녹색종피 및 자엽과 KTI, Lectin, P34 단백질에 대한 Triple Null 유전자형을 가진 콩 육종
Breeding of Green Soybean Line with Green Cotyledon and Triple Null Genotype for KTI, Lectin and P34 Proteins
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콩 [Glycine max (L.) Merr.]은 단백질, 지방, 탄수화물의 3대 영양소와 다양한 기능성 성분의 주요 공급원이다. 녹색종피와 자엽을 가진 콩품종은 눈건강에 유익한 루테인 성분을 많이 함유하고 있지만, 항영양성분으로 알려진 쿠니츠트립신인히비터 단백질, 렉틴 단백질 및 P34 단백질을가지고 있어 품질과 기능성을 저하시킨다. 이러한 성분이 유전적으로 제거된 품종의 육성이 필요하다. 녹색종피와 자엽을 가지고 있지만, 쿠니츠트립신인히비터, 렉틴, P34의 3가지 단백질이 모두 없는 triple null 유전자형을 가진 콩 계통을 육성하기 위하여 본 연구가 진행되었다. 4개의 유전자원(Gaechuck#2, PI548392, PI567476, Seonyack)을 이용하여 창성된 두 모본의 교배로 육종집단이 창성되었다. Western blot 기술을 이용하여쿠니츠트립신인히비터, 렉틴 및 P34 단백질의 존재여부를 확인하였다. 녹색종피 및 자엽과 쿠니츠트립신인히비터, 렉틴, P34의 3가지 단백질이모두 없는 triple null 유전자형 (titilelep34p34)을 가진 6개의 F2 종자가 선발되었다. F2 식물체 세대와 농업형질 평가를 통하여 한 개의 계통이선발되었으며 F4 종자에서 3가지 단백질에 대한 유전적 고정을 확인하였다. 선발 계통의 경장은 73 cm 정도였으며 백립중은 19.5 g으로 중립에속하였고 종피색은 녹색이고 제색은 검정색이며 성숙 자엽색은 녹색이었다. 본 연구를 통하여 선발된 계통은 성숙 콩 종실에서 품질과 기능성을저하시키는 쿠니츠트립신인히비터, 렉틴, P34의 3가지 단백질이 모두 없으며 녹색종피와 자엽을 가진 유색콩 품종 육성을 위한 재료로 이용될수 있을 것으로 사료되었다.
Abstract
Soybean [Glycine max (L.) Merr.] is an excellent sources of protein, oil and carbohydrates for humans and animals. Green seed-coat with green cotyledon soybean is known to contain a lot of lutein component which is good for eye health. Also, soybean seeds contain anti-nutritional factors such as Kunitz Trypsin Inhibitor (KTI), lectin and P34 proteins. The genetic removal of these components will improve the nutritional value of green seed soybean. The objective of this study was to breed new soybean line with green seed coat, green cotyledon and triple null genotype (titilelep34p34) for KTI (titi), lectin (lele) and P34 proteins (p34p34). Two new parents were developed to create breeding population using four germplasms. Presence and absence of KTI, lectin and P34 protein was detected by Western Blot technique. Six F2 seeds with green seed coat, green cotyledon and triple null genotype (titilelep34p34) were selected. One F3 plant strain with green seed coat, green cotyledon and triple null genotype (titilelep34p34) was selected. A new strain has purple flower and black hilum. Stem height of new strain was 73 cm and 100-seed weight was 19.5 g. A new soybean line obtained through this research will be used to improve new triple null cultivar (free of KTI, lectin and P34 proteins) with green seed coat, green cotyledon and high nutrition value.
참고문헌 (23)
- Bilyeu K, "Association of a four-base pair insertion in the P34 gene with the low-allergen trait in soybean", Plant Genome, 2, pp.141~148 (2009)
- 최상우, "Breeding of Black Soybean Line with ti and le Allele", Plant Breeding and Biotechnology, 4(2), pp.170~175 (2016), 한국육종학회[DOI]
- 정종일, "A New Soybean Cultivar “Gaechuck#2” : Yellow Soybean Cultivar with Lipoxygenase2,3-free and Kunitz Trypsin", 한국육종학회지, 41(4), pp.612~615 (2009), 한국육종학회
- Goldberg RB, "An insertion sequence blocks the expression of a soybean lectin gene", Cell, 33, pp.465~475 (1983)
- Helm RM, "Mutantional analysis of the IgE-binding epitopes of P34", Gly m Bd 30K, 2000, pp.105, 378~384
- Herman EM, "Genetic modification removes an immunodominant allergen from soybean", Plant Physiology, 132, pp.36~43 (2003)
- Hymowitz T, "Inheritance of a trypsin inhibitor variant in seed protein of soybeans", Crop Sci, 12, pp.197~198 (1972)
- Joseph LM, "Evaluation of Glycine germplasm for nulls of the immunodominant allergen P34", Gly m Bd 30k, 2006, pp.46(4), 1755~1763
- Krogdahl A, "Soybean proteinase inhibitors and human proteolytic enzymes : Selective inactivation of inhibitors by treatment with human gastric juice", The J. Nutrition, 111, pp.2045~2051 (1981)
- Kunitz M, "Crystallization of a soybean trypsin inhibitor from soybean", Science, 101, pp.668~669 (1945)
- Liener IE, "Possible adverse effects of soybean anticarcinogens", J. Nutr, 125, pp.744~750 (1995)
- Miyake K, "Lectin-based food poisoning : A new mechanism of protein toxicity", PloS One, 8, pp.e687~ (2007)
- Monma M, "Carotenoid components in soybean seeds varying with seed color and maturation stage", Biosci. Biotechnol. Biochem, 58, pp.926~930 (1994)
- Ogawa T, "Identification of the soybean allergenic protein, Gly m Bd 30K, with the soybean 34-kDa oil-body-associated protein", Biosci. Biotechnol. Biochem, 57, pp.1030~1033 (1993)
- Orf J, "Inheritance of a soybean seed lectin 1", Crop Science, 18, pp.899~900 (1978)
- Orf JH, "Inheritance of the absence of the Kunitz trypsin inhibitor in seed protein of soybeans", Crop Sci, 19, pp.107~109 (1979)
- Pan L, "The influences of soybean agglutinin and functional oligosaccharides on the intestinal tract of monogastric animals", International J. of Molecular Sciences, 19, pp.554~ (2018)
- Schmidt MA, "Breeding and characterrization of soybean triple null; A stack of recessive alleles of Kunitz trypsin inhibitor, soybean agglutinin, and P34 allergen nulls", Plant Breeding, 134, pp.310~315 (2015)
- 성미경, "Inheritance of dlm and ti genes in Soybean", Plant Breeding and Biotechnology, 1(1), pp.9~13 (2013), 한국육종학회[DOI]
- Vasconcelos IM, "Antinutritional properties of plant lectins", Toxicon, 44, pp.385~403 (2004)
- Vodkin LO, "A lectin gene insertion has the structural features of a transposable element", Cell, 34, pp.1023~1031 (1983)
- Vodkin LO, "Isolation and characterization of mRNAs for seed lectin and Kunitz trypsin inhibitor in soybeans", Plant Physiol, 68, pp.766~771 (1981)
- Yamanishi R, "Reduction of the soybean allergenicity by the fermentation with Bacillus natto", Food Science and Technology International, Tokyo, 1(1), pp.14~17 (1995)