국화 ‘백마’의 잎 절편체로부터 신초 기관형성을 통한 식물체 재생에 적합한 배지조성 및 배양방법
The Optimal Medium Composition and Culture Method for Plant Regeneration via Adventitious Shoot Formation from Leaf Segment Explants of Chrysanthemum morifolium ‘Baekma’
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초록
Plant regeneration protocols via adventitious shoot organogenesis from leaf segments of Chrysanthemum morifolium‘Baekma’ were developed. The effects of plant growth regulators (BA, NAA, IBA, IAA and 2,4-D) and AgNO3 were tested tofigure out the optimal condition for shoot bud induction and shoot formation from leaf explants. On the combination treatmentof plant growth regulators and AgNO3, bud induction was obtained but shoot formation was not. Therefore, two-stage treatmentof leaf explants was subsequently experimented for the respective improvement of adventitious bud induction and shootformation. When leaf explants were cultured on bud induction medium (MS medium supplemented with 0.2 mg/L BA, 1.0 mg/LIAA and 1.0 mg/L 2,4-D) for 4 weeks in darkness and were transferred to shoot formation medium (MS medium supplementedwith 1.0 mg/L BA, 1.5 mg/L IBA and 5.0 mg/L AgNO3) for 4 weeks under the 16/8h photoperiod condition, shoot formationefficiency reached up to 50.0% and 1.6 shoots per explant were acquired. At 12 weeks after culture, the regenerated shoots wereelongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants exhibited normalphenotypes.
Abstract
Plant regeneration protocols via adventitious shoot organogenesis from leaf segments of Chrysanthemum morifolium‘Baekma’ were developed. The effects of plant growth regulators (BA, NAA, IBA, IAA and 2,4-D) and AgNO3 were tested tofigure out the optimal condition for shoot bud induction and shoot formation from leaf explants. On the combination treatmentof plant growth regulators and AgNO3, bud induction was obtained but shoot formation was not. Therefore, two-stage treatmentof leaf explants was subsequently experimented for the respective improvement of adventitious bud induction and shootformation. When leaf explants were cultured on bud induction medium (MS medium supplemented with 0.2 mg/L BA, 1.0 mg/LIAA and 1.0 mg/L 2,4-D) for 4 weeks in darkness and were transferred to shoot formation medium (MS medium supplementedwith 1.0 mg/L BA, 1.5 mg/L IBA and 5.0 mg/L AgNO3) for 4 weeks under the 16/8h photoperiod condition, shoot formationefficiency reached up to 50.0% and 1.6 shoots per explant were acquired. At 12 weeks after culture, the regenerated shoots wereelongated and rooted on MS medium. The plantlets were acclimatized successfully and the regenerated plants exhibited normalphenotypes.
참고문헌 (25)
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