The Effects of Optimal Germination of Somatic Embryos Induced from Mature Cotyledon Explants of Panax ginseng C. A. Meyer by Gibberellic Acid
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초록
Somatic embryos on growth regulator-free medium can be produced directly from cotyledon explants of Panax ginseng C. A. Meyer. When the embryo developmental stage was torpedo and cotyledon, the germination rate of embryos was quite high on MS medium supplemented with gibberellic acid (GA3). However, the percentage of plantlet formation at the cotyledon stage was higher than that at the torpedo stage. This result demonstrates that the embryo at the cotyledon stage was the most appropriate for increasing germination by GA3. Embryos cultured on medium including four levels of GA3 concentrations (3, 5, 10, or 20 ㎎/ℓ ) showed all quite high germination rates (87-91%). When the well-developed embryos were continuously cultured on media including high concentrations of GA3 from 10 to 20 ㎎/ℓ , the percentage of formation of normal plantlets was lower than that seen under low concentrations from 3 to 5 ㎎/ℓ . This treatment of high concentrations resulted in shoots with abnormal shape. The optimal GA3 treatment provides a basis for the efficient method obtaining healthy plantlets derived from ginseng somatic embryos.
Abstract
Somatic embryos on growth regulator-free medium can be produced directly from cotyledon explants of Panax ginseng C. A. Meyer. When the embryo developmental stage was torpedo and cotyledon, the germination rate of embryos was quite high on MS medium supplemented with gibberellic acid (GA3). However, the percentage of plantlet formation at the cotyledon stage was higher than that at the torpedo stage. This result demonstrates that the embryo at the cotyledon stage was the most appropriate for increasing germination by GA3. Embryos cultured on medium including four levels of GA3 concentrations (3, 5, 10, or 20 ㎎/ℓ ) showed all quite high germination rates (87-91%). When the well-developed embryos were continuously cultured on media including high concentrations of GA3 from 10 to 20 ㎎/ℓ , the percentage of formation of normal plantlets was lower than that seen under low concentrations from 3 to 5 ㎎/ℓ . This treatment of high concentrations resulted in shoots with abnormal shape. The optimal GA3 treatment provides a basis for the efficient method obtaining healthy plantlets derived from ginseng somatic embryos.
참고문헌 (17)
- Arya S, "Rapid multiplication of adventitious somatic embryos of Panax ginseng", Plant Cell, Tissue and Organ Culture, 34, pp.57~162 (1993)
- Butenko RG, "Organogenesis and somatic embryogenesis in the tissue culture of Panax ginseng C. A. Meyer", Botanicheski Zhurnal, 7, pp.906~913 (1968)
- Chang WC, "In vitro flowering of embryoids derived from mature root callus of ginseng (Panax ginseng)", Nature, 284, pp.341~342 (1980)
- Choi KT, "Root and shoot formation in explant and callus derived from root and cotyledon of ginseng (Panax ginseng C. A. Meyer)", Korean Journal of Ginseng Science, 5, pp.35~40 (1981)
- Choi YE, "The role of excision on somatic embryogenesis from mature ginseng zygotic embryos", Phytomorphology, 46, pp.151~159 (1996)
- Choi YE, "Enhanced somatic single embryo formation by plasmolyzing pretreatment from cultured ginseng cotyledons", Plant Science, 130, pp.197~206 (1997)
- Choi YE, "Induction of somatic embryos by macrosalt stress from mature zygotic embryos of Panax ginseng", Plant Cell, Tissue and Organ Culture, 52, pp.177~181 (1998)
- Choi YE, "Regenerative ability of somatic single and multlple embryos from cotyledon of Korean ginseng on hormone-free medium", Plant Cell Reports, 17, pp.544~551 (1998)
- Choi YE, "High-efficiency plant production via direct somatic single embryogenesis from preplasmolysed cotyledons of Panax ginseng and possible dormancy of somatic embryos", Plant Cell Reports, 18, pp.493~499 (1999)
- Ok Tae Kim, "Optimization of Direct Somatic Embryogenesis from Mature Zygotic Embryos of Panax ginseng C. A. Meyer", Journal of Plant Biology(한국식물학회지), 49(5), pp.348~2 (2006), 한국식물학회
- Kishira H, "Micropropagation of Panax ginseng C. A. Meyer by somatic embryos", Acta Horticulturae, 319, pp.197~202 (1992)
- Langhansová L, "Polyethylene glycol and abscisic acid improve maturation and regeneration of Panax ginseng somatic embryos", Plant Cell Reports, 22, pp.725~730 (2004)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue", Physiologia Plantarum, 15, pp.473~497 (1962)
- Proctor JTA, "Ginseng: Industry, Botany, and Culture", Horticultural Reviews, 9, pp.187~236 (1987)
- Slepyan LI, "Panax ginseng C. A. Meyer as an object for introduction into tissue culture", Problems Pharmacology, 21, pp.198~203 (1967)
- Sun TP, "Molecular mechanism of gibberellin signaling in plants", Annual Review of Plant Biology, 55, pp.197~223 (2004)
- Zhou S, "High efficiency plant production of North American ginseng via somatic embryogenesis from cotyledon explants", Plant Cell Reports, 25, pp.166~173 (2006)