Morphological and histological characteristics of somatic embryo development of carrot ( Daucus carota L.)
Morphological and histological characteristics of somatic embryo development of carrot (Daucus carota L.)
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Embryogenic callus formation was induced from the cultures of hypocotyl explants of Daucus carota L. on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid. Liquid cultures of the embryogenic cells or clumps were maintained on a shaker at 120 strokes/min, and then the embryogenic cells or clumps were sub-cultured in hormone-free MS medium for two weeks to develop somatic embryos. Somatic embryos obtained from the liquid cultures were classified as somatic embryos (two cotyledons) and abnormal multi-cotyledons (three or four cotyledons). The differentiation of procambial tissue in the somatic embryos initiated with a circular shape in the hypocotyl region and then connected to the cotyledon nodes; the tissue differentiated into the cotyledon region to form two cotyledons with two procambial strands, three cotyledons with three procambial strands, and four cotyledons with four procambial strands. These results suggest that the differen- tiation of procambium in somatic embryos is closely related to the number of cotyledons formed.
Abstract
Embryogenic callus formation was induced from the cultures of hypocotyl explants of Daucus carota L. on Murashige and Skoog (MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid. Liquid cultures of the embryogenic cells or clumps were maintained on a shaker at 120 strokes/min, and then the embryogenic cells or clumps were sub-cultured in hormone-free MS medium for two weeks to develop somatic embryos. Somatic embryos obtained from the liquid cultures were classified as somatic embryos (two cotyledons) and abnormal multi-cotyledons (three or four cotyledons). The differentiation of procambial tissue in the somatic embryos initiated with a circular shape in the hypocotyl region and then connected to the cotyledon nodes; the tissue differentiated into the cotyledon region to form two cotyledons with two procambial strands, three cotyledons with three procambial strands, and four cotyledons with four procambial strands. These results suggest that the differen- tiation of procambium in somatic embryos is closely related to the number of cotyledons formed.
참고문헌 (18)
- Ammirato PV, "Plant Tissue and Cell Culture", pp.57~81 (1987), Alan R Liss
- Bhojwani SS, "Advances in developmental biology and biotechnology of higher plants", pp.110~127 (1993), Kor Soc Plant Tiss Cult
- Buchheim JA, "Maturation of soybean somatic embryos and the transition to plantlet growth", Plant Physiol, 89, pp.768~775 (1989)
- Choi PS, "Effects of carbohydrates and osmoticum on the somatic embryogenesis and cotyledon morphology of Codonopsis lanceolata L", J Plant Biotechnol, 47, pp.179~184 (2020)
- Choi PS, "Histological characteristics of somatic embryos in melon (Cucumis melo L.)", Kor J Plant Res, 26, pp.511~515 (2013)
- Choi PS, "Procambium differentiation and shoot apical meristem development in somatic embryos of soybean (Glycine max L.)", J Plant Biotechnol, 40, pp.55~58 (2013)
- Choi PS, "Somatic embryogenesis on cultures of Korean soybean(Glycine max L. )cultivars and effects of 2, 4-dichlorophenoxyacetic acid", Kor J Plant Tiss Cult, 21, pp.7~13 (1994)
- Choi PS, "Relationship of cotyledon number with procambium differentiation in somatic embryogenesis of Codonopsis lanceolata L", Kor J P lant Biotechnol, 32(2), pp.135~138 (2005)
- Garcia C, "Abnormalities in somatic embryogenesis caused by 2, 4-D : an overview", Plant Cell Tiss Org Cult, 137, pp.193~212 (2019)
- Gray DJ, "Initiation and maintenance of long term somatic embryogenesis from anther and ovaries of Vitis longii"Microsperma"", Plant Cell Tiss Org Cult, 9, pp.73~80 (1987)
- Kageyama K, "Effects of sucrose concentration on morphology of somatic embryos from immature soybean cotyledons", Plant Tiss Cult Lett, 7, pp.108~110 (1990)
- Lee KS, "Structural diversity of cotyledons of somatic embryos from cultured cells in Aralia cordata Thumb", PhD, pp.1993
- Lee KS, "Somatic embryogenesis and structural aberrancy of embryos in tissue cultures of Aralia cordata Thumb", Kor J Plant Tiss Cult, 20, pp.77~84 (1993)
- Lee YJ, "Effect of carbohydrates and osmotic agents on somatic embryogenesis and cotyledon morphology in carrot (Daucus carrot L.)", J Plant Biotechnol, 50, pp.89~95 (2023)
- Liu CM, "Auxin polar transport is essential for the establishment of bilateral symmetry during early plant embryogenesis", Plant Cell, 5, pp.621~630 (1993)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Raghavan V, "Role of 2, 4-dichlorophenoxyacetic acid(2, 4-D)in somatic embryogenesis on cultured zygotic embryos of Arabidopsis : cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2, 4-D", Amer J Bot, 91, pp.1743~1756 (2004)
- Soh WY, "Plant regeneration from somatic embryos with anomalous cotyledons formed in cell cultures of Codonopsis lanceolata", Phytomorphology Golden Jubilee, pp.327~336 (2001)