Anti-proliferative and Anti-telomerase Activity of Curcuma Rhizome Extract on Oral Squamous Cell Carcinoma and Osteosarcoma Cells
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Anti-proliferation of methanol extract of Curcuma rhizome on oral squamous cell carcinoma (KB) and osteosarcoma (HOS) cells were investigated. In order to elucidate the involvement of telomerase inhibitory activity as a part of anti-proliferative effect of Curcuma rhizome on cancer cells, we measured telomerase activity in Curcuma rhizome extract-treated cancer cells. The concentration inhibited cell proliferation to 50% (IC50) of the methanol extract of Curcuma rhizome against oral squamous cell carcinoma (KB) cells and osteosarcoma (HOS) cells were 21.30 μg/ml and 39.3 μg/ml, respectively. The methanol extract of Curcuma rhizome showed inhibitory telomerase inhibitory effect which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Curcuma rhizome is at least partially due to telomerase inhibitory effect. Five fraction samples were prepared according to its polarity differences and analyzed anti-proliferative effects of each fraction samples on oral squamous cell carcinoma and osteosarcoma cells. Anticancer effect was observed in dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had IC50 value of 23.3 μg/ ml and 10.5 μg/ml against oral squamous cell carcinoma (KB) cells and osteosarcoma (HOS) cells, respectively.
Abstract
Anti-proliferation of methanol extract of Curcuma rhizome on oral squamous cell carcinoma (KB) and osteosarcoma (HOS) cells were investigated. In order to elucidate the involvement of telomerase inhibitory activity as a part of anti-proliferative effect of Curcuma rhizome on cancer cells, we measured telomerase activity in Curcuma rhizome extract-treated cancer cells. The concentration inhibited cell proliferation to 50% (IC50) of the methanol extract of Curcuma rhizome against oral squamous cell carcinoma (KB) cells and osteosarcoma (HOS) cells were 21.30 μg/ml and 39.3 μg/ml, respectively. The methanol extract of Curcuma rhizome showed inhibitory telomerase inhibitory effect which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Curcuma rhizome is at least partially due to telomerase inhibitory effect. Five fraction samples were prepared according to its polarity differences and analyzed anti-proliferative effects of each fraction samples on oral squamous cell carcinoma and osteosarcoma cells. Anticancer effect was observed in dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had IC50 value of 23.3 μg/ ml and 10.5 μg/ml against oral squamous cell carcinoma (KB) cells and osteosarcoma (HOS) cells, respectively.
참고문헌 (24)
- Akiyama, M, "Telomerase inhibitors as anticancer therapy.", 2, pp.567~575 (2002)
- Allsopp, "Telomere length predicts replicative capacity of human fibroblasts.Proc. Natl. Acad. Sci. USA 89", pp.10114~10118 (1992)
- M, "Concurrent radiotherapy and chemotherapy with cisplatin in inoperablesquamous cell carcinoma of the head and neck. An RTOGstudy. Cancer 59", pp.259~265 (1987)
- Blackburn, "Structure and function of telomeres. Nature 350", pp.569~573 (1991)
- Giridharan, P, "Novel substituted methylenedioxy lignan suppresses proliferation of cancer cells by inhibiting telomerase andactivation of c-myc and caspases leading to apoptosis.", 87(p.), pp.98~105 (2002)
- Kang, S. C, "Evalution of oriental medicinal herbs for estrogenic and antiproliferative activities.", 20, pp.1017~1019 (2006)
- Kelland, L. R, "Overcoming the immortality of tumour cells by telomere and telomerase based cancer therapeutics--current status and future prospects.", 41, pp.971~979 (2005)
- Kish, J.A, "Cisplatin and 5-fluorouracil infusion in patients with recurrent and disseminated epidermoid cancer of the head and neck. Cancer", 53, pp.1819~ (1819)
- Kojima, H, "Essential oil constituents from Japanese and Indian Curcuma aromatica rhizomes.", 64, pp.380~381 (1998)
- Lee, J. H, "Chemosensitivity of cisplatin and 5-fluorouracil on oral squamous cell carcinoma cell lines.", 24, pp.165~171 (1998)
- Lee, Y. H, "Studies on anti-oral cancer activities of medicinal plant extracts.", 26, pp.53~58 (2000)
- Liu, J. J, "Anti-proliferation effect of oridonin on HL-60 cells and its mechanism.", 19, pp.134~137 (2004)
- Mallery, S. R, "Formulation and in-vitro and in-vivo evaluation of a mucoadhesive gel containing freeze dried black raspberries implications for oral cancer chemoprivention.", 24, pp.728~737 (2007)
- Manoharan, S, "Evaluation of anticarcinogenic effects of clerodendron inerme on 7, 12-dimethylbenz(a) anthracene-induced hamster buccal pouch carcinogenesis", 47, pp.1038~1043 (2006)
- Miyoshi, Y, "Telomerase activity in oral cancer.", 35, pp.283~289 (1999)
- Mutirangura, A, "Telomerase activity in oral leukoplakia and head and neck squamous cell carcinoma.", 56, pp.3530~3533 (1996)
- Olaussen, K. A, "Telomere and telomerase as targets for anticancer drug development.", 57, pp.191~214 (2006)
- Peterson, "Principles of oral and maxillofacial surgery. J. B. Lippincott", indresa (1992)
- Rhyu, M. S, "Telomeres, telomerase, and immortality", 87, pp.884~894 (1995)
- Silverman, "Epidermiologic and demographic update in oral cancer California and national data-1973 to 1985. J. Am. Dent. Assoc. 120", pp.495~499 (1990)
- Suto, "MTT assay with reference to the clinical effect of chemosensitivity. J. Surg. Oncol. 42", pp.28~32 (1989)
- Tohda. C, "Comparison of anti-inflammatory activities of six Curcuma Rhizomes A possible Curcuminoid-independent pathway mediated by Curcuma phaeocaulis extract.", 3, pp.255~260 (2006)
- Zimmermann. S, "Telomeres and telomerase as targets for cancer therapy.", 64, pp.906~921 (2007)
- Zimmermann. S, "Telomeres and telomerase as targets for cancer therapy.", 64, pp.906~921 (2007)