Development of a green soybean line with a green cotyledon and a tetra null genotype for P34, lectin, Kunitz trypsin inhibitor, and lipoxygenase proteins
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Soybean (Glycine max (L.) Merr.) varieties with a green seed coat and cotyledon have long been cultivated for their high levels of lutein, which is considered beneficial for eye health. However, mature soybean seeds also contain major antinutritional and allergenic factors, including P34, Kunitz trypsin inhibitor (KTI), lectin, and lipoxygenase proteins. The objective of this research was to develop a new soybean line with a green cotyledon and a green seed coat, and without P34, KTI, lectin, and lipoxygenase proteins. A breeding population was developed using two cultivars and three germplasms. Seventy-four F2 seeds with a green cotyledon and a green seed coat were obtained. Twenty-four F2 seeds with a tetra null genotype (p34p34-titi-lele-lox1 lox1lox2lox2lox3lox3) were selected and planted in a greenhouse. One F2 plant that exhibited suitable agronomic traits was selected. The SDS-PAGE and western blot methods were used to determine the absence (tetra null genotype) of P34, KTI, lectin, and lipoxygenase proteins in random F3 seeds from the F2 plant selected. The color of the cotyledon and seed coat for the new selection line was green. The stem height was 72 cm and the 100-seed weight was 21.5 g. The new line obtained in this research could be bred into a soybean variety with a green cotyledon, a green seed coat, and fewer allergenic and antinutritional properties.
Abstract
Soybean (Glycine max (L.) Merr.) varieties with a green seed coat and cotyledon have long been cultivated for their high levels of lutein, which is considered beneficial for eye health. However, mature soybean seeds also contain major antinutritional and allergenic factors, including P34, Kunitz trypsin inhibitor (KTI), lectin, and lipoxygenase proteins. The objective of this research was to develop a new soybean line with a green cotyledon and a green seed coat, and without P34, KTI, lectin, and lipoxygenase proteins. A breeding population was developed using two cultivars and three germplasms. Seventy-four F2 seeds with a green cotyledon and a green seed coat were obtained. Twenty-four F2 seeds with a tetra null genotype (p34p34-titi-lele-lox1 lox1lox2lox2lox3lox3) were selected and planted in a greenhouse. One F2 plant that exhibited suitable agronomic traits was selected. The SDS-PAGE and western blot methods were used to determine the absence (tetra null genotype) of P34, KTI, lectin, and lipoxygenase proteins in random F3 seeds from the F2 plant selected. The color of the cotyledon and seed coat for the new selection line was green. The stem height was 72 cm and the 100-seed weight was 21.5 g. The new line obtained in this research could be bred into a soybean variety with a green cotyledon, a green seed coat, and fewer allergenic and antinutritional properties.
참고문헌 (25)
- Baker EC, "Heat inactivation of trypsin inhibitor, lipoxygenase and urease in soybeans : effect of acid and base additives", J Am Oil Chem Soc, 50(5), pp.137~141 (1973)
- Bilyeu K, "Association of a four-base pair insertion in the P34 gene with the low-allergen trait in soybean", Plant Genome, 2, pp.141~148 (2009)
- Choi S, "Breeding of green soybean line with green cotyledon and triple null genotype for KTI, lectin and P34 proteins", J of Agriculture & Life Sci, 56(2), pp.1~6 (2022)
- Davies CS, "Genetic analysis of a null-allele for lipoxygenase-2 in soybean", Crop Sci, 26, pp.460~463 (1986)
- Goldberg RB, "An insertion sequence blocks the expression of a soybean lectin gene", Cell, 33, pp.465~475 (1983)
- Han E, "Inheritance of Kunitz Trypsin Inhibitor and P34 protein in soybean seed", Korean J Crop Sci, 57(1), pp.78~82 (2012)
- Herman EM, "Genetic modification removes an immunodominant allergen from soybean", Plant Physiology, 132, pp.36~43 (2003)
- Hildebrand DF, "Inheritance of Lipoxygenase-1 activity in soybean seeds", Crop Sci, 22, pp.851~853 (1982)
- Joseph LM, "Evaluation of Glycine germplasm for nulls of the immunodominant allergen P34", Gly m Bd 30k, 2006, pp.46(4), 1755~1763
- Kitamura K, "Genetic analysis of a null-allele for lipoxygenase-3 in soybean seeds", Crop Sci, 23, pp.924~927 (1983)
- Koo SC, "Proteomic analysis of seed storage proteins in low allergenic soybean accession", J Korean Soc Appl Biol Chem, 54(3), pp.332~339 (2011)
- Kunitz M, "Crystallization of a soybean trypsin inhibitor from soybean", Science, 101, pp.668~669 (1945)
- Ly S, "Breeding of green soybean strain with green cotyledon and tetra null genotype", J of Life Science, 33, pp.632~638 (2023)
- Miyake K, "Lectin-based food poisoning : a new mechanism of protein toxicity", PloS ONE Aug, 2(8), pp.e687~ (2007)
- Ogawa T, "Identification of the soybean allergenic protein, Gly m Bd 30K, with the soybean 34-kDa oil-body-associated protein", Biosci Biotechnol Biochem, 57, pp.1030~1033 (1993)
- Merrill) seeds with different seed coat colors, "Kor J of Agricultural Sci", 2019, 971~980
- Okamuro JK, "A mutant lectin gene is rescued from an insertion element that blocks its expression", The Plant Cell, 4, pp.1141~1146 (1992)
- Orf JH, "Inheritance of the absence of the Kunitz trypsin inhibitor in seed protein of soybeans", Crop Sci, 19, pp.107~109 (1979)
- Orf JH, "Inheritance of a soybean seed lectin", Crop Sci, 18, pp.899~900 (1978)
- Pan L, "The influences of soybean agglutinin and functional oligosaccharides on the intestinal tract of monogastric animals", International J of Molecular Sciences, 19, pp.554~ (2018)
- Rackis J, "Protease inhibitors: physiological properties and nutritional significance", Antinutrients and Natural Toxicants in Foods, pp.203~223 (1981)
- Rodrigues AA, "The science behind lutein", Toxicol Lett, 150(1), pp.57~83 (2004)
- Schmidt MA, "Breeding and characterrization of soybean triple null; a stack of recessive alleles of Kunitz trypsin inhibitor, soybean agglutinin, and P34 allergen nulls", Plant Breeding, 134, pp.310~315 (2015)
- Vagadia BH, "Inactivation methods of soybean trypsin inhibitor-A review", Trends in Food Science & Technology, 64, pp.115~125 (2017)
- Vasconcelos IM, "Antinutritional properties of plant lectins", Toxicon, 44, pp.385~403 (2004)