임산부의 혈중 엽산과 호모시스틴 수준이 태반세포의 DNA 메틸화에 미치는 영향
Folate and Homocysteine Levels during Pregnancy affect DNA Methylation in Human Placenta
0
조회
0
다운로드
1
인용
0
참고문헌
초록
Abstract
Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Womanကs University Hospital for prenatal care during their 24~28 weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations. The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.
참고문헌 (31)
- "DNA methylationin health and disease", 1, pp.11~19 (2000)
- "Epigenetics:regulationthrough repression", 286, pp.481~486 (1999)
- "Enzymatic methylesterification of erythrocyte membrane proteinsis impaired in chronic renal failure:evidence forhigh levels of the natural inhibitor Sadenosylhomocysteine", 91, pp.2497~2503 (1993)
- "Inhibition of growth andp21ras methylation in vascular endothelial cellsby homocysteine but not cysteine", 272, pp.25380~25385
- "Mice deficient in methylenetetrahydrofolatereductase exhibit hyperhomocysteinemia anddecreased methylation capacity", 10, pp.433~443
- "Hyperhomocysteinaemiaand vascular disease:a role for DNAhypomethylation", 361, pp.17~ (2003)
- "Biological and clinical implicationsof the MTHFR C677T polymorphism", 22, pp.195~201 (2002)
- "and susceptibility to preeclampsia", 6, pp.74~79
- "10-Methylenetetrahydrofolatereductase polymorphism with folate andvitamin B12 Intakes and serum homocysteineconcentrations in pregnant women", 35(10), pp.1045~1052
- "Moderate folate depletionincreases plasma homocysteine and decreaseslymphocyte DNA methylation in postmenopausal women", 128, pp.1204~1212 (1998)
- "Impact of Dnmt1 deficiency,withand without low folate diets,on tumor numbersand DNA methylation in Min mice", 24, pp.39~45 (2003)
- "Cancer as anepigenetic disease:DNA methylation andchromatin alterations in human tumours", 196, pp.1~7 (2002)
- "DNA methylation and atherosclerosis", 132
- "DNAmethylation,smooth muscle cells,andatherogenesis", 23, pp.1750~1753
- "A methylated oligonucleotide inhibitsIGF2 expression and enhances survival in amodel of hepatocellular carcinoma", 111, pp.265~273 (2003)
- "Short-chainfatty acid inhibitors of histone deacetylases:promising anticancer therapeutics", 3, pp.219~236 (2003)
- "Local hypomethylation inatherosclerosis found in rabbit ec-sod gene", 19, pp.2171~2178
- "DNAhypomethylation and methyltransferaseexpression in atherosclerotic lesions", 7, pp.5~11
- "Increased homocysteineand S-adenosylhomocysteine concentrationsand DNA hypomethylation in vascular disease", 49, pp.1292~1296 (2003)
- "Hyperhomocysteinemia,oxidative stress,and cerebral vasculardysfunction", 35, pp.345~347 (2004)
- "Folic acid,polymorphismof methyl-group metabolism genes,and DNAmethylation in relation to GI carcinogenesis", 38, pp.821~829 (2003)
- "folate and vitamin B12deficiencies and hyperhomocysteinaemiaduring pregnancy associated with shortgestational age and reduced birth weight", 23, pp.1~6
- "and histone acetylation in IUGR rats", 18(1), pp.43~50
- "Methylation and the genome.thepower of a small amendment", 132 (2002)
- "Determination of free andtotal homocysteine in human plasma by highperformance liquid chromatography withfluorescence detection", 422, pp.43~52 (1987)
- "Immunohistochemical evaluation of globalDNA methylation", 75(6), pp.251~258 (2000)
- "Folate deficiency inducedhomocysteinaemia in rats:disruption of Sadenosylmethionine=s co-ordinate regulationof homocysteine metabolism", 298, pp.415~419 (1994)
- "Molecular and cytological evidence of Sadenosyl-L-homocysteine as an innocuousundermethylating agent in vivo", 71, pp.187~192 (1995)
- "Effects of theinteraction between the C677T 5,10-methylenetetrahydrofolate reductase polymorphismand serum B vitamins on homocysteinelevels in pregnant women", 58, pp.10~16
- "Methylenetetrahydrofolatereductase,diet and risk of colon cancer", 8, pp.513~518 (1999)
- "Maternalexposure to environmental tobaccosmoke low birthweight or preterm baby", 36(2), pp.117~124
