자운영(Astragalus sinicus L.) 배축절편으로부터 식물체 재생
Plant regeneration from hypocotyls explants of Astragalus sinicus L.
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초록
To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog’s (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into ½ MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.
Abstract
To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog’s (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into ½ MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.
참고문헌 (23)
- Barwale UB, "Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node", Theor. Appl. Genet, 72, pp.423~428 (1986)
- Baslma D, "TDZ-induced plant regeneration in Astragalus cicer L", African J of Biotechnol, 7, pp.955~959 (2008)
- Cho DY, "Morphological observation of somatic embryogenesis in leaf explants cultures of Bupleurum falcatum L", Korean J Plant Tiss Cult, 22, pp.241~260 (1995)
- Cho DY, "Plant regeneration from somatic embryo with structural diversity from leaf explants cultures of Osteicum koreanum Kitagawa", Korean J Plant Tiss Cult, 25, pp.51~56 (1998)
- Cho HJ, "Tryptophan Synthesis in a Forage Legume Astragalus sinicus by Expressing the Tobacco Feedback-Insensitive Anthranilate synthase (ASA2)Gene", Plant Physiol, 123, pp.1069~1076 (2000)
- Cho HJ, "Improved shoot regeneration protocol for hairy roots of the legume Astragalus sinicus", Plant Cell, Tiss Org Cult, 69, pp.259~269 (2002)
- 최필선, "대두 재조합순계주에서 고빈도 체세포배발생능 계통 조사", Journal of Plant Biotechnology, 29(2), pp.135~138 (2002), 한국식물생명공학회
- Erisen S, "Callus induction and plant regeneration of endemic Astragalus nezaketae in Turkey", Electron of Biotechnol (2010)[DOI]
- Gill R, "Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron", Can J Bot, 70, pp.1186~1192 (1992)
- Hung CY, "Development of an efficient plant regeneration system for the selenium-hyperaccumulator Astragalus recemosus and the non-accumulator Astragalus canadensis", HortScience, 43, pp.2138~2142 (2008)
- Hou SW, "High frequency plant regeneration from Astragalus melilotoides hypocotyl and stem explants via somatic embryogenesis and organogenesis", Plant Cell Tiss Org Cult, 79, pp.95~100 (2004)
- 김상열, "Physical Dormancy in Seeds of Chinese Milk Vetch (Astragalus sinicus L.) from Korea", 한국작물학회지, 53(4), pp.421~426 (2008), 한국작물학회
- 김상열, "토양속에서 자운영 종자의 휴면성 및 종자활력 변화", 한국작물학회지, 55(1), pp.76~82 (2010), 한국작물학회
- Luo JP, "Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall", Plant Cell Rep, 17, pp.313~317 (1998)
- Luo JP, "High frequency somatic embryogenesis and plant regeneration in callus cultures of Astragalus adsurgens Pall", Plant Sci, 143, pp.93~99 (1999)
- Malik KA, "Regeneration in Phaseoulus vulgaris seedling by N6-benzylaminopurine and thidiazuron", Planta, 186, pp.384~389 (1992)
- Mirci S, "High frequency of adventitious shoot regeneration from leaf and leaf petiol ofendemic Astragalus polemoniacus Bunge", Selçuk University Agricultural Faculty Publications, 18, pp.31~34 (2004)
- Murashige T, "A revised medium for rapid growth and bioassay with tobacco tissue culture", Physiol Plant, 15, pp.473~479 (1962)
- Murooka Y, "Formation of root nodules by Rhizobium huakuii biovar rengei bv. nov. in Astragalus sinicus cv", Japan J. Ferment Bioeng, 76, pp.38~44 (1993)
- Soh WY, "Multicotyledonary structure of somatic embryo formed cell cultures of Daucus carota L", Korean J Bot, 39, pp.71~79 (1996)
- Turgut-Kara N, "In vitro plant regeneration from embryogenic cell suspension culture of Astragalus chrysochlorus (Leguminoseae)", African Journal of Biotechnology, 7, pp.1250~1255 (2008)
- Wright MS, "Plant regeneration by organogenesis in Glycine max", Plant Cell Rep, 5, pp.150~154 (1986)
- Yasue T, "Development of Utilization of Chinese Milk Vetch (Astragalus sinicus L.) as a honey plant", Honeybee Sci, 9, pp.57~60 (1985)