체세포배발생 형태에 따른 일천궁 기내식물체 재분화
Regeneration of Plant according to Somatic Embryogenesis Types in Cnidium officinale Makino
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초록
Background: The present study investigated the types and concentrations of plant hormones affecting somatic embryo induction from leaves, stems, and roots of Cnidium officinale Makino. Methods and Results: Somatic embryos could be induced using two methods: direct and indirect somatic embryogenesis. Direct somatic embryogenesis was induced when the whole explants were grown in a medium supplemented with cytokinin (e.g., kinetin), while indirect somatic embryogenesis was induced when the medium was supplement with auxin [eg., 2,4-dichlorophenoxyacetic acid (2,4-D)]. Both direct and indirect somatic embryos were mostly induced from leaves. The highest direct somatic embryo induction (95.8%) was obtained from leaf explants grwon on Murashige and Skoog (MS) medium supplemented with 0.5 - 1.0 ㎎/ℓ kinetin. The highest indirect somatic embryo induction (75.0%) was obtained from leaf explants grown on MS medium supplemented with 0.5 ㎎/ℓ 2,4-D. The induced somatic embryo could successfully germinate and acclimatize. Conclusions: Our results can be useful for establising a mass propagation protocol for C. officinale.
Abstract
Background: The present study investigated the types and concentrations of plant hormones affecting somatic embryo induction from leaves, stems, and roots of Cnidium officinale Makino. Methods and Results: Somatic embryos could be induced using two methods: direct and indirect somatic embryogenesis. Direct somatic embryogenesis was induced when the whole explants were grown in a medium supplemented with cytokinin (e.g., kinetin), while indirect somatic embryogenesis was induced when the medium was supplement with auxin [eg., 2,4-dichlorophenoxyacetic acid (2,4-D)]. Both direct and indirect somatic embryos were mostly induced from leaves. The highest direct somatic embryo induction (95.8%) was obtained from leaf explants grwon on Murashige and Skoog (MS) medium supplemented with 0.5 - 1.0 ㎎/ℓ kinetin. The highest indirect somatic embryo induction (75.0%) was obtained from leaf explants grown on MS medium supplemented with 0.5 ㎎/ℓ 2,4-D. The induced somatic embryo could successfully germinate and acclimatize. Conclusions: Our results can be useful for establising a mass propagation protocol for C. officinale.
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