멸종위기식물 피뿌리풀의 기내증식
Micropropagation of Endangered Species, Stellera rosea Nakai by Tissue Culture
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초록
멸종위기에 직면한 피뿌리풀 (Stellera rosea N.)의 기내증식법 을 개발하고자 액아 마디를 MS 배지에 BAP와 zeatin을 처리하여 다경 (multiple shoot)를 유도하고 기내발근에 미치는 IBA 및 NAA 처리 효과를 조사하였다. 액아 마디로부터 다경 유도는 BA가 현저히 양호한 반면 줄기의 생장은 zeatin이 BA보다 효과적이었다. 증식된 줄기로부터 기내 발근은 오옥 신 처리로 가능하였으나 발근율은 대체로 저조하였고 오옥신 의 전처리 기간에 따라 차이를 보였다. IBA가 NAA보다 다소 좋은 발근효과를 보였고 1.0 mg/L 농도로 15일간 배양시 30% 까지 발근되었다. NAA 역시 처리농도 및 처리기간에 따라 발 근율에 차이를 보였다. 발근묘는 인공토양에서 51%가 활착되 어 정상생장이 가능하였다. 이상의 결과는 발근 및 순화조건 을 좀더 개선하면 피뿌리풀의 효율적인 기내번식이 가능함을 시사해준다.
Abstract
In order to develop an efficient micropropagation technique for an endangered species, Stellera roseaN., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was betterthan zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. Invitrorooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate wasrelatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0 mg/L IBArevealed a slightly better rooting efficiency reaching 30% rooting rate than NAA. Root induction rate by NAA alsovaried with concentration of NAA and culture periods. Total 51% of the rooted plantlets survived on artificial soilmixture and grew normally without any distinct morphological variation. The results suggest that the endangeredStelleraplants are propagated via in vitro culture system, but still need to more study for the improvement of rootingand acclimatization of the plantlets in soil.
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