당근의 약배양으로부터 직접 및 간접 배형성을 통한 식물체 생산 체계 확립
Establishment of Plant Production System through Direct and Indirect Embryogenesis from Anther Culture of Carrot
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We tried to develop the protocol for embryogenesis and plant regeneration from anther culture of carrot (Daucus carotaL.) genotype ‘S&P2342’. Anthers were cultured on MS medium with B5 vitamins containing different combinations of 2,4-Dand NAA for 18 weeks in the dark. The best induction of callus and embryo was obtained in the medium containing 0.1 mg/L2,4-D and 0.1 mg/L NAA, on which 22.0% callus and 2.0% embryo were induced. When primary embryos induced directly fromanther culture were transferred to the regeneration medium, secondary embryos were initiated from primary embryos after 4weeks of culture and 62.5% converted into plantlets after 8 weeks of culture. The plantlets with true leaves were obtained after12 weeks of culture. When the calli derived from anther culture were transferred to the regeneration medium, 38.8% of the calliproduced primary embryos and plantlets after 8 weeks of culture. The plantlets with 2 or more leaves cultured on the regenerationunder the different light intensity for the growth of in vitro plantlets. The plantlets cultured at 100 μmol･m-2･s-1 showed thehighest growth rate. For the acclimatization, the in vitro plantlets with 4 or more leaves cultivated under the different lightintensity and temperature, respectively. The survival rate and growth of plantlets was best at 15℃ and 100 μmol･m-2･s-1,respectively. The plants were successfully acclimatized and had a normal phenotype. The anther culture system could be usedto prepare the doubled haploid lines as an appropriate breeding material for F1 hybrid breeding program.
Abstract
We tried to develop the protocol for embryogenesis and plant regeneration from anther culture of carrot (Daucus carotaL.) genotype ‘S&P2342’. Anthers were cultured on MS medium with B5 vitamins containing different combinations of 2,4-Dand NAA for 18 weeks in the dark. The best induction of callus and embryo was obtained in the medium containing 0.1 mg/L2,4-D and 0.1 mg/L NAA, on which 22.0% callus and 2.0% embryo were induced. When primary embryos induced directly fromanther culture were transferred to the regeneration medium, secondary embryos were initiated from primary embryos after 4weeks of culture and 62.5% converted into plantlets after 8 weeks of culture. The plantlets with true leaves were obtained after12 weeks of culture. When the calli derived from anther culture were transferred to the regeneration medium, 38.8% of the calliproduced primary embryos and plantlets after 8 weeks of culture. The plantlets with 2 or more leaves cultured on the regenerationunder the different light intensity for the growth of in vitro plantlets. The plantlets cultured at 100 μmol･m-2･s-1 showed thehighest growth rate. For the acclimatization, the in vitro plantlets with 4 or more leaves cultivated under the different lightintensity and temperature, respectively. The survival rate and growth of plantlets was best at 15℃ and 100 μmol･m-2･s-1,respectively. The plants were successfully acclimatized and had a normal phenotype. The anther culture system could be usedto prepare the doubled haploid lines as an appropriate breeding material for F1 hybrid breeding program.
참고문헌 (20)
- Alessandro MS, "Inheritance of vernalization requirement in carrot", J. Am. Soc. Hortic. Sci, 132, pp.525~529 (2007)
- Andersen SB, "Biotechnology in agriculture and forestry, Vol 12. Haploids in crop improvement I", pp.393~402 (1990), Springer
- 조문수, "당근 약배양에 의한 식물체 재분화 및 순화", Journal of Plant Biotechnology, 30(1), pp.47~52 (2003), 한국식물생명공학회
- Flehinghaus T, "Methodical improvements in rye anther culture", Plant Cell Rep, 10, pp.397~400 (1991)
- Gamborg OL, "Nutrient requirements of suspension cultures of soybean root cell", Exp. Cell Res, 50, pp.151~158 (1968)
- Gorecka K, "The influence of several factors on the efficiency of androgenesis in carrot", J. Appl. Genet, 46, pp.265~269 (2005)
- Gorecka K, "Plant regeneration from carrot (Daucus carota L.)anther culture derived embryos", Acta Physiol. Plant, 31, pp.1139~1145 (2009)
- Hu KL, "Haploid plant production by anther culture in carrot (Daucus carota L.)", J. Japan. Soc. Hortic. Sci, 62, pp.561~565 (1993)
- Kielkowska A, "In vitro culture of unfertilized ovules in carrot (Daucus carota L.)", Plant Cell Tissue Organ Cult, 102, pp.309~319 (2010)
- Kim YS., "Development of carrot F1 hybrid varieties having high-yield and late-bolting qualities", 박사, pp.2009
- Kiszczak W, "Determination of ploidy and homozygosity of carrot plants obtained from anther cultures", Acta Physiol. Plant, 33, pp.401~407 (2011)
- 강원희, "Morphological Characterization of Anther Derived Plants in Minipaprika (Capsicum annuum L.)", 한국육종학회지, 44(4), pp.450~461 (2012), 한국육종학회
- Maraschin SF, "Androgenic switch: an example of plant embryogenesis from the male gametophyte perspective", J. Exp. Bot, 56, pp.1711~1726 (2005)
- Matsubara S, "Callus formation and regeneration of adventitious embryos from carrot, fennel and mitsuba microspores by anther and isolated microspore cultures", Acta Hortic, 392, pp.129~137 (1995)
- Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue cultures", Physiol. Plant, 15, pp.473~497 (1962)
- Simon PW, "Plant breeding reviews, Vol. 19", pp.157~189 (2000), John Wiley & Sons, Inc.
- Stein M, "Some remarks on carrot breeding (Daucus carota sativus Hoffm.)", Plant Breed, 114, pp.1~11 (1995)
- Wang M, "Insights into a key developmental switch and its importance for efficient plant breeding", Plant Physiol, 124, pp.523~530 (2000)
- Wehner TC, "The genetics and exploitation of heterosis in crops", pp.387~397 (1999), Am. Soc. Agron
- Yoo KG, "Breeding male sterile lines through anther culture in carrot (Daucus carota L.)", 박사, pp.2010