RAPD분석 기술을 이용한 토종도라지의 기원 분석
Differentiation of indigenous balloon flower (Platycodon grandiflorum DC.) germ lines in South Korea by using RAPD analyses
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참고문헌
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초록
The total production volume has been sharplyincreased from year 2008 in Gyeongnam province, SouthKorea by the policy of preservation and promotion ofindigenous balloon flower germ lines. In an attempt to assistthe Gyeongnam province's policy, in this study, we tried toestablish a technique to differentiate the indigenous balloonflower germ lines with those collected within South Koreaand China. Our preliminary results indicated that RAPDanalyses with five different primers exhibited high frequencyof polymorphic DNA bands up to 76.9% and phylogenetictree indicated that some of the indigenous lines can be easilydifferentiated with others. However, it was suggested thatmore advanced techniques such as single nucleotidepolymorphic markers need to be developed in particular, byusing extra-chromosomal DNA.
Abstract
The total production volume has been sharplyincreased from year 2008 in Gyeongnam province, SouthKorea by the policy of preservation and promotion ofindigenous balloon flower germ lines. In an attempt to assistthe Gyeongnam province's policy, in this study, we tried toestablish a technique to differentiate the indigenous balloonflower germ lines with those collected within South Koreaand China. Our preliminary results indicated that RAPDanalyses with five different primers exhibited high frequencyof polymorphic DNA bands up to 76.9% and phylogenetictree indicated that some of the indigenous lines can be easilydifferentiated with others. However, it was suggested thatmore advanced techniques such as single nucleotidepolymorphic markers need to be developed in particular, byusing extra-chromosomal DNA.
참고문헌 (14)
- Doyle JJ, "A rapid DNA isolation procedure for small quantities of fresh tissue", Phytochemica Bulletin, 19, pp.11~15 (1987)
- Lee YH, "Survey on cultural environment and soil morphological characteristics of Platycodon grandiflorum", Kor J Soil Sci fertilizer, 32, pp.215~222 (1999)
- Lee ST, "Crude saponin contents of Platycodon grandiflorum (Jacq.) A. DC", Kor J Med Crop, 7, pp.172~176 (1999)
- 이철호, "도라지 재배지역 및 재배년수에 따른 토양의 화학적 특성변화", 한국약용작물학회지, 18(4), pp.273~279 (2010), 한국약용작물학회
- Mabberley DJ, "The Plant Book", pp.461~ (1987)
- 박춘근, "Genetic Diversity and DNA Polymorphism in Platycodon grandiflorum DC. Collected from East-Asian Area", 한국약용작물학회지, 13(2), pp.115~120 (2005), 한국약용작물학회
- 박춘근, "청도라지와 백도라지의 구분을 위한 SCAR 마커 개발", 한국약용작물학회지, 15(1), pp.1~5 (2007), 한국약용작물학회
- Rohlf FJ, "NTSYS-pc numerical taxonomy and multivariate analysis system version 2.1. Owner manuel", ~ (2000)
- Seong JD, "Root yield and saponin content in different soil texture of Platycodon grandiflorum A. DC", Kor J Med Crop, 7, pp.282~287 (1999)
- Shi FH, "The performance of agronomic traits in F1 crossing combinations derived from male sterile line of (Platycodon grandiflorum)", Zhong Yao Cai, 34, pp.1815~1818 (2011)
- Shon MY, "Chemical compositions and physiological activities of Doraji (Platycodon grandiflorum)", J Kor Soc Food Sci Nutr, 30, pp.717~720 (2001)
- Song JY, "Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum (Campanulaceae)", Genet Mol Res, 11, pp.3263~3266 (2012)
- Tada A, "Studies on the saponins of the root of Platycodon grandiflorum A. DeCandolle. I. Isolation and the structure of platycodon D", Cheml Pharm Bull, 23, pp.2965~2972 (1975)
- Lee, CB, "A picture book of the Korean flora", pp.725~ (1980), Hyangmoon Co