/L BA 첨가 배지에서 가장 높은 캘러스 유기 율을 보였으나, NAA와 BA 혼합첨가 배지에서는 캘러스 형성 율이 0~3% 이하로 극히 낮았다. 식물체 분화는 NAA 단독첨 가 배지에서는 전혀 분화되지 않았으며, 0.2 mg/L NAA와 3.0 mg/L BA 첨가배지에서 가장 양호하였다. 캘러스를 식물체 분 화배지에 계대배양 후 50일까지는 신초수 증가와 함께 (17.2 개/캘러스), 식물체의 기형이나 잎비대 현상도 적어 식물체 분 화배지로 가장 적합하였다. 분화 식물체는 생장조절물질을 첨 가하지 않은 MS 기본배지로 계대배양하였을 때 발근 및 생육 이 양호하였다.
Abstract
To establish the system of in vitro plant regeneration, the leaf segments of Sedum sarmentosum were cultured on MS media supplemented with different levels of 2,4-D, NAA and BA. The callus induction and growth showed a good response on MS medium supplemented with 3.0 mg/L 2,4-D and 1.0 mg/L BA, but a few callus induced on medium containing NAA and BA. In plant regeneration, combination of BA and NAA promoted shoot organogenesis from callus, and the highest frequency was obtained on MS medium supplemented with 0.2 mg/L NAA and 3.0 mg/L BA. When calli were transferred to the plant regeneration medium containing 0.2 mg/L NAA and 3.0 mg/L BA, healthy shoots without hyperhydricity were continuously induced (17.2 plantlets per callus) after 50 days of culture. When regenerated plantlets were transferred onto hormone-free MS medium, rooting was easily achieved from all of them.??????
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