Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat
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Objective: The aim of this study was to evaluate the effects of vitamin E (α-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. Methods: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of α-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg α-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: New bone area, bone perimeter, feret’s diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p ＜ 0.001). Bone architecture in α-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. Conclusions: The application of α-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.
Abstract
Objective: The aim of this study was to evaluate the effects of vitamin E (α-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. Methods: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of α-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg α-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: New bone area, bone perimeter, feret’s diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p ＜ 0.001). Bone architecture in α-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. Conclusions: The application of α-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.
참고문헌 (31)
- Saito S, "Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat", Am J Orthod Dentofacial Orthop, 111, pp.525~532 (1997)
- Sawada M, "Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-β1 in the rat", Eur J Orthod, 18, pp.169~179 (1996)
- Uysal T, "Stimulation of bone formation by dietary boron in an orthopedically expanded suture in rabbits", Angle Orthod, 79, pp.984~990 (2009)
- Uysal T, "Effect of ED-71, a new active vitamin D analog, on bone formation in an orthopedically expanded suture in rats. a histomorphometric study", Eur J Dent, 3, pp.165~172 (2009)
- Ten Cate AR, "Sutural development: structure and its response to rapid expansion", Am J Orthod, 71, pp.622~636 (1977)
- Mundy GR, "Cytokines and growth factors in the regulation of bone remodeling", J Bone Min Res, 8, pp.S505~S510 (1993)
- Key LL, "Oxygen derived free radicals in osteoclasts: the specificity and location of the nitroblue tetrazolium reaction", Bone, 11, pp.115~119 (1990)
- Göktürk E, "Oxygen-free radicals impair fracture healing in rats", Acta Orthop Scand, 66, pp.473~475 (1995)
- Turk C, "Promotion of fracture healing by vitamin E in rats", J Int Med Res, 32, pp.507~512 (2004)
- Durak K, "Histological assessment of the effect of alpha-tocopherol on fracture healing in rabbits", J Int Med Res, 31, pp.26~30 (2003)
- Durak K, "Antioxidant effect of alpha-tocopherol on fracture haematoma in rabbits", J Int Med Res, 24, pp.419~424 (1996)
- Yilmaz C, "The contribution of vitamin C to healing of experimental fractures", Arch Orthop Trauma Surg, 121, pp.426~428 (2001)
- Burton GW, "Vitamin E as an in vitro and in vivo antioxidant", Ann N Y Acad Sci, 570, pp.7~22 (1989)
- Burton GW, "Vitamin E: antioxidant activity, biokinetics, and bioavailability", Annu Rev Nutr, 10, pp.357~382 (1990)
- van Tits LJ, "Alpha-tocopherol supplementation decreases production of superoxide and cytokines by leukocytes ex vivo in both normolipidemic and hypertriglyceridemic individuals", Am J Clin Nutr, 71, pp.458~464 (2000)
- Manolagas SC, "Bone marrow, cytokines, and bone remodeling. Emerging insights into the pathophysiology of osteoporosis", N Engl J Med, 332, pp.305~311 (1995)
- Turan B, "Selenium combined with vitamin E and vitamin C restores structural alterations of bones in heparin-induced osteoporosis", Clin Rheumatol, 22, pp.432~436 (2003)
- Turan B, "Effect of dietary selenium and vitamin E on the biomechanical properties of rabbit bones", Clin Rheumatol, 16, pp.441~449 (1997)
- "U.S. National Institutes of Health, Bethesda, Maryland, USA"[URL]
- Graber TM, "Orthodontics: current principles and techniques. 4th ed", pp.257~262 (2005), Elsevier Mosby
- Haas AJ, "The treatment of maxillary deficiency by opening the midpalatal suture", Angle Orthod, 35, pp.200~217 (1965)
- Arjmandi BH, "Vitamin E improves bone quality in the aged but not in young adult male mice", J Nutr Biochem, 13, pp.543~549 (2002)
- Arjmandi BH, "Effects of isoflavones, vitamin E, and their combination on bone in an aged rat model of osteopenia", J Bone Min Res, 16, pp.S533~ (2001)
- Swennen G, "Cranio-facial distraction osteogenesis: a review of the literature. Part II: experimental studies", Int J Oral Maxillofac Surg, 31, pp.123~135 (2002)
- Storey E, "Tissue response to the movement of bones", Am J Orthod, 64, pp.229~247 (1973)
- Hagiwara T, "Effect of electrical stimulation on mandibular distraction osteogenesis", J Craniomaxillofac Surg, 28, pp.12~19 (2000)
- Eriksen EF, "Bone histology and histomorphometry. In: Bone histomorphometry", pp.33~38 (1994), Raven Press
- Burstone CJ, "Sutural expansion by controlled mechanical stress in the rat", J Dent Res, 38, pp.534~540 (1959)
- Cope JB, "Mineralization dynamics of regenerate bone during mandibular osteodistraction", Int J Oral Maxillofac Surg, 30, pp.234~242 (2001)
- Sencimen M, "Histomorphometrical analysis of new bone obtained by distraction osteogenesis and osteogenesis by periosteal distraction in rabbits", Int J Oral Maxillofac Surg, 36, pp.235~242 (2007)
- Garrett IR, "Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo", J Clin Invest, 85, pp.632~639 (1990)