배지첨가물질이 이탈리안 라이그래스의 종자유래 캘러스배양에 미치는 영향
Effects of Medium Supplements on Seed-derived Callus Culture of Italian ryegrass
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이탈리안 라이그래스의 최적 조직배양조건을 확립하기 위하여 'Jeanne', 'Florida-80', 'Metro' 세 가지 품종의 성숙종자로부터 배발생 캘러스 유도 및 캘러스로부터 식물체 재분화에 미치는 배지 첨가물질의 영향을 조사하였다. 성숙종자로부터 배발생 캘러스 유도시에 첨가되는 식물생장조절물질로는 모든 품종에서 2,4-D의 경우 5mg/L의 농도로 첨가된 MS 배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었으며, dicamba의 경우 7mg/L의 농도로 첨가해주었을 때 배발생 캘러스의 유도율이 가장 높았다. 배발생 캘러스로부터 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 5mg/L BA가 첨가된 N6 배지에서 배양했을 때 가장 높은 식물체 재분화율을 나타내었다. 광조건에 따른 배양효율의 차이는 모든 품종에서 배발생 캘러스의 유도시에 암상태에서 배양한 캘러스로부터 식물체 재분화율이 광상태에서 배양한 캘러스의 재분화율 보다 약 10%정도 높게 나타났다. Casein hydrolysate와 L- proline의 경우 동시에 배지에 혼용 첨가해 주었을 때 배발생 캘러스 유도율과 식물체 재분화율이 증가되었다. 본 연구를 통하여 확립된 효율적인 배발생 캘러스의 유도 및 식물체 재분화체계는 분자육종을 통한 신품종 이탈리안 라이그래스의 개발에 유용하게 이용되어질 수 있을 것이다.
Abstract
In an effort to optimize tissue culture responses of Italian ryegrass(Lolium multiflorum Lam.) for future genetic manipulations to improve forage characteristics, the effects of culture medium supplements on tissue culture responses were investigated with mature seeds of three cultivars, 'Jeanne', 'Florida-80' and 'Metro', as explant tissues. For all explants, MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The optimal concentration of dicamba for the induction of embryogenic callus from mature seeds was 7mg/L. The highest plant regeneration frequency was observed when embryogenic callus was transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Plant regeneration frequency of callus cultured in the dark was higher than that of cultured in the light. Casein hydrolysate and L-proline improved both in embryogenic callus induction from mature seeds and plant regeneration. High-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.
참고문헌 (23)
- "An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue(Festuca arundinacea Schreb.) cultivars", 55, pp.326~330 (2000)
- "Somatic embryogenesis and plant regeneration of turf-type bermudagrass effect of 6-BA in callus induction medium", 60, pp.113~120 (2000)
- "Transformation of recalicitrant barly cultivars through improvement of regenerability and decreased albinism", 138, pp.229~244 (1998)
- "A cold-tolerant and high-yielding Italian ryegrass(Lolium multiflorum Lam.) new variety 'Hwasan 101'", 20, pp.1~6 (2000)
- "Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources", pp.659~668 (1975)
- "The effect of 2 4-dichlophenoxyacetic acid and donor plant environment on plant regeneration from immature inflorescence derived callus of Lolium perenne L", pp.165~172 (1988), Creemers-Molenaar
- "Embryogenesis from cultured immature inflorescences and nodes of Lolium multiflorum", pp.47~55 (1981)
- "Co-transformed, diploid Lolium perenne Lolium multiflorum and Lolium temulentum plants produced by microprojectile bombardment", 18, pp.721~726 (1999)
- "Forage and turf grass biotechnology: principles, methods and prospects. In: Setlow, J. K.(Ed.),", 21, pp.`191~ (1999), Kluwer Academic Publishers
- "High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrassPoa pratensis L.).", 14, pp.721~724 (1995)
- "Seed development and seed yield potential of Italian ryegrass", pp.181~188 (1993)
- "Influence of slight shading sward density and nitrogen fertilization on yield and nutritive value of Lolium multiflorum Lam", pp.341~347 (1993)
- "Plant regeneration from isolated ovules of Italian ryegrass effect of 2 4-dichlorophenoxyacetic acid and different cytokinins supplemented to the ovule culture medium", 111, pp.107~116 (1995)
- "Factors affecting plant regeneration from seed-derived calli in Italian ryegrassLolium multiflorum Lam", 28, pp.323~328 (2001)
- "Improving forage production systems using biotechnolgy. In: McKersie, B. D. and Brown, D. C. W.(Eds),", 17, pp.3~ (1997), Wallingford
- "A revise medium for rapid growth and bioassays with tobacco tissue cultures", pp.473~497 (1962)
- "A comparison between diploid and tetraploid cultivars of Lolium multiflorum Lam", pp.135~139 (1987)
- "Callus induction from seeds of Italian ryegrass and plant regeneration", 20, pp.25~30 (2000)
- "Biotechnology in forage and turf grass improvementIn: Frankel et al(Eds),", 23, pp.192~ (1998), Springer Verlag
- "Achievements and prospectives in the breeding of forage grasses and legumes Grasslands for our world" (1993)
- "Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells", 16, pp.379~384 (1997)
- "Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes", 76, pp.277~281 (2004)
- "Plant regeneration via somatic embryogenesis in creeping bentgrass", pp.453~456 (1991)