멸종위기종 둥근잎꿩의비름 (Hylotelephium ussuriense (Kom.) H. Ohba)의 기내 증식
In vitro propagation of endangered species, Hylotelephium ussuriense (Kom.) H. Ohba
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
To establish the system of in vitro plant regeneration,the different explants (stem with axillary bud and stemwithout axillary bud) of Hylotelephium ussuriense werecultured on the Murashige and Skoog’s medium containing6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in thestem with axillary bud explants than the stem without axillarybud explants, and was the best on MS medium containing 3.0mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growthwas not significantly treated on MS and sucrose. Totalchlorophyll contents under ventilation treatment were higherthan those in control (non-ventilation). This in vitro propagationprotocol will be useful for conservation and mass propagationof this endangered plant.
Abstract
To establish the system of in vitro plant regeneration,the different explants (stem with axillary bud and stemwithout axillary bud) of Hylotelephium ussuriense werecultured on the Murashige and Skoog’s medium containing6-benzylaminopurine (BA) and indolebutyric acid (IBA). The adventitious shoot induction was more effective in thestem with axillary bud explants than the stem without axillarybud explants, and was the best on MS medium containing 3.0mg/L BA and 0.01 mg/L IBA. Frequency of plantlet growthwas not significantly treated on MS and sucrose. Totalchlorophyll contents under ventilation treatment were higherthan those in control (non-ventilation). This in vitro propagationprotocol will be useful for conservation and mass propagationof this endangered plant.
참고문헌 (33)
- 안정호, "돌나물 엽절편으로부터 캘러스 형성 및 식물체 분화에 미치는 생장조절물질의 영향", Journal of Plant Biotechnology, 31(1), pp.25~29 (2004), 한국식물생명공학회
- Kee-Wha Bae, "Callus induction and plant regeneration of Iris dichotoma Pall. in endangered species", Journal of Plant Biotechnology, 39(3), pp.182~188 (2012), 한국식물생명공학회[DOI]
- 배기화, "홍경천(Rhodiola rosea L.)의 액아배양을 통한 다신초 유도 및 기내 대량증식", Journal of Plant Biotechnology, 39(2), pp.114~120 (2012), 한국식물생명공학회[DOI]
- Bae KH, "Factors affecting fruit baring in natural habitat and in vitro culture of zygotic embryos of Cypripedium japonicum", Prog Ornament Plant, 11, pp.146~152 (2013)
- Bae KH, "Structural changes of seed coats and stimulation of in vitro germination of fully mature seeds of Cypripedium macranthos Swartz by NaOCl pretreatment", Prog Ornament Plant, 10, pp.107~113 (2010)
- 배기화, "Effects of NaOCl treatment on in vitro germination of seeds of a rare endemic plant, Oreorchis coreana Finet", Journal of Plant Biotechnology, 40(1), pp.43~48 (2013), 한국식물생명공학회
- Bae KH, "In vitro germination and plantlet conversion from the culture of fully mature seeds of Cypripedium guttatum Swartz", Prog Ornament Plant, 9, pp.160~165 (2009)
- Chen TH, "Somatic embryogenesis and plant regeneration from cultures inflorescence of Oryza sativa L", Plant Cell Tissue Organ Cult, 21, pp.111~117 (1985)
- Chung TH, "Korean flora (Herb part)", pp.283~ (1974), Academybook
- Dumas, E, "In vitro rooting of micropropagated shoots from juvenile and mature Pinus pinaster explants influence of activated charcoal", Plant Cell, Tissue and Org. Cult, 40, pp.231~235 (1995)
- Eymar, E, "Effect of activated charcoal and 6-benzyladenine on in vitro nitrogen uptake by Lagerstroemia indica", Plant Cell, Tissue and Org. Cult, 63, pp.57~65 (2000)
- Gabriela, F, "Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets", In Vitro Cellular and Developmental Biology, 41, pp.69~76 (2005)
- Gonzlez A, "Ethylene involvement in in vitro organogenesis and plant growth of Populus tremula L", Plant Growth Regul, 22, pp.1~6 (1997)
- Jang JG, "Sanyacho Dongeubogam", pp.156~157 (2001), Academy press
- Jeong JH, "Growth and flowering response of potted Sedum rotundifolium to low temperature, photoperiod and GA3", J Kor Soc Hort Sci, 40, pp.761~764 (1999)
- Jeong JH, "Critical daylength for flower bud formation and development of Sedum rotundifolium", J Basic and Life Res Sci, 1, pp.219~222 (2001)
- 정정학, "둥근잎꿩의비름의 화아형성과 화아발달에 필요한 유도일장기간", 화훼연구, 11(2), pp.225~228 (2003), 한국화훼학회
- 김지아, "Eucalyptus pellita의 기내 줄기생장에 미치는 LEDs(Light-emitting diodes) 및 환기처리 효과", 한국임학회지, 95(6), pp.716~722 (2006), 한국임학회
- 권혜경, "둥근잎꿩의비름(Sedum rotundifolium D. Lee)의 식물체 재분화에 미치는 식물생장조절제의 영향", Journal of Plant Biotechnology, 37(1), pp.84~88 (2010), 한국식물생명공학회
- Lee, YM, "Illustrated rare and endangered species in Korea", pp.41~ (1997), Korea National Arboretum
- Lichtenthaler HK, "Chlorophylls and carotenoids: pigments of photosynthetic biomembranes", Meth Enzymol, 148, pp.350~382 (1987)
- Magdalena, K, "Morphogenesis of Lilium martagon L. explants in callus culture", Acta Biologia Cracoviensia Series Botanica, 47, pp.65~73 (2005)
- Ministry of Environment, "Enforcement decrees drafted for the Wildlife Protection Act", pp.83~ (2005)
- 문흥규, "액아배양에 의한 유묘 및 성숙 히어리나무의 기내변식", Journal of Plant Biotechnology, 29(2), pp.117~121 (2002), 한국식물생명공학회
- Moon HK, "Micropropagation of a rare species, Abeliophyllum distichum Nakai. via axillary bud culture", Kor J Plant Tiss Cult, 26, pp.133~136 (1999)
- 문흥규, "희귀 및 멸종위기 식물 덩굴용담의 기내생장에 미치는 광질 및 환기효과", Journal of Plant Biotechnology, 35(3), pp.215~221 (2008), 한국식물생명공학회
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~479 (1962)
- Pan, MJ, "The use of charcoal in in vitro culture-a review", Plant Growth Regulator, 26, pp.155~163 (1998)
- Ryu JH, "Induction of haploid plants by anther culture in sesame (Sesamum indicum L.)", Kor J Plant Tissue Cult, 19, pp.171~177 (1992)
- Seo, CB, "Ilustrated Flora of Korea", pp.206~209 (1979), Hyangmoonsa
- Takayama, S, "Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions", Physiology Plantarum, 46, pp.184~190 (1979)
- Yoon ES, "Effect of plant growth regulators on plant regeneration from lesf and stem explants cultures of Sedum erythrostichum Miq", Kor J plant Tissue Cult, 24, pp.285~289 (1997)
- Zobayed SMA, "Photoautotrophic (sugar-fee medium) micropropagation as a new propagation and transplant production system", pp.147~186 (2005), Springer