Effects of parental folate deficiency on the folate content, global DNA methylation, and expressions of FRα, IGF-2 and IGF-1R in the postnatal rat liver
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FRα), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented,PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: PSxMS, PSxMD, PDxMS, and PDxMD. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the PDxMD and PSxMD and PDxMS as compared with the PSxMS group. The hepatic global DNA methylation was decreased in the PDxMS and PSxMD groups as much as in the PDxMD group,and all the three groups were significantly lower as compared to the PSxMS group. There were no significant differences in the hepatic FRα, IGF-2and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FRα, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.
Abstract
We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FRα), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented,PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: PSxMS, PSxMD, PDxMS, and PDxMD. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the PDxMD and PSxMD and PDxMS as compared with the PSxMS group. The hepatic global DNA methylation was decreased in the PDxMS and PSxMD groups as much as in the PDxMD group,and all the three groups were significantly lower as compared to the PSxMS group. There were no significant differences in the hepatic FRα, IGF-2and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FRα, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.
참고문헌 (34)
- Tamura T, "Folate and human reproduction", Am J Clin Nutr, 83, pp.993~1016 (2006)
- Pufulete M, "Effect of folic acid supplementation on genomic DNA methylation in patients with colorectal adenoma", Gut, 54, pp.648~653 (2005)
- Cetin I, "Role of micronutrients in the periconceptional period", Hum Reprod Update, 16, pp.80~95 (2010)
- Farin CE, "Expression of messenger RNAs for insulin-like growth factors and their receptors in bovine fetuses at early gestation from embryos produced in vivo or in vitro", Theriogenology, 74, pp.1288~1295 (2010)
- Liu JP, "Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r)", Cell, 75, pp.59~72 (1993)
- Pedone PV, "Parental imprinting of rat insulin-like growth factor II gene promoters is coordinately regulated", J Biol Chem, 269, pp.23970~23975 (1994)
- Overall M, "Genomic imprinting in the rat: linkage of Igf2 and H19 genes and opposite parental allele-specific expression during embryogenesis", Genomics, 45, pp.416~420 (1997)
- Randhawa R, "The role of the insulin-like growth factor system in prenatal growth", Mol Genet Metab, 86, pp.84~90 (2005)
- Wolf E, "What is the function of IGF-II in postnatal life? Answers from transgenic mouse models", Growth Horm IGF Res, 8, pp.185~193 (1998)
- Qiu Q, "Activation of endoproteolytic processing of insulin-like growth factor-II in fetal, early postnatal, and pregnant rats and persistence of circulating levels in postnatal life", Endocrinology, 148, pp.4803~4811 (2007)
- Marks AG, "Plasma distribution and signaling activities of IGF-II precursors", Endocrinology, 152, pp.922~930 (2011)
- Morison IM, "Imprinting of insulin-like growth factor 2 is modulated during hematopoiesis", Blood, 96, pp.3023~3028 (2000)
- Wani NA, "Decreased activity of folate transporters in lipid rafts resulted in reduced hepatic folate uptake in chronic alcoholism in rats", Genes Nutr, 8, pp.209~219 (2013)
- Mato JM, "Methionine metabolism and liver disease", Annu Rev Nutr, 28, pp.273~293 (2008)
- Zhao R, "Mechanisms of membrane transport of folates into cells and across epithelia", Annu Rev Nutr, 31, pp.177~201 (2011)
- Lillycrop KA, "Dietary protein restriction of pregnant rats induces and folic acid supplementation prevents epigenetic modification of hepatic gene expression in the offspring", J Nutr, 135, pp.1382~1386 (2005)
- Howell CY, "Genomic imprinting disrupted by a maternal effect mutation in the Dnmt1 gene", Cell, 104, pp.829~838 (2001)
- Haggarty P, "Folate in pregnancy and imprinted gene and repeat element methylation in the offspring", Am J Clin Nutr, 97, pp.94~99 (2013)
- Bourque DK, "Decreased placental methylation at the H19", IGF2 imprinting control region is associated with normotensive intrauterine growth restriction but not preeclampsia, 2010, pp.31, 197~202
- Braunschweig MH, "Relationship of porcine IGF2 imprinting status to DNA methylation at the H19 DMD and the IGF2 DMRs 1 and 2", BMC Genet, 12, pp.47~ (2011)
- Gong L, "Gestational low protein diet in the rat mediates Igf2 gene expression in male offspring via altered hepatic DNA methylation", Epigenetics, 5, pp.619~626 (2010)
- Steegers-Theunissen RP, "Periconceptional maternal folic acid use of 400 microg per day is related to increased methylation of the IGF2 gene in the very young child", PLoS One, 4, pp.e7845~ (2009)
- Hoyo C, "Methylation variation at IGF2 differentially methylated regions and maternal folic acid use before and during pregnancy", Epigenetics, 6, pp.928~936 (2011)
- Hansen M, "The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization", N Engl J Med, 346, pp.725~730 (2002)
- Carrell DT, "The human sperm epigenome and its potential role in embryonic development", Mol Hum Reprod, 16, pp.37~47 (2010)
- Virro MR, "Sperm chromatin structure assay (SCSA) parameters are related to fertilization, blastocyst development, and ongoing pregnancy in in vitro fertilization and intracytoplasmic sperm injection cycles", Fertil Steril, 81, pp.1289~1295 (2004)
- Jenkins TG, "The sperm epigenome and potential implications for the developing embryo", Reproduction, 143, pp.727~734 (2012)
- 김혜원, "Effect of paternal folate deficiency on placental folate content and folate receptor α expression in rats", Nutrition Research and Practice, 5(2), pp.112~116 (2011), 한국영양학회
- Kim HW, "Effects of paternal folate deficiency on the expression of insulin-like growth factor-2 and global DNA methylation in the fetal brain", MOLECULAR NUTRITION FOOD RESEARCH, 57(4), pp.671~676 (2013), WILEY-BLACKWELL
- Baker H, "Role of placenta in maternal-fetal vitamin transfer in humans", Am J Obstet Gynecol, 141, pp.792~796 (1981)
- Solanky N, "Expression of folate transporters in human placenta and implications for homocysteine metabolism", Placenta, 31, pp.134~143 (2010)
- Ball GF, "Vitamins in Foods: Analysis, Bioavailability, and Stability", pp.2006, CRC
- Molloy AM, "Maternal and fetal plasma homocysteine concentrations at birth: the influence of folate, vitamin B12, and the 5,10- methylenetetrahydrofolate reductase 677C-->T variant", Am J Obstet Gynecol, 186, pp.499~503 (2002)
- Waterland RA, "Epigenetic epidemiology of the developmental origins hypothesis", Annu Rev Nutr, 27, pp.363~388 (2007)
