The development of new soybean strain with ti and cgy1 recessive allele
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Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrate, isoflavone and other various nutrients for humans and animals. However, there are anti-nutritional factors in the raw mature soybeans. Kunitz trypsin inhibitor (KTI) protein and stachyose are the main anti-nutritional factors in soybean seed. The α′-subunit of β-conglycinin protein exhibit poor nutritional and food processing properties. The genetic removal of the KTI and α′-subunit proteins will improve the nutritional value of the soybean seed. The objective of this research was to develop a new soybean strain with KTI and α′-subunit protein free (titicgy1cgy1 genotype) and proper agronomic traits. A breeding population was developed from the cross of the Bl-1 and 15G1 parents. A total of 168 F2 seeds from the cross of the BL-1 and 15G1 parents were obtained. The segregation ratios of 9: 3: 3: 1 (104 Ti_Cgy1_: 30 Ti_cgy1cgy1: 21 cgy1cgy1Ti_: 13 titicgy1cgy1) between the Ti and Cgy1 genes in the F2 seeds were observed (χ 2= 5.12, P=0.5–0.10). Two F4 plant strains with proper agronomical traits and titicgy1cgy1 genotype (free of both KTI and α′ -subunit protein) were selected and harvested. 2 strains (S1 and S2) had yellow seed coats and hilum. The plant height of the S1 strain was 65 centimeters. The 100-seed weight was 29.2 g. The plant height of the S2 strain was 66 centimeters and 100-seed weight was 26.2 g. The two strains selected in this research will be used to improve the new cultivar that will be free of the KTI and α′-subunit proteins.
Abstract
Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrate, isoflavone and other various nutrients for humans and animals. However, there are anti-nutritional factors in the raw mature soybeans. Kunitz trypsin inhibitor (KTI) protein and stachyose are the main anti-nutritional factors in soybean seed. The α′-subunit of β-conglycinin protein exhibit poor nutritional and food processing properties. The genetic removal of the KTI and α′-subunit proteins will improve the nutritional value of the soybean seed. The objective of this research was to develop a new soybean strain with KTI and α′-subunit protein free (titicgy1cgy1 genotype) and proper agronomic traits. A breeding population was developed from the cross of the Bl-1 and 15G1 parents. A total of 168 F2 seeds from the cross of the BL-1 and 15G1 parents were obtained. The segregation ratios of 9: 3: 3: 1 (104 Ti_Cgy1_: 30 Ti_cgy1cgy1: 21 cgy1cgy1Ti_: 13 titicgy1cgy1) between the Ti and Cgy1 genes in the F2 seeds were observed (χ 2= 5.12, P=0.5–0.10). Two F4 plant strains with proper agronomical traits and titicgy1cgy1 genotype (free of both KTI and α′ -subunit protein) were selected and harvested. 2 strains (S1 and S2) had yellow seed coats and hilum. The plant height of the S1 strain was 65 centimeters. The 100-seed weight was 29.2 g. The plant height of the S2 strain was 66 centimeters and 100-seed weight was 26.2 g. The two strains selected in this research will be used to improve the new cultivar that will be free of the KTI and α′-subunit proteins.
참고문헌 (15)
- Cregan PB, "An integrated genetic linkage map of the soybean", Crop Sci, 39, pp.1464~1490 (1999)
- Hajika M, "Dominant inheritance of a trait lacking β-conglycinin detected in a wild soybean line", Breeding Science, 48, pp.383~386 (1998)
- Hayashi M, "Identification of AFLP markers tightly linked to the gene for deficiency of the 7S globulin in soybean seed and characterization of abnormal phenotypes involved in the mutation", Breeding Science, 50, pp.123~129 (2000)
- Hildebrand DF, "Inheritance of anacid phosphatase and its linkage with the Kunitz trypsin inhibitor seed protein of soybeans", Crop Sci, 20, pp.83~85 (1980)
- Hymowitz T, "Inheritance of a trypsin variant in seed protein of soybeans", Crop Sci, 12, pp.197~198 (1972)
- Kiang YT, "Mapping three protein loci on a soybean chromosome", Crop Sci, 27, pp.44~46 (1987)
- Kitamura K, "Mutant strains with low level of subunits of 7S globulins in soybean seed", Japan J Breed, 31, pp.353~359 (1981)
- Kitamura K, "Inheritance of alleles for Cgy1 and Gy4 storage protein genes in soybean", Theor Appl Genet, 68, pp.253~257 (1984)
- Koshiyama I, "Chemical and physical properties in soybean globulins", Cereal Chem, 45, pp.394~404 (1968)
- Kunitz M, "Crystallization of a trypsin inhibitor from soybean", Science, 101, pp.668~669 (1945)
- Ogawa TE, "Genetic improvement of seed storage proteins using three variant alleles of 7S globulin subunits in soybean", Japan J Breed, 39, pp.137~147 (1989)
- Orf JH, "Inheritance of the absence of the trypsin inhibitor in seed protein of soybeans", Crop Sci, 19, pp.107~109 (1979)
- Singh L, "Genetic differences in soybean trypsin inhibitors separated by disc electrophoresis", Crop Sci, 9, pp.489~491 (1969)
- Mi-Kyung Sung, "Inheritance of Cgy1 gene and Ti gene in Mature Soybean Seed", 한국육종학회지, 42(1), pp.35~39 (2010), 한국육종학회
- Thanh VH, "Heterogeneity of beta-conglycinin", Biochim Biophys Acta, 469, pp.326~328 (1976)