둥근잎꿩의비름(Sedum rotundifolium D. Lee)의 식물체 재분화에 미치는 식물생장조절제의 영향
Effect of plant growth regulators on plant regeneration from the Sedum rotundifolium D. Lee
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둥근잎꿩의비름 (Sedum rotundifolium D. Lee)의 기내 식물체 재분화 체계를 확립하기 위하여, 생장조절물질을다르게 조합한 MS 배지에 화뢰와 잎 절편체를 치상하여캘러스 유도와 식물체 재분화에 미치는 영향을 조사하였다. 캘러스 유기는 화뢰절편체에서 보다 잎절편체에서왕성하게 관찰되었으며, 1.0 ~ 2.0 mg/L NAA와 1.0 mg/L BA의 혼합첨가 배지에서는 캘러스의 형성률이 100%로가장 높았으며, 캘러스의 증식도 매우 왕성하였다. 또한,2.0 mg/L 2,4-D에 1.0 mg/L BA를 혼합처리 한 배지에서유도된 캘러스를 치상하여 8주간 배양하였을 때 가장많은 신초가 유도되었고, IAA를 단독처리 한 MS 배지에서 보다 더 정상적이며 성장이 양호한 뿌리의 분화가관찰되었다. 재분화 식물체는 식물생장조절물질이 첨가되지 않은 MS배지로 계대배양하여 생장시킨 후 인공토양의 화분으로 옮겨 관찰한 결과 100%의 생존률을 보이며 정상적으로 생육하여 재분화 식물체를 대량으로 획득하였다.
Abstract
To establish the system of In vitro plant regeneration,the floral bud and leaf explants of Sedum rotundifolium were cultured on the MS media supplemented with different concentration of 2,4-D, NAA, and BA. The callus induction was more effective in the floral explants than the leaf explants, and was the best on MS medium containing 1.0 or 2.0 mg/L 2,4-D and 1.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L BA for 8weeks. The normal root formation from shoot was effective on the MS medium containing IAA alone. The regenerated plantlets were transferred to the pot and acclimatized successfully.
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