대두 체세포배에서 전형성층 분화와 경단분열조직의 발달
Procambium differentiation and shoot apical meristem development in somatic embryos of soybean (Glycine max L.)
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Immature embryos of Glycine max L. was cultured on Murashige and Skoog’s (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). After 6 to 8 weeks of culture, immature embryos produced somatic embryos. Of somatic embryos, two cotyledonary embryo (14%), one cotyledonary embryo (37%), fused cotyledonary embryo (43%), and stunted globular embryos (6%) were observed. The procambial strand of cotyledons originated from circular procambial tissues of lower hypocotyl. The circular procambial tissues were independently divided into one or two procambial strand at the edge of cotyledonarynode,and then connected to each cotyledon to form somatic embryos with one or two cotyledons. When cotyledon was a fused type, the circular procambial strand in lower hypocotyl was continuously connected to the cotyledon. Also, somatic embryos with two cotyledons developed a functional shoot apex with the tunica-corpus structure. In contrast, somatic embryos with one or fused cotyledon formed an abnormal shoot apex without the tunica-corpus structure or with non-dome shape in the inter-cotyledonary area. These results indicated that the variation of cotyledon in somatic embryos is closely related to procambial differentiation and shoot apical meristem development.
Abstract
Immature embryos of Glycine max L. was cultured on Murashige and Skoog’s (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). After 6 to 8 weeks of culture, immature embryos produced somatic embryos. Of somatic embryos, two cotyledonary embryo (14%), one cotyledonary embryo (37%), fused cotyledonary embryo (43%), and stunted globular embryos (6%) were observed. The procambial strand of cotyledons originated from circular procambial tissues of lower hypocotyl. The circular procambial tissues were independently divided into one or two procambial strand at the edge of cotyledonarynode,and then connected to each cotyledon to form somatic embryos with one or two cotyledons. When cotyledon was a fused type, the circular procambial strand in lower hypocotyl was continuously connected to the cotyledon. Also, somatic embryos with two cotyledons developed a functional shoot apex with the tunica-corpus structure. In contrast, somatic embryos with one or fused cotyledon formed an abnormal shoot apex without the tunica-corpus structure or with non-dome shape in the inter-cotyledonary area. These results indicated that the variation of cotyledon in somatic embryos is closely related to procambial differentiation and shoot apical meristem development.
참고문헌 (12)
- Ammirato PV, "Organizational events during somatic embryogenesis. Plant Tissue and Cell Culture,", pp.57~81 (1987), Alan R Liss
- Buchheim JA, "Maturation of soybean somatic embryos and the transition to plantlet growth", Plant Physiol, 89, pp.768~775 (1989)
- Choi PS, "Somatic embryogenesis on cultures of Korean soybean (Glycine max L.) cultivars and effects of 2,4-dichlorophenoxyacetic acid", Kor J Plant Tiss Cult, 21, pp.7~13 (1994)
- Dos Santos AVP, "Origin and development of somatic embryos in Medicargo sativa L. (Alfalfa)", Protoplasma, 117, pp.107~115 (1983)
- Gray DJ, "Initiation and maintenance of long term somatic embryogenesis from anther and ovaries of Vitis longii “Microsperma”", Plant Cell Tiss Org Cult, 9, pp.73~80 (1987)
- Kageyama K, "Effects of sucrose concentration on morphology of somatic embryos from immature soybean cotyledons", Plant Tiss Cult Lett, 7, pp.108~110 (1990)
- Lee KS, "Structural diversity of cotyledons of somatic embryos from cultured cells in Aralia cordata Thumb", PhD, pp.1993
- Lee KS, "Somatic embryogenesis and structural aberrancy of embryos in tissue cultures of Aralia cordata Thumb", Kor J Plant Tiss Cult, 20, pp.77~84 (1993)
- Liu CM, "Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis", Plant Cell, 5, pp.621~630 (1993)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Raghavan V, "Role of 2,4-dichlorophenoxyacetic acid (2,4-D) in somatic embryogenesis on cultured zygotic embryos of Arabidopsis: cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2,4-D", Amer J Bot, 91, pp.1743~1756 (2004)
- Soh WY, "Plant regeneration from somatic embryos with anomalous cotyledons formed in cell cultures of Codonopsis lanceolata", Phytomorphology Golden Jubilee Issue, pp.327~336 (2001)