Peace 포플러(Populus koreana X P.trichocarpa)의 줄기형성에 미치는 Thidiazuron 효과
Effect of TDZ (Thidiazuron) on Shoot Proliferation of Peace Poplar
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기공이 잎의 앞뒤 양면에 존재하는 생리·형태적 특징을 가지고 있는 외래 교잡종인 Peace (Populus koreana×P. trichocarpa) 포플러의 효율적인 기내증식법을 개발하고자 액아와 엽육 절편을 이용하여 기내배양을 실시하였다. 몇 종류의 배 지 시험을 통해 1/2 MS 배지를 적정배지로 선정할 수 있었고, 이 배지에 TDZ를 처리하여 multiple shoot 및 adventitious shoot 유도 효과를 조사하였다. 절간액아 및 엽육을 절편으로 TDZ의 농도별 처리로 배양 2주 후부터 multiple shoot 및 adventitious shoot가 유도되었으며 절편 및 TDZ 처리 농도에 따라 차이를 나타냈다. 액아로부터는 TDZ 처리로 절편당 5-7 개의 multiple shoot가 유도된 반면 엽육 절편에서는 2-5개의 adventitious shoot가 유도되었다. 한편 부정아로부터 정상적인 줄기의 성장에 있어서도 절편에 따른 차이를 나타냈다. 이상 의 결과는 저농도의 TDZ의 처리로 peace 포플러의 액아 혹은 엽육 절편의 기내배양을 통해 대량증식이 가능함을 보여주는 결과이다.
Abstract
Shoot formation was investigated from in vitro cultivation of exotic hybrid poplar (Populus koreana × P. trichocarpa) with a specific stomatal character occurring both upper and lower surface of leaves. Two different explants (stem and leaf segment) of Peace poplar were cultured on half strength Murashige and Skoog (MS) basal medium supplemented with the various concentrations of thidiazuron as a plant growth regulator. Most adventitious shoots were produced from excised ends of stem or mid-veins of leaf segments. The highest average numbers of shoots were 7.1 and 5.3 with the treatments of 0.02 mg/L TDZ in both explants of stem and leaf segment. The highest shooting rates were achieved to 83.3% and 47.6% with the concentrations of 0.01 mg/L and 0.02 mg/L TDZ by axillary bud and leaf cultures, respectively.
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