더덕의 체세포배발생에서 전형층분화와 자엽수의 관계
Relationship of Cotyledon Number with Procambium Differentiation in Somatic Embryogenesis of Codonopsis lanceolata L.
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초록
더덕 자엽절편을 1 mg/L 2,4-D가 첨가된 MS기본배지에 배양하여 배발생캘러스를 얻었고, 호르몬이 첨가되지 않은 MS액체배지에서 구형기의 체세포배를 얻었다. 구형기에서 초기 심장형기로 발달할 때 자엽의 시원세포는 전형성층조직으로부터 분화되기 시작 하였으며, 하배축에서 관찰되는 원통형 전형성층대는 2개의 자엽을 형성할 경우 2개의 전형성층대가, 3개의 자엽은 3개의 전형성층대가, 4개의 자엽은 4개의 전형층대가 각각 독립적으로 분화되어 자엽절과 자엽부위까지 연결되어 있었다. 만약 구형기 체세포배에서 자엽시원세포가 원통형으로 분화될 경우 합생자엽을 갖는 체세포배가 형성되었다.
Abstract
Embryogenic callus was obtained from cotyledonary explants of Codonopsis lanceloata on Murashige & Skoog's medium supplemented with 1 mg/L 2,4-D. Suspension cultures of the embryogenic calli were grown on a shaker at 100 strokes/min, and then the calli were subcultured for 2 weeks in 2,4-D-free medium to produce somatic embryo. In somatic embryos at the globular stage, cotyledon initials began to differentiate themselves in the near distal end of the procambial strand. Dicotyledons, tricotyledon, tetracotyledon and fused cotyledon were differentiated from the distal ends of two, three, four and circular procambial strands, respectively. Nearly circular procambial strand in lower hypocotyls were independently differentiated into two, three, four procambial tissues at cotyledonary node and cotyledons to form somatic embryos with dicotyledon, tricotyledon, tetracotyledon. If the distal subepidermal cells of globular embryo exclusively became cotyledon initials, the torpedo or cotyledonary embryo was characterized by somatic embryos with fused cotyledon.
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