Stimulation of bone formation by direct electrical current in an orthopedically expanded suture in the rat
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. Methods: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure 10μA continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (μm2), bone perimeter (μm), feret’s diameter (μm) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret’s diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. Conclusions: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone. (Korean J Orthod 2010;40(2):106-114)
Abstract
Objective: The aim of this experimental study was to evaluate the effects of direct electrical current stimulation (DECS) on bone regeneration in response to an expansion of the inter-premaxillary suture in the rat. Methods: Sixteen 50 - 60 days old Wistar male rats were separated into two equal groups (control and experimental). Both groups were subjected to expansion, and 30-gram of force was applied to the maxillary incisors with helical-spring. In the experimental group, two metallic-screws were placed at lateral parts of the maxillary segments. Electrodes were connected to the screws. The device was activated with current adjustment to measure 10μA continuously and the current was monitored daily during the expansion and early-retention phase. Bone regeneration in the sutural area was histomorphometrically evaluated including new-bone area (μm2), bone perimeter (μm), feret’s diameter (μm) and newly formed bone (%) parameters. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: Statistical analysis showed significant differences between groups for all investigated histomorphometric parameters. New bone area (p = 0.002), bone perimeter (p = 0.004), feret’s diameter (p = 0.002) and newly formed bone percentage (p = 0.002) measurements were significantly higher in the experimental group than the control group. Bone histomorphometric measurements revealed that bone architecture in the DECS group was improved. Conclusions: The application of DECS to an orthopedically expanded inter-premaxillary suture area during the early retention phase stimulated the formation of new bone. (Korean J Orthod 2010;40(2):106-114)
참고문헌 (24)
- InHoChoi, "Angiogenesis and Mineralization During Distraction Osteogenesis", Journal of Korean Medical Science, 17(4), pp.435~447 (2002), 대한의학회
- Krebs AA, "Midpalatal suture expansion studied by the implant method over a seven-year period", Trans Eur Orthod Soc, 40, pp.131~42 (1964)
- Vardimon AD, "Stability of magnetic versus mechanical palatal expansion", Eur J Orthod, 11, pp.107~15 (1989)
- Timms DJ, "Long term follow-up of cases treated by rapid maxillary expansion", Trans Eur Orthod Soc, 52, pp.211~5 (1976)
- Haas AJ, "The treatment of maxillary deficiency by opening the midpalatal suture", Angle Orthod, 35, pp.200~17 (1965)
- Saito S, "Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat", Am J Orthod Dentofacial Orthop, 111, pp.525~32 (1997)
- Sawada M, "Stimulation of bone formation in the expanding mid-palatal suture by transforming growth factor-beta 1 in the rat", Eur J Orthod, 18, pp.169~79 (1996)
- Uysal T, "Stimulation of bone formation by dietary boron in an orthopedically expanded suture in rabbits", Angle Orthod, 79, pp.984~90 (2009)
- Uysal T, "Effect of ED-71, a new active vitamin D analog, on bone formation in an orthopedically expanded suture in rats. A histomorphometric study. Eur J Dent 2009;3:165-72", Eur J Dent, 3, pp.165~72 (2009)
- Tancan Uysal, "Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat", 대한치과교정학회지, 39(5), pp.337~347 (2009), 대한치과교정학회
- Albert SF, "Electrical stimulation of bone repair", Clin Podiatr Med Surg, 8, pp.923~35 (1991)
- Blank M, "The influence of surface charge on oligomeric reactions as a basis for channel dynamics. In Mechanistic Approaches to interactions of electric and electromagnetic fields with living systems", pp.151~60 (1987), Plenum Press
- Block MS, "Use of distraction osteogenesis for maxillary advancement: preliminary results", J Oral Maxillofac Surg, 52, pp.282~6 (1994)
- Brighton CT, "A multicenter study of the treatment of non-union with constant direct current", J Bone Joint Surg Am, 63, pp.2~13 (1981)
- Cane V, "PEMFs modulate the enzymatic activity during the bone repair process", Bone, 19, pp.133S~ (1996)
- 김동환, "초소형 전기장치에 의한 미세 전류가 치아이동에 미치는 효과", 대한치과교정학회지, 38(5), pp.337~346 (2008), 대한치과교정학회
- El-Hakim IE, "Preliminary investigation into the effects of electrical stimulation on mandibular distraction osteogenesis in goats", Int J Oral Maxillofac Surg, 33, pp.42~7 (2004)
- Hagiwara T, "Effect of electrical stimulation on mandibular distraction osteogenesis", J Craniomaxillofac Surg, 28, pp.12~9 (2000)
- "U.S. National Institutes of Health, Bethesda, Maryland, USA"[URL]
- Swennen G, "Cranio-facial distraction osteogenesis: a review of the literature. Part II: experimental studies", Int J Oral Maxillofac Surg, 31, pp.123~35 (2002)
- Storey E, "Tissue response to the movement of bones", Am J Orthod, 64, pp.229~47 (1973)
- Burstone CJ, "Sutural expansion by controlled mechanical stress in the rat", J Dent Res, 38, pp.534~40 (1959)
- Cope JB, "Mineralization dynamics of regenerate bone during mandibular osteodistraction", Int J Oral Maxillofac Surg, 30, pp.234~42 (2001)
- Matsunaga S, "Histological, histochemical investigations of constant direct current stimulated intramedullary callus", Nippon Seikeigeka Gakkai Zasshi, 60, pp.1293~303 (1986)