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임산부의 혈중 엽산과 호모시스틴 수준이 태반세포의 DNA 메틸화에 미치는 영향

Folate and Homocysteine Levels during Pregnancy affect DNA Methylation in Human Placenta

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Abstract

Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Womanကs University Hospital for prenatal care during their 24~28 weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations. The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.

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관련 논문AI추천

한국인 자간전증 임신부에 있어서 C677T Methylene-tetrahydrofolate reductase 돌연변이에 의한혈청내 호모시스테인과 엽산의 변화에 관한 연구김기남, 김영주, 김우경, 박미혜, 박혜숙, 방명걸, 이보은, 장남수, 전선희, 하은희대한산부인과학회 · Obstetrics & Gynecology Science2004 · 제47권 제7호중증 자간전증이 동반된 임산부에서혈중 homocysteine치 측정의 의의김행수, 양정인, 유희석, 이희종, 임종찬대한산부인과학회 · Obstetrics & Gynecology Science2004 · 제47권 제3호Homocysteine과 血中 脂質濃度와의 相關性 硏究고영철, 김성철, 박재현, 조남근, 황우준대한한방내과학회 · 대한한방내과학회지2006 · 제27권 제3호Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FRα, IGF-2 and IGF-1R in the postnatal rat liver김혜원, 임은미, 장남수, Karen Kay Mejos한국영양학회 · Nutrition Research and Practice2013 · 제7권 제4호Combined effect of folate and adiposity on homocysteine in children at three years of age김영주, 박보현, 박은애, 박혜숙, 이혜아, 조수진, 하은희한국영양학회 · Nutrition Research and Practice2016 · 제10권 제1호Effect of paternal folate deficiency on placental folate content and folate receptor α expression in rats김혜원, 장남수, 최연정, Ki Nam Kim, Tsunenobu Tamura한국영양학회 · Nutrition Research and Practice2011 · 제5권 제2호

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