Effect of nicotine on orthodontic tooth movement and bone remodeling in rats
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objective: To quantitatively analyze the effect of nicotine on orthodontic tooth movement (OTM) and bone remodeling in rats using micro-computed tomography and tartrate-resistant acid phosphatase immunostaining. Methods: Thirty-nine adult male Sprague–Dawley rats were randomized into three groups: group A, 0.5 mL normal saline (n = 9, 3 per 3, 7, and 14 days); group B, 0.83 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days); and group C, 1.67 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days). Each animal received daily intraperitoneal injections of nicotine/saline from the day of insertion of identical 30-g orthodontic force delivery systems. A 5-mm nickel-titanium closed-coil spring was applied between the left maxillary first molar (M1) and the two splinted incisors. The rate of OTM and volumetric bone changes were measured using micro-computed tomography. Osteoclasts were counted on the mesial alveolar bone surface of the distobuccal root of M1. Six dependent outcome variables, including the intermolar distance, bone volume fraction, bone mineral density, trabecular thickness, trabecular volume, and osteoclast number, were summarized using simple descriptive statistics. Nonparametric Kruskal–Wallis tests were used to evaluate differences among groups at 3, 7, and 14 days of OTM. Results: All six dependent outcome variables showed no statistically significant among group-differences at 3, 7, and 14 days. Conclusions: The findings of this study suggest that nicotine does not affect OTM and bone remodeling, although fluctuations during the different stages of OTM in the nicotine groups should be elucidated in further prospective studies.
Abstract
Objective: To quantitatively analyze the effect of nicotine on orthodontic tooth movement (OTM) and bone remodeling in rats using micro-computed tomography and tartrate-resistant acid phosphatase immunostaining. Methods: Thirty-nine adult male Sprague–Dawley rats were randomized into three groups: group A, 0.5 mL normal saline (n = 9, 3 per 3, 7, and 14 days); group B, 0.83 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days); and group C, 1.67 mg/kg nicotine (n = 15, 5 per 3, 7, and 14 days). Each animal received daily intraperitoneal injections of nicotine/saline from the day of insertion of identical 30-g orthodontic force delivery systems. A 5-mm nickel-titanium closed-coil spring was applied between the left maxillary first molar (M1) and the two splinted incisors. The rate of OTM and volumetric bone changes were measured using micro-computed tomography. Osteoclasts were counted on the mesial alveolar bone surface of the distobuccal root of M1. Six dependent outcome variables, including the intermolar distance, bone volume fraction, bone mineral density, trabecular thickness, trabecular volume, and osteoclast number, were summarized using simple descriptive statistics. Nonparametric Kruskal–Wallis tests were used to evaluate differences among groups at 3, 7, and 14 days of OTM. Results: All six dependent outcome variables showed no statistically significant among group-differences at 3, 7, and 14 days. Conclusions: The findings of this study suggest that nicotine does not affect OTM and bone remodeling, although fluctuations during the different stages of OTM in the nicotine groups should be elucidated in further prospective studies.
참고문헌 (30)
- César-Neto JB, "A comparative study on the effect of nicotine administration and cigarette smoke inhalation on bone healing around titanium implants", J Periodontol, 74, pp.1454~1459 (2003)
- Hapidin H, "Negative effects of nicotine on bone-resorbing cytokines and bone histomorphometric parameters in male rats", J Bone Miner Metab, 25, pp.93~98 (2007)
- Suda N, "Participation of oxidative stress in the process of osteoclast differentiation", Biochim Biophys Acta, 1157, pp.318~323 (1993)
- Mody N, "Oxidative stress modulates osteoblastic differentiation of vascular and bone cells", Free Radic Biol Med, 31, pp.509~519 (2001)
- Bosco AF, "A histologic and histometric assessment of the influence of nicotine on alveolar bone loss in rats", J Periodontol, 78, pp.527~532 (2007)
- Liu YF, "Microcomputerized tomography analysis of alveolar bone loss in ligature-and nicotine-induced experimental periodontitis in rats", J Periodontal Res, 45, pp.714~719 (2010)
- César-Neto JB, "The influence of cigarette smoke inhalation and its cessation on the tooth-supporting alveolar bone : a histometric study in rats", J Periodontal Res, 41, pp.118~123 (2006)
- Krishnan V, "Cellular, molecular, and tissue-level reactions to orthodontic force", Am J Orthod Dentofacial Orthop, 129, pp.469.e1~461 (2006)
- Gameiro GH, "The influence of drugs and systemic factors on orthodontic tooth movement", J Clin Orthod, 41, pp.73~78 (2007)
- Iino S, "Acceleration of orthodontic tooth movement by alveolar corticotomy in the dog", Am J Orthod Dentofacial Orthop, 131, pp.448.e1~8 (2007)
- Yadav S, "Effect of low-frequency mechanical vibration on orthodontic tooth movement", Am J Orthod Dentofacial Orthop, 148, pp.440~449 (2015)
- Sodagar A, "Effect of nicotine on orthodontic tooth movement in rats", Am J Orthod Dentofacial Orthop, 139, pp.e261~5 (2011)
- Bakathir MA, "Effects of nicotine on bone during orthodontic tooth movement in male rats. Histological and immunohistochemical study", Saudi Med J, 37, pp.1127~1135 (2016)
- Shintcovsk RL, "Nicotine effect on bone remodeling during orthodontic tooth movement : histological study in rats", Dental Press J Orthod, 19, pp.96~107 (2014)
- Eckelman WC, "Justifying the number of animals for each experiment", Nucl Med Biol, 34, pp.229~232 (2007)
- Scheibe PO, "Number of samples-hypothesis testing", Nucl Med Biol, 35, pp.3~9 (2008)
- Nociti FH Jr, "Histometric evaluation of the effect of nicotine administration on periodontal breakdown : an in vivo study", J Periodontal Res, 36, pp.361~366 (2001)
- Gonzales C, "Force magnitude and duration effects on amount of tooth movement and root resorption in the rat molar", Angle Orthod, 78, pp.502~509 (2008)
- Reitan K, "Comparative behavior of human and animal tissue during experimental tooth movement", Angle Orthod, 41, pp.1~14 (1971)
- Brudvik P, "The initial phase of orthodontic root resorption incident to local compression of the periodontal ligament", Eur J Orthod, 15, pp.249~263 (1993)
- Ren Y, "The rat as a model for orthodontic tooth movement--a critical review and a proposed solution", Eur J Orthod, 26, pp.483~490 (2004)
- Michelogiannakis D, "Influence of nicotine on orthodontic tooth movement : a systematic review of experimental studies in rats", Arch Oral Biol, 93, pp.66~73 (2018)
- King GJ, "Measuring dental drift and orthodontic tooth movement in response to various initial forces in adult rats", Am J Orthod Dentofacial Orthop, 99, pp.456~465 (1991)
- Bouxsein ML, "Guidelines for assessment of bone microstructure in rodents using micro-computed tomography", J Bone Miner Res, 25, pp.1468~1486 (2010)
- Walker LM, "Nicotinic regulation of c-fos and osteopontin expression in human-derived osteoblast-like cells and human trabecular bone organ culture", Bone, 28, pp.603~608 (2001)
- Henemyre CL, "Nicotine stimulates osteoclast resorption in a porcine marrow cell model", J Periodontol, 74, pp.1440~1446 (2003)
- Kirschneck C, "Orthodontic forces add to nicotineinduced loss of periodontal bone : an in vivo and in vitro study", J Orofac Orthop, 76, pp.195~212 (2015)
- Araujo CM, "Effect of acute administration of nicotine and ethanol on tooth movement in rats", Braz Oral Res, 32, pp.e96~ (2018)
- Lee HJ, "Effects of nicotine on antioxidant defense enzymes and RANKL expression in human periodontal ligament cells", J Periodontol, 80, pp.1281~1288 (2009)
- Norazlina M, "Vitamin E reversed nicotineinduced toxic effects on bone biochemical markers in male rats", Arch Med Sci, 6, pp.505~512 (2010)