Development of a Specific SNP Marker to Identify the Medicinal Plants P. grandiflorum
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The balloon flower (Platycodon grandiflorum A. DC.) is a medicinal and perennial flowering plant. Jangback is an important white-flowertype balloon flower cultivar registered in South Korea, but no molecular marker was available to differentiate it from other white-flowerlines. Therefore, we evaluated five P. grandiflorum white-flower lines and identified a single nucleotide polymorphism (SNP) derivedfrom the chloroplast TrnL-F genomic sequence that specifically differentiated Jangback from the other four genotypes. Cultivar identificationwas achieved by detecting allelic variations of the SNP using amplification refractory mutation system-polymerase chain reaction(ARMS-PCR) analysis and high resolution melting (HRM) curve analysis. The present study describes a rapid and reliable method toauthenticate the medicinally and economically valuable white-flower Jangback cultivar. Our results indicate that the plastid TrnL-F regionprovides for marker assisted identification and selection in intraspecific polymorphism studies, thereby the identified SNP marker providesa robust tool along with ARMS-PCR and HRM curve analysis for rapid and efficient identification of the medicinally valuable Jangbackcultivar.
Abstract
The balloon flower (Platycodon grandiflorum A. DC.) is a medicinal and perennial flowering plant. Jangback is an important white-flowertype balloon flower cultivar registered in South Korea, but no molecular marker was available to differentiate it from other white-flowerlines. Therefore, we evaluated five P. grandiflorum white-flower lines and identified a single nucleotide polymorphism (SNP) derivedfrom the chloroplast TrnL-F genomic sequence that specifically differentiated Jangback from the other four genotypes. Cultivar identificationwas achieved by detecting allelic variations of the SNP using amplification refractory mutation system-polymerase chain reaction(ARMS-PCR) analysis and high resolution melting (HRM) curve analysis. The present study describes a rapid and reliable method toauthenticate the medicinally and economically valuable white-flower Jangback cultivar. Our results indicate that the plastid TrnL-F regionprovides for marker assisted identification and selection in intraspecific polymorphism studies, thereby the identified SNP marker providesa robust tool along with ARMS-PCR and HRM curve analysis for rapid and efficient identification of the medicinally valuable Jangbackcultivar.
참고문헌 (20)
- Fu WW, "Five new triterpenoid saponins from the roots of Platycodon grandiflorum", Chem. Pharm. Bull, 54, pp.557~560 (2006)
- Han EH, "Development of molecular markers, based on chloroplast and ribosomal DNA regions, to discriminate three popular medicinal plant species, Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum", Mol. Biol. Rep, 43, pp.323~332 (2016)
- Han EH, "Establishment of tissue culture and acclimation of white balloon flower (Platycodon grandiflorum DC. cv. Jangback) for the raising of in vitro propagated seedlings", J. Plant Biotechnol, 41, pp.134~139 (2014)
- Harris SA, "Chloroplast DNA and biosystematics : The effects of intraspecific diversity and plastid transmission", Taxon, 40, pp.393~412 (1991)
- Kim JH, "Diversity and evolution of major Panax species revealed by scanning the entire chloroplast intergenic spacer sequences", Genet. Res. Crop Evol, 60, pp.413~425 (2013)
- Kim TW, "Differentiation of indigenous balloon flower (Platycodon grandiflorum DC.) germ lines in South Korea by using RAPD analyses", J. Plant Biotechnol, 41, pp.19~25 (2014)
- Lee GA, "Analysis of population structure and genetic diversity in balloon flower (Platycodon grandiflorum (Jacq.) A. DC.) germplasm using simple sequence repeat (SSR) markers", J. Crop Sci. Biotechnol, 15, pp.281~287 (2012)
- Lee JY, "Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots", J. Ethnopharmacol, 93, pp.409~415 (2004)
- Lee SW, "Development of molecular markers for the differentiation of Angelica gigas Jiri line by using ARMS-PCR analysis", J. Plant Biotechnol, 48, pp.26~33 (2021)
- Lee SW, "Development of specific SNP molecular marker from Thistle in the DNA sequences of chloroplast ITS region", J. Life Sci, 29, pp.524~529 (2019)
- McCauley DE, "The use of chloroplast DNA polymorphism in studies of gene flow in plants", Trend. Ecol. Evol, 10, pp.198~202 (1995)
- McDade LA, "Phylogenetic relationships among Acantheae(Acanthaceae) : Major lineages present contrasting patterns of molecular evolution and morphological differentiation", Syst. Bot, 30, pp.834~862 (2005)
- Newton CR, "Analysis of any point mutation in DNA", The amplification refractory mutation system (ARMS). Nucl. Acids Res, 17, pp.2503~2516 (1989)
- Park CG, "Development of SCAR marker for discriminating between violet flowered lines and white flowered lines in Chinese bellflower (Platycodon grandiflorum A.)", Kor. J. Med. Crop Sci, 15, pp.1~5 (2007)
- Pyke KA, "Plastid division and development", Plant Cell, 11, pp.549~556 (1999)
- Reboud X, "Organelle inheritance in plants", Heredity, 72, pp.132~140 (1994)
- Song JY, "Development and characterization of 22 polymorphic microsatellite markers for the balloon flower Platycodon grandiflorum(Campanulaceae)", Genet. Mol. Res, 11, pp.3263~3266 (2012)
- Sun H, "A simple and rapid technique for the authentication of the ginseng cultivar, Yunpoong, using an SNP marker in a large sample of ginseng leaves", Gene, 487, pp.75~79 (2011)
- Ward J, "A molecular identification system for grasses : A novel technology for forensic botany", Forensic. Sci. Int, 152, pp.121~131 (2005)
- Wolfe AD, "Recombination, heteroplasmy, haplotype polymorphism, and paralogy in plastid genes : implications for plant molecular systematics", Syst. Bot, 29, pp.1011~1120 (2004)