Peace 포플러의 기내 배양시 발생하는 과수화 식물체의 조직적 특성
Anatomical Characteristics of Hyperhydric Shoots Occuring in In Vitro Culture of Peace Poplar
2
조회
0
다운로드
1
인용
0
참고문헌
초록
목본류 생리학 연구에 있어서 중요한 수종인 Peace 포플러의 기초 연구자료를 얻고자 본 실험을 실시 하였다. 액아 마디를 BA 0.2 mg/L 처리된 MS 배지에 배양하여 shoot의 증식시 나타나는 과수화 된 shoot를 정상 shoot와 비교하였다. 과수화 된 shoot는 진한 녹색을 띠고, 잎은 두껍고 휘어져 있으며 투명하였다. 또 줄기는 정상적인 줄기보다 두껍고 길이가 짧았다. 조직학적 관찰 결과 과수화 된 잎은 책상조직의 발달이 부진하였으며 정상적인 잎에 비하여 책상조직과 해면조직 그리고 표피 세포 모두 불규칙하고 세포간극의 크기가 큰 것으로 나타났다. 또한 과수화 된 잎의 기공은 크기가 커서 단위면적 당 밀도가 낮았으며 공변세포와 그 주변 세포들이 변형되어 있었다. 과수화된 shoot의 직경은 정상적인 것보다 큰 것으로 나타났는데 이것은 피층과 수층 세포의 비대에 따른 것으로 관찰되었다. 한편 과수화 된 줄기는 정상 줄기에 비해 목질화 발달이 미약하여 형성층 구별이 어려웠다.
Abstract
We investigated the anatomical aspects of vitrification in peace poplar. Comparisons were made with regard to characteristics occurring between hyperhydric and normal shoots in shoot proliferation cultures on MS medium containing 0.2 mg/L BA. Compared with normal plants, hyperhydric plants had thick, curled, and dark green leaves. Hyperhydric stems were thicker and shorter than those of normal stems. When examined under the microscopes, the mesophyll palisade cells of hyperhydric leaves were vacuolated, whereas those of normal leaves contained normal and enriched vacuole with cytoplasm. Generally, the hyperhydric leaves showed poorly developed palisade parenchyma, and revealed irregular and bigger sized intercellular structures in both palisade and spongy parenchyma as well as epidermis cells compare to those of normal leaves. In addition, the hyperhydric leaves had lower stomatal density and bigger sized cell. Vascular tissues of hyperhydic stems were less differentiated because of poorly lignified xylem tissue. The greatly expanded cortical cells and pith appeared to be the main cause of thick stems as compared with normal stems.
참고문헌 (26)
- Apostolo NM,, "Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinensis (Link) Schn", 36, pp.243~249 (2000)
- Chen J, "The effect of ancymidol on hyperhydricity starch and antioxidant enzymatic activities in liquid-cultured Narcissus", 20, pp.22~27 (2001)
- Cohen D, "Reconsideration of the term vitrification as used in micropropagation", pp.135~140 (1992)
- Edgard ATP,, "Hyperhydric in in vitro eggplant regenerated plants: structural characteristics and involvement of BiP (Binding Protein).", 160, pp.857~868 (2001)
- Fontes MA,, "Hyperhydricity in peper plants regeneration in vitro: Involvement of BiP (Binding Protein) and ultrastructural aspects", 19, pp.81~87 (1999)
- Furukawa A, "Hybrid poplar stomata unresponsive to changes in environmental conditions", 4, pp.191~197 (1990)
- Gribble K, "The influence of relative humidity on vitrification growth and morphology of Gypsophila paniculata L", 27, pp.179~188 (1999)
- Kadota M, "Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear", 89, pp.207~215 (2001)
- Kang HD,, "Effect of TDZ on shoot proliferation of Peace poplar.", 31, pp.49~53 (2004)
- Majada JP, "Impact of culture vessel ventilation on the anatomy morphology of micropropagation carnation", 63, pp.207~214 (2000)
- Marga F, "Agar fractions could protect apple shoots cultured in liquid media against hyperhydrity", 49, pp.1~5 (1997)
- Kadota M, "Effects of cytokinin types and their concentration on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots", 72, pp.261~265 (2003)
- Mayor ML, "Reduction of hyperhydricity in sunflower tissue culture", 72, pp.99~103 (2003)
- Miguens FC, "A scanning electron microscope study of normal and vitrified leaves from Datura insignis plantlets cultured in vitro", 32, pp.109~113 (1993)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", 15, pp.473~497 (1962)
- Olmos E, "Ultrastructural difference of hyperhydric and normal leaves from regenerated carnation plants", 75, pp.91~101 (1998)
- Olmos E, "The subcellular localization of peroxidase and the implication of oxidative stress in hyperhydrated leaves of regenerated carnation plants", 130, pp.97~105 (1997)
- Paek KY, "biochemical and morphological characteristics of vitrified shoots regenerated in vitro", pp.151~162 (1991)
- Park SY, "Effect of CO2 enrichment on growth of two poplar clones, I-214 (Populus euramericana) and Peace (P. koreana X P. trichocarpa).", 18, pp.255~263 (1995)
- Piqueras A, "Polyamines and hyperhydricity in micropropagated carnation plants", 162, pp.671~678 (2002)
- Park SW, "Effect of sealed and vented gaseous micro environments on the hyperhydricity of potato shoots in vitro", 99, pp.199~205 (2004)
- Ridolfi M,, "Responses to water stress in an ABA-unresponsive hybrid poplar (Populus koreana X trichocarpa cv. Peace", 135, pp.31~40 (1997)
- Thomas P,, "Explant, medium and vessel aeration affect the incidence hyperhydricity and recovery of normal plantlets in triploid watermelon", 75, pp.19~25 (2000)
- Ueno K,, "Reduced hyperhydricity and enhanced growth of tissue culture-generated raspberry (Rubus sp.) clonal lines by Pseudomonas sp. isolated from oregano", 33, pp.441~445 (1998)
- Whitehouse AB, "Control of hyperhydricity in Eucalyptus axillary shoot cultures grown in liquid medium", 71, pp.245~252 (2002)
- Yeung EC, "The use of histology in the study of plant tissue culture systems - some practical comments", 35, pp.137~143 (1999)