Shoot Regeneration from Leaf and Shoot derived Callus of Hybrid Poplar(Populus alba x P. glandulosa) Clone
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.
Abstract
Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.
참고문헌 (25)
- Ahuja MR, "Somatic cell differentiation and rapid clonal propagation of aspen", Silvae Genet, 32, pp.131~135 (1983)
- Ahuja MR, "Cell and tissue culture in forestry", pp.207~223 (1987), Springer
- Chun YW, "Biotechnological applications in Populus species", J. Kor. For. Soc, 77, pp.467~483 (1988)
- Chun YW, "Biotechnology application of Populus micro propagation", pp.1987
- Coleman GD, "Axillary shoot proliferation and growth of Populus deltoides shoot cultures", Plant Cell Rep, 9, pp.165~167 (1990)
- Donaldson JR, "Age-related shifts in leaf chemistry of clonal aspen(Populus tremuloides)", J. Chem. Ecol, 32, pp.1415~1429 (2006)
- Greenaway W, "Relationships of Populus × acuminata and Populus x generosa with their parental species examined by gas chromatography-mass spectrometry of bud exudates", Can. J. Bot, 70, pp.212~221 (1992)
- Jafari MA, "High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes", Acta Biol. Hung, 46, pp.51~59 (1995)
- Plant regeneration through organogenesis in poplar. In micropropagation, genetic engineering and molecular biology of populus, "Kang H", USDA, 326~336, pp.USDA Forest Service Gen. Tech. Rep, RM-GTR-297 (1997)
- Kang H, "Morphological in vitro shoot abnormalities in hybrid aspen(Populus alba x P. grandidentata Michx.)", Korean J. Plant Tissue Culture, 23, pp.141~145 (1996)
- 김현철, "저농도 액비 처리에 의한 포플러 단벌기 맹아림의 바이오매스 생산", 한국육종학회지, 46(1), pp.10~16 (2014), 한국육종학회[DOI]
- Mellway RD, "The wound-, pathogen-, and ultraviolet B-responsive MYB134 gene encodes an R2R3 MYB transcription factor that regulates proanthocyanidin synthesis in poplar", Plant Physiol, 150, pp.924~941 (2009)
- Miranda M, "The transcriptional response of hybrid poplar(Populus trichocarpa x P. deltoids)to infection by Melampsora medusae leaf rust involves induction of flavonoid pathway genes leading to the accumulation of proanthocyanidins", Mol Plant Microbe Interact, 20, pp.816~831 (2007)
- Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue cultures", Physiologia Plantarum, 15, pp.473~497 (1962)
- Noh EW, "High efficiency shoot regeneration from callus of quaking aspen(Populus tremuloides Michx.)", Plant Cell Rep, 5, pp.464~467 (1986)
- Osier TL, "Long-term effects of defoliation on quaking aspen in relation to genotype and nutrient availability: Plant growth, phytochemistry and insect performance", Oecologia, 139, pp.55~65 (2004)
- Park YG, "In vitro organogenesis and somatic embryogenesis from punctured leaf of Populus nigra x P. maximowiczii", Plant Cell Tiss. Organ Cult, 15, pp.95~105 (1988)
- Rowland LJ, "Use of a cytokinin conjugate for efficient shoot regeneration from leaf sections of highbush blueberry", HortScience, 27, pp.1127~1129 (1992)
- Shahzad A, "Micropropagation through excised root culture of Clitoria ternatea and comparison between in vitro-regenerated plants and seedlings", Annals of Applied Biology, 150, pp.341~349 (2007)
- Son SH, "Plant regeneration by anther culture of tetraploid Populus alba L. x P. glandulosa Uyeki", Korean J. Plant Tissue Culture, 22, pp.121~125 (1995)
- Sugimoto, K, "Regeneration in arabidopsis tissue culture", Methods Mol. Biol, 959, pp.265~275 (2013)
- Sul IW, "Regeneation of adventitious shoots from Populus deltoides barter", J. Plant Biotechnol, 24, pp.329~334 (1997)
- Tsai CJ, "Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus", New Phytol, 172, pp.47~62 (2006)
- Winton LL, "Plantlets from aspen tissue cultures", Science, 160, pp.1234~1235 (1968)
- Wolter K, "Root and shoot initiation in aspen callus cultures", Nature, 219, pp.509~510 (1968)