Apoptotic Effect of Co-treatment with Curcumin and Cisplatin on SCC25 Human Tongue Squamous Cell Carcinoma Cell Line
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Curcumin is a widely used flavoring agent in food, and it hasbeen reported to inhibit cell growth, to induce apoptosis, andto have antitumor activity in many cancers. Cisplatin is one ofthe most potent known anticancer agents and showssignificant clinical activity against a variety of solid tumors. This study was undertaken to investigate the synergisticapoptotic effects of co-treatment with curcumin and cisplatinon human tongue SCC25 cells. To investigate whether theco-treatment efficiently reduced the viability of the SCC25cells compared with the two treatments separately, an MTTassay was conducted. The induction and the augmentation ofapoptosis were confirmed by DNA electrophoresis, Hoechststaining, and an analysis of DNA hypoploidy. Western blot,MMP and immunofluorescence tests were also performed toevaluate the expression levels and the translocation ofapoptosis-related proteins following the co-treatment. In this study, following the co-treatment with curcumin andcisplatin, the SCC25 cells showed several forms of apoptoticmanifestation, such as nuclear condensation, DNAfragmentation, reduction of MMP, increased levels of Bax,decreased levels of Bcl-2, and decreased DNA content. Inaddition, they showed a release of cytochrome c into thecytosol, translocation of AIF and DFF40 (CAD) to the nuclei,and activation of caspase-7, caspase-3, PARP, and DFF45(ICAD). In contrast, separate treatments of 5 μM of curcuminor 4 μg/ml of cisplatin, for 24 hours, did not induce apoptosis. Therefore, our data suggest that combination therapy withcurcumin and cisplatin could be considered as a noveltherapeutic strategy for human oral squamous cell carcinoma.
Abstract
Curcumin is a widely used flavoring agent in food, and it hasbeen reported to inhibit cell growth, to induce apoptosis, andto have antitumor activity in many cancers. Cisplatin is one ofthe most potent known anticancer agents and showssignificant clinical activity against a variety of solid tumors. This study was undertaken to investigate the synergisticapoptotic effects of co-treatment with curcumin and cisplatinon human tongue SCC25 cells. To investigate whether theco-treatment efficiently reduced the viability of the SCC25cells compared with the two treatments separately, an MTTassay was conducted. The induction and the augmentation ofapoptosis were confirmed by DNA electrophoresis, Hoechststaining, and an analysis of DNA hypoploidy. Western blot,MMP and immunofluorescence tests were also performed toevaluate the expression levels and the translocation ofapoptosis-related proteins following the co-treatment. In this study, following the co-treatment with curcumin andcisplatin, the SCC25 cells showed several forms of apoptoticmanifestation, such as nuclear condensation, DNAfragmentation, reduction of MMP, increased levels of Bax,decreased levels of Bcl-2, and decreased DNA content. Inaddition, they showed a release of cytochrome c into thecytosol, translocation of AIF and DFF40 (CAD) to the nuclei,and activation of caspase-7, caspase-3, PARP, and DFF45(ICAD). In contrast, separate treatments of 5 μM of curcuminor 4 μg/ml of cisplatin, for 24 hours, did not induce apoptosis. Therefore, our data suggest that combination therapy withcurcumin and cisplatin could be considered as a noveltherapeutic strategy for human oral squamous cell carcinoma.
참고문헌 (46)
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